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Research Journal of Phytochemistry

Year: 2011 | Volume: 5 | Issue: 2 | Page No.: 98-106
Research Article

Screening of Antioxidant and Antiulcer Potential of Citrullus colocynthis Methanolic Seed Extract

N.S. Gill
Rayat School of Pharmacy, S.B.S. Nagar, Punjab Technical University, Ropar-144533, India
Supreet Kaur
Rayat School of Pharmacy, S.B.S. Nagar, Punjab Technical University, Ropar-144533, India
R. Arora
Rayat School of Pharmacy, S.B.S. Nagar, Punjab Technical University, Ropar-144533, India
Dr. M. Bali
Quest Group of Institutes, Jhanjeri, Punjab Technical University, Mohali, India
Medicinal plants are the backbone of Traditional systems of medicine throughout the world. They are invaluable, incredible and traditional source for the curing various diseases in the form of medicines. The objective of our present study was to evaluate the antioxidant and antiulcerogenic property of Citrullus colocynthis seeds. The motivation behind this study was to highlight the importance of traditionally consumed seeds as a potent source of bioactive compounds. Phytochemical screening of methanolic seed extract showed the presence of various phytoconstituents that are responsible for pharmacological activities. Antioxidant activity of extract was studied spectrophotometrically by 1, 1-diphenyl-2-picryl hydrazyl and Hydrogen Peroxide free radical scavenging method. The methanolic seed extract of Citrullus colocynthis showed maximum percentage inhibition of 79.4 and 72.4% by 1, 1-diphenyl-2-picryl hydrazyl and Hydrogen peroxide method respectively at 300 μg mL-1, as the seed extract showed good antioxidant potential. It was further evaluated for anti-ulcerogenic activity by pyloric ligation induced ulcers model in Wistar albino rats. Citrullus colocynthis (200 mg kg-1) showed maximum inhibition of gastric volume, free acid and total acidity 1.68±0.18, 39.86±3.86 and 61.23±1.87 at dose 200 mg kg-1. The maximum percentage inhibition of the extract by pyloric ligation was 71.57% at a dose of 200 mg kg-1. The results of Present study revealed that the seed extract of Citrullus colocynthis possesses significant antiulcerogenic activity due to its free radical scavenging property.
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References

  1. Bhuiyan, M.A.R. and M.Z. Hoque, 2009. Free radical scavenging activities of Zizypus mauritiana. World J. Agric. Sci., 3: 318-322.
  2. Borikar, V.I., C.R. Jangde, P. Philip and D.S. Rekhe, 2009. Study of antiulcer activity of Bauhinia racemosa Lam in rats. Vet. World, 2: 215-216.
    Direct Link
  3. Bose, M., V.M. Motghare, G.N. Dakhale and A.V. Turankar, 2003. Antiulcer activity of levcromakalim and nicorandil in albino rats: A comparative study. Pol. J. Pharmacol., 55: 91-95.
    PubMed
  4. Chanda, S., R. Dave and M. Kaneria, 2011. In vitro antioxidant property of some Indian medicinal plants. Res. J. Med. Plant, 5: 169-179.
    CrossRefDirect Link
  5. Al-Ghamdi, F.A., H.S. Al-Zahrani and K.H. Al-Amer, 2009. Phytosociological studies of Citrullus colocyanthis L., growing in different altitudinal sites in saudi arabia. Pak. J. Biol. Sci., 10: 779-785.
    CrossRefPubMed
  6. Dallak, M. and I. Bin-Jaliah, 2010. Antioxidant activity of Citrullus colocynthis pulp extract in RBC's of Alloxan induced diabetic rats. Pak. J. Physiol., 6: 1-5.
    Direct Link
  7. Gill, N.S., M. Garg, R. Bansal, S. Sood, A. Muthuraman, M. Bali and P.D. Sharma, 2009. Evaluation of antioxidant and antiulcer potential of Cucumis sativum L. seed extract in rats. Asian J. Clin. Nutr., 1: 131-138.
    CrossRef
  8. Gill, N.S., S. Sood, A. Muthuraman, M. Bali and P.D. Sharma, 2011. Evaluation of antioxidant and anti-ulcerative potential of Citrullus lanatus seed extract in rats. Latin Am. J. Pharm., 30: 429-434.
    Direct Link
  9. Gill, N.S., J. Bajwa, K. Dhiman, P. Sharma and S. Sood et al., 2011. Evaluation of therapeutic potential of traditionally consumed Cucumis melo seeds. Asian J. Plant Sci., 10: 86-91.
    CrossRefDirect Link
  10. Gonzalez, F.G. and L.C. Di-Stasi, 2002. Anti-ulcerogenic and analgesic activities of the leaves of Wilbrandia ebracteata in mice. Phytomedicine, 9: 125-134.
    PubMed
  11. Goswami, S., S. Jain and D. Santani, 1997. Anti-ulcer activity of cromakalim (BRL 34915), a potassium-channel opener, against experimentally induced gastric and duodenal ulcers in rats and guinea-pigs. J. Pharm. Pharmacol., 49: 195-199.
    CrossRef
  12. Harborne, J.B., 1973. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. 1st Edn., Springer, Dordrecht, Netherlands, ISBN: 978-94-009-5921-7, Pages: 278.
    CrossRefDirect Link
  13. Hasan, S.S., S.I. Ahmed, N.I. Bukhari and W.C. Loon, 2009. Use of complementary and alternative medicine among patients with chronic diseases at outpatient clinics. Complement. Ther. Clin. Pract., 15: 152-157.
    CrossRefPubMedDirect Link
  14. Khanahmadi, M. and K. Janfeshan, 2006. Study on antioxidation property of Ferulago angulata plant. Asian J. Plant Sci., 5: 521-526.
    CrossRefDirect Link
  15. Koneru, A., S. Satyanarayana, K. Mukkanti and K.A. Khan, 2011. In vitro antioxidant activity of Itrifal Kishneezi: A unani formulation. Am. J. Drug Discovery Dev., 1: 121-128.
  16. Kumar, S., D. Kumar, M. Jusha, K. Saroha, N. Singh and B. Vashishta, 2008. Antioxidant and free radical scavenging potential of Citrullus colocynthis (L.) Schrad. methanolic fruit extract. Acta Pharm., 58: 215-220.
    CrossRefPubMedDirect Link
  17. Srikanth, J. and P. Muralidharan, 2009. Antiulcer activity of Morinda citrifolia Linn fruit extract. J. Scient. Res., 1: 345-352.
    CrossRefDirect Link
  18. Newman, D.J., G.M. Cragg and K.M. Snader, 2000. The influence of natural products upon drugdiscovery. Nat. Prod. Rep., 17: 215-234.
    CrossRefPubMedDirect Link
  19. Marzouk, B., Z. Marzouk, E. Haloui, N. Fenina, A. Bouraoui and M. Aouni, 2010. Screening of analgesic and anti-inflammatory activities of Citrullus colocynthis from Southern Tunisia. J. Ethnopharmacol., 128: 15-19.
    CrossRef
  20. Oluwole, F.S., B.O. Omolaso and J.A. Ayo, 2007. Methanolic extract of Entandrophragma angolense induces gastric mucus cell counts and gastric mucus secretion. J. Boil. Sci., 7: 1531-1534.
    CrossRefDirect Link
  21. Onasanwo, S.A., N. Singh, A.A. Saba, A.A. Oyagbemi, O.A. Oridupa and G. Palit, 2011. Anti-ulcerogenic and in vitro antioxidant activities of Lagenaria breviflora (LB) whole fruit ethanolic extract in laboratory animals. Pharmacognosy Res., 3: 2-8.
    CrossRefDirect Link
  22. Parmar, N.S. and J.K. Desai, 1993. A review of the current methodology for the evaluation of gastric and duodenal anti-ulcer agents. Indian J. Pharmacol., 25: 120-135.
    Direct Link
  23. Parvez, M., M. Gayasuddin, M. Basheer and K. Janakiraman, 2010. Screening of Piper cubeba (Linn) fruits for anti-ulcer activity. Int. J. Pharmtech. Res., 2: 1128-1132.
    Direct Link
  24. Rahbar, A.R. and I. Nabipour, 2010. The hypolipidemic effect of Citrullus colocynthis on patients with hyperlipidemia. Pak. J. Biol. Sci., 13: 1202-1207.
    CrossRefDirect Link
  25. Rajkapoor, B., R. Anandan and B. Jayakar, 2002. Antiulcer effect of Nigella sativa Linn. against gastric ulcer in rats. Curr. Sci., 82: 177-179.
    Direct Link
  26. Shah, B.N., A.K. Seth and R.V. Desai, 2010. Phytopharmacological profile of Lagenaria siceraria: A review. Asian J. Plant Sci., 9: 152-157.
    CrossRefDirect Link
  27. Shekhawat, N., P.S. Soam, T. Singh and R. Vijayvergia, 2010. Assesment of free radical scavenging activity of crude extract of some medicinal plant. Middle-East J. Sci. Res., 5: 298-301.
  28. Siddiq, A., F. Anwar, M. Manzoor and A. Fatima, 2005. Antioxidant activity of different solvent extracts of Moringa oleifera leaves under accelerated storage of sunflower oil. Asian J. Plant Sci., 4: 630-635.
    CrossRefDirect Link
  29. Sood, S., A. Muthuraman, N.S. Gill, M. Bali and P.D. Sharma, 2010. Effect of Citrus karna peel extract on stress induced peptic ulcer in rat. J. Biol. Sci., 10: 231-236.
    CrossRefDirect Link
  30. Sreejayan, N. and M.N. Rao, 1996. Free radical scavenging activity of curcuminoids. Arzneimittelforschung, 46: 169-171.
    PubMedDirect Link
  31. Shay, H., S.A. Komarow, S.S. Fels, D. Meranze, M. Gruenstein and H. Siplet, 1945. A simple method for the uniform production of gastric ulceration in the rat. Gastroenterology, 5: 43-61.
    Direct Link
  32. Takagi, K., S. Okabe and R. Saziki, 1969. A new method for the production of chronic gastric ulcer in rats and the effect of several drugs on its healing. Jpn. J. Pharmacol., 19: 418-426.
    PubMed
  33. Zamani, M., A.O. Rahimi, R. Mahdavi, M. Nikbakhsh and M.V. Jabbari et al., 2007. Assessment of anti-hyperlipidemic effect of Citrullus colocynthis. Rev. Bras. Farmacogn., 17: 492-496.
    CrossRefDirect Link
  34. Karim, A., M.N. Sohail, S. Munir and S. Sattar, 2011. Pharmacology and phytochemistry of Pakistani herbs and herbal drugs used for treatment of diabetes. Int. J. Pharmacol., 7: 419-439.
    CrossRef
  35. Toyama, J., H. Tanaka, A. Horie, T. Uchiyama and R. Akashi, 2008. Purification and characterization of Anti-H lectin from the seed of Momordica charantia and the inter-specific differences of hemagglutinating activity in Cucurbitaceae. Asian J. Plant Sci., 7: 647-653.
    CrossRefDirect Link

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