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Modulation of Pro-inflammatory Mediators by Eugenol in AlCl3 Induced Dementia in Rats



Yanxin Zhao, Dong Wang, Souravh Bais and Hongxin Wang
 
ABSTRACT

Background and Objective: Alzheimer disease is one of the common types of dementia, which involves progressive neuronal cell's degeneration. The main objective of this study was to evaluate the in vitro anti-choline esterase activity of four phenyl propanoids derivatives (Eugenol, Gallic acid (GA), Sinapic acid and Scopolin) and evaluation of Eugenol in AlCl3 induced dementia in rats. Materials and Methods: The four phenyl propanoids derivatives (Eugenol, Gallic acid (GA), Sinapic acid and Scopolin) were evaluated for in vitro anti-choline esterase activity. For in vivo screening the rats were treated with aluminum chloride at a dose of 175 mg kg–1, for a period of 25 days to produce dementia. Then rats divided in 4 different groups and further evaluated for 10 days of treatment , i.e., Negative control, Standard group and one group received sub maximal dose of rivastigmine along with Eugenol (1.25 mg kg–1 p.o+ 50 mg kg–1 Eugenol, p.o) and other group received Eugenol (50 mg kg–1, p.o) alone. All rats were observed until the 35th day of experimental protocol. The different behavioral and biochemical parameters like GSH, TBARS and Nitrite level were also determined. Neuro-protective activity of eugenol against neuro inflammation produced by AlCl3 was accessed by estimations of two pro inflammatory cytokines (TNF-α, IL-1β) in brain tissues. Results: Rats treated with aluminum chloride (175 mg kg–1, p.o.) produced a significant decline in behavioral and biochemical parameters in rats. The rats treated with Eugenol showed significant reversal of memory deficit Dementia of AD type. Conclusion: The study concluded that Eugenol had a synergistic effect with rivastigmine when used in combination and showed neuro protection against AlCl3 induced dementia of AD type in rats and modulates the neuro inflammation produced by AlCl3.

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  How to cite this article:

Yanxin Zhao, Dong Wang, Souravh Bais and Hongxin Wang, 2019. Modulation of Pro-inflammatory Mediators by Eugenol in AlCl3 Induced Dementia in Rats. International Journal of Pharmacology, 15: 457-464.

DOI: 10.3923/ijp.2019.457.464

URL: https://scialert.net/abstract/?doi=ijp.2019.457.464
 
Received: November 13, 2018; Accepted: January 11, 2019; Published: April 15, 2019


Copyright: © 2019. This is an open access article distributed under the terms of the creative commons attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

INTRODUCTION

Alzheimer’s disease (AD) is a type of neuro-degenerative disease, affecting memory and cognitive functions. The most people affected by this disease are at the age 60 plus. Patients become to enable of functions like judgment, decision making, emotional changes and other behavioral changes. The treatment and regimen of the disease include use of anti-choline esterase drugs like donepezil, rivastigmine and galantamine. As per findings suggested some of the herbals also provide symptomatic relief in the AD Type in rats1-4. The neuro-transmitter, acetylcholine deficiency in AD created an urge among scientists to find the suitable compounds as choline esterase inhibitors to increase the availability of it at the synapse5,6. Now, it becomes a major approach for the treatment and added ground-breaking results in AD treatment in patients of AD7. In a search of suitable bio markers, Reactive Oxygen Species (ROS) also plays an important role to cause cellular degeneration so the use of compounds, which prevent cell damage also showed a better improvement in pathogenesis of AD8,9. The natural anti-oxidants like polyphenols, flavonoids, lignins and stilbene which are part of human diets. Traditionally, their supplements were consumed with large population to cure various diseases and some of them are present in edible food to give a prophylactic response to oxidative stress. These compounds are proven for their interaction with several enzymes10. Several other studies suggested that the interaction of polyphenols with enzymes is due to their complex structure and better bioavailability11-13. So the objective of present study was to screen some of the competent polyphenol molecules (Eugenol, Gallic acid (GA), Sinapic Acid and Scopolin) as anti-choline estarse in Alzheimer’s disease and based on results, the effective one is further evaluated for in vivo activity in rats. The study also evaluated the interaction of Eugenol with standard drug (rivastigmine) available for AD Therapy. Choline esterase is most culprit enzymes in neuro-degenerative disease like dementia of AD type in rats so current study will stabilize the role of Eugenol in AD. The study also provides the protective role of Eugenol in neuro inflammation produced by aluminum chloride in dementia.

MATERIALS AND METHODS

All the work related to this study was conducted in affiliated institutes. The analytical part and animal study was conducted in a month of May-August, 2015 and other work was completed on August, 2018.

Drugs and chemicals: The standard compounds like scopoline (99.7%), gallic acid (99.9%), eugenol (99.7%) and sinapic acid (98.9%) were procured from Sigma Aldrich (Germany) (S and G Lab. supplies). Drug, rivastigmine was provided as a gift sample (Ref. Sun/G/254-5 gm) from Sun pharmaceutical Pvt., Ltd., India. The other chemicals like DTNB, acetylthiocholine iodide, trichloroacetic acid, thiobarbituric acid and sodium carboxy methyl cellulose was procured from Merk and S.D. Fine Chemicals Ltd., (Mumbai) India (Analytical Grade).

In vitro ache assay: This assay was performed as per the method described by Nascimento and Martins11 and modified by Albano et al.12 and Owokotomo et al.13. The samples measured at 412 nm in an X-Rite 640B spectrophotometer (Grand Rapids MI, 49512, USA). Rivastigmine was used as positive control. The percentage inhibition of enzyme activity was calculated by comparison with the negative control. The IC50 values are indicative of 50% inhibition of enzyme hydrolysis, which is determined by spectrophotometry method (Table 1). The samples were tested in triplicates. To calculate the IC50 values, each sample was assayed at 5 concentrations (30, 20, 10, 5 and 2.5 mg mL–1). The IC50 values were obtained from dose effect curves by linear regression. The inhibition factor (strength of inhibition) for test and positive control were calculated using the equation13:

where, A0 was the absorbance of the negative control (enzyme+methanol) and A1 was the absorbance of the sample (enzyme+solution of test). Tests were carried out in triplicate and data were analyzed using descriptive statistics:

where, ICx is the concentration of test substance that inhibited x% of AChE activity.

Animals: The Wistar rats (180-200 g, 7-8 weeks) of either sex were selected and provided 12 h light-dark cycle with free access of food (Standard pellet diet) and water. All animals were trained in noise-free environment and trials were conducted between 9:00 AM-2:00 PM. The experiment was ethically approved and carried out as per official guidelines of experimentation in animals (IAEC/2015/17-18).

Acute toxicity study (LD50): The acute toxicity (LD50) of Eugenol was calculated as per standard guidelines issued by organization for economic cooperation and development (OECD) following the up and down method (OECD guideline No. 423)10-12.

Experimental design
Aluminium chloride-induced dementia:
This model was followed by the methods described by Bais et al.3. Day 1- 4, all animals were gone for training under Morris Water Maze and on 5th day (Prove day), rats were randomly divided in order to separate groups (n = 6) (Table 2). Details of groups given:

Group I: Control group received normal saline (5 mL kg–1, p.o)
Group II: Negative control (II) (AlCl3 induced-untreated rats)
Group III: Standard group (III) [(AlCl3 Induced+ Rivastigmine treated rats-(2.5 mg kg–1, p.o.)]
Group IV: Eugenol alone treated group (AlCl3+Eugenol-50 mg kg–1, p.o. treated rats)
Group V: Eugenol along with sub-maximal dose of Rivastigmine (IV) (AlCl3+Eugenol-50 mg kg–1+ Rivastigmine-1.25 mg kg–1, p.o. treated rats)

All rats were accessed for learning, memory and ambulatory movements. Morris water maze test was used to access the memory deficit caused by neuronal damage. The test was performed via well-known specifications of a water maze consisted of a circular (150 cm diameter and 40 cm height)13. This test were performed on 5, 16, 26 and 36th day of experimental trial and later; various parameters like Time elapsed in escaping to the NW quadrant, i.e., escape latency time (ELT) and total time (TT) time spent in NW quadrant were measured during the retention trials3. The animals were sacrificed to study various biochemical parameters like extent of oxidative stress and acetylcholine esterase (Ache) activity14.

Evaluation
Sampling and preparation of tissue homogenate:
At the end of experiment, rats were dissected and their brain were removed immediately and maintained under temperature of 2-8°C to prevent further enzyme degradation. Then it was washed thoroughly with ice cold isotonic saline. A 10% tissue homogenate was prepared using 0.1 M phosphate buffer (pH 8, stored 2-8°C) and used for various estimations.

Biochemical assessment
Acetyl choline esterase (AchE) level:
The AchE level in brain tissue homogenate samples were processed and determined according to the method described by Morris et al.14 and Kangtao et al.15.

Glutathione (GSH) level: The GSH level in brain tissue homogenate samples were processed and determined according to the method described Beutler et al.16 and Bais and Prashar17 with slight modifications. Results were expressed as nmol of GSH/mg of protein.

Catalase level: The catalase level in brain tissue homogenate samples were determined using method described by Owokotomo et al.13 and Luck 18.

Lipid peroxidation (TBARS) level: The TBARS levels in brain tissue homogenate samples were determined via spectrophotometry method19. The content level was expressed in terms of nmol malondialdehyde.

Table 1: Summary of in vitro AchEI by phenyl propanoids derivative
ND: Not shown in test

Table 2: Effect of eugenol on aluminium chloride induced dementia of Alzheimer’s type in rat using morris water maze (Escape latency time)
Data are Mean±SEM values, n = 6, data were analyzed by one-way ANOVA followed by Tukey-Kramer multiple comparisons test, *p<0.05, **p<0.01, ***p<0.001 and nsp>0.05. aCompared with control, bCompared with inducer, NSNot significant, ELT: Escape latency time

Superoxide dismutase (SOD) level: The SOD level in brain tissue homogenate samples were determined using enzyme diagnostic kits (RANDOX, Ransod enzyme kit, U.K.). This method was based on reaction of generated superoxide radicals with 2-(4-iodophenyl)-3-(4-nitrophenyl)-5- phenyl tetrazolium-chloride to form the red formazan dye20.

Nitrite level: It was measured by the method described by Lyle et al.21.

Estimation of pro-inflammatory cytokines: The TNF-α level and IL-1β were estimated by ELISA kit 13.

Statistics and data analysis: The IC10, IC50 and IC90 were calculated from the dose-response equations obtained in reference to standard compound. All data analysis was done using M.S. Excel software. The experiment data was expressed as means±standard error of the mean (SEM). Data were analyzed via non-parametric analysis of variance by two-way (ANOVA) followed by Turkey multiple comparison tests and other data were evaluated using Graph Pad PRISM Version 7, software. A p-value<0.05 was considered statistically significant.

RESULTS

Effects of phenyl propanoids derivatives on in vitro AchEI capacity: The in vitro AchEI was calculated and presented as IC10, IC50 and IC90. From the experimental findings, gallic acid and sinapic acid showed a potent AchEI activity which was matched with standard rivastigmine. Eugenol showed a potent inhibition at lower concentration with less IC50 values. Other compound like scopoline was not found much potent at lower concentration when compared with rivastigmine (Table 1).

Effects of eugenol on behavioral parameters
Morris water maze test:
All the rats were tested for memory deficit in Morris water maze apparatus with randomized training trials for initial four days (probe day). The negative control group (II) showed memory deficit accessed by raised escape latency time (ELT) on 16th and 25th day and reduced time spent in target quadrant when compared with a control group. The animals treated with Eugenol (50 mg kg–1, p.o.) (Alone) in group IV showed reversal of ELT and TT changes and this effect were found more significant (p<0.01) in Group V, when treated in combination with rivastigmine (Table 2, 3).

Effect of eugenol on AchE level: The AchE level was found higher in aluminum treated (Group II) rats as compared with control rats (Group I). Rivastigmine treated rats (Group III) found less concentration of AchE in brain tissues. The rats treated with Eugenol (Group IV) also showed less concentration but the AchE level was much improved when given in combination (Group V) with rivastigmine and found almost twice to Eugenol alone (Group IV). The response observed in combination Eugenol and rivastigmine (Group IV), (AchE level) gave significant amelioration when compared to negative control (Group II) (Table 4).

Effect of eugenol on biochemical parameters: The effects of Eugenol on the different enzyme's level were determined (Table 4). Aluminum treated (Group II) rats showed increased level of TBARS and Nitrite. The rats treated with rivastigmine (Group III) and Eugenol (Group IV and V) showed significant reduction in raised level of TBARS and nitrite, when compared control rats (Group I) on 36th day of trial.

The level of catalase, SOD and GSH were found reduced in aluminum treated (Group II) rats as compared to control rats, which were further improved by the treatment of Eugenol and rivastigmine.

Effect of eugenol on pro-inflammatory cytokines: The rats affected with aluminium chloride showed increased levels of TNF-α in brain tissues (Fig. 1). This level was further reduced when animals treated with Eugenol when compared with negative control rats.

Table 3: Effect of eugenol on aluminium chloride induced dementia of Alzheimer’s type in rat using morris water maze
Data are Mean±SEM values, n = 6, data were analyzed by one-way ANOVA followed by Tukey-Kramer multiple comparisons test, *p<0.05, **p<0.01, ***p<0.001 and nsp>0.05. aCompared with control, bCompared with inducer, NSNot significant, T.T: Total time

Table 4: Effect of Eugenol on aluminium chloride-induced dementia of Alzheimer’s type in rat using various biochemical parameters
Data are Mean±SEM values, n = 6, data were analyzed by one-way ANOVA followed by Tukey-Kramer multiple comparisons test, *p<0.05, **p<0.01, ***p<0.001 and nsp>0.05, aCompared with control, bCompared with inducer, NSNot significant

Fig. 1: Effect of Eugenol on TNF-α level in brain cortex
 
All values were represented as Mean±SEM, data are Mean±SEM values, n = 6, data were analyzed by one-way ANOVA followed by Tukey-Kramer multiple comparisons test, *p<0.05, **p<0.01, ***p<0.001 and nsp>0.05, aCompared with control, bCompared with inducer, NSNot significant

Fig. 2: Effect of Eugenol on IL-1β level in brain cortex
 
All values were represented as Mean±SEM, data are Mean±SEM values, n = 6, data were analyzed by one-way ANOVA followed by Tukey-Kramer multiple comparisons test, *p<0.05, **p<0.01, ***p<0.001 and nsp>0.05, aCompared with control, bCompared with inducer, NSNot significant

Interleukin-1 (IL-1) level was found high in negative control rats when compared with control rats. The rats treated with Eugenol, either alone or in combination with rivastigmine showed decreased levels of IL-1 level in the rat's brain (Fig. 2).

DISCUSSION

The present study proved the additive effect of Eugenol when given along rivastigmine with fewer side effects. The findings will promote the use of nutraceuticals with synthetic drugs in a regimen of AD disease. The choline esterase inhibitors are the only synthetic drugs approved by Food Drug Administration (FDA). The use of rivastigmine is well-established and proven drug for therapy in AD disease22-27. The pathogenesis of AD disease showed impaired cholinergic transmission, which results in memory deficit. This impairment occurs by two ways, (i) Reduction in Ach release28-32 and (ii) Change in acetyl choline transferase activity, which increases scarcity of Ach33-37. The elevated level of AchE, also increase the progression of AD disease so there always a need of a drug regimen that controls its activity. The study also supports the neuro-protection against pro-inflammatory cytokines (TNF-α, IL-1β) to prevent the neuro-inflammation which is an important factor in pathogenesis of AD35.

Flavonoids and polyphenols are the common anti-oxidants available in a human diet but the concentration may vary according to the sources. The plants with rich polyphenols content must be the priority for researchers to explore. These polyphenols are divided into different classes as phenolic acids, flavonoids, lignins and stilbene. These compounds found abundantly in the plant kingdom with structural similarities, compounds like caffeic acid, gallic acid, p-coumaric acid, vanillic acid, ferulic acid and Eugenol have common carboxylic group which may have interaction with different enzymes38-40. Eugenol belongs to bioactive carboxylic acids and found as a potent inhibitor of AchE. Eugenol acts by reducing oxidative stress so why it found effective against cerebral hypoxia39. The effect activates the cholinergic function to improve memory deficit in aluminum chloride induced dementia41-43.

Current study also showed similar findings with impaired behavioral changes by aluminium chloride administration. The results showed significant interaction of Eugenol with AchE and showed decreased escape latency and increased time spent in target quadrant [NW]. The hypothesis suggested the rivastigmine and Eugenol antagonizes the memory deficit caused by aluminium. The study concludes that the synergistic effect of Eugenol with rivastigmine is due to its chelating effects of the enzyme which is supported by a study published by Birks4 stated, the electrostatic interaction of Eugenol with AchE and confirms its AchEI property. Thus, Eugenol may be considered as a potential candidate in treatment of various memory disorders.

CONCLUSION

The study concluded that Eugenol has significant neuro-protective effects in Dementia of AD type and its synergistic effect is due to its interaction AchE. There are further studies need to be done to explore the molecular mechanism of Eugenol with possible mode of action.

SIGNIFICANCE STATEMENT

This study discovered the mechanism of Eugenol in AD disease and stabilizes the therapeutic interaction with AchE that can be beneficial for other scientist to explore the molecular mechanism of Eugenol interaction. This study will help the researchers to uncover the possible cause of synergistic effect of Eugenol with rivastigmine and help many researchers to design the better therapeutic regimen in AD disease.

ACKNOWLEDGMENT

All the authors wants to thanks all the institutes affiliated to this work and providing necessity research facility and institutional funding for it.

REFERENCES
Alam, M.A., N. Subhan, H. Hossain, M. Hossain, H.M. Reza, M.M. Rahman and M.O. Ullah, 2016. Hydroxycinnamic acid derivatives: A potential class of natural compounds for the management of lipid metabolism and obesity. Nutr. Metab., Vol. 13, No. 1. 10.1186/s12986-016-0080-3

Albano, S., A.S. Lima, M.G., Miguel, L.G. Pedro, J.G. Barroso and A.C. Figueiredo, 2012. Antioxidant, anti-5-lipoxygenase and antiacetylcholinesterase activities of essential oils and decoction waters of some aromatic plants. Rec. Nat. Prod., 6: 35-48.
Direct Link  |  

Ali, A.A., H.I. Ahmed and K. Abu-Elfotuh, 2016. Modeling stages mimic Alzheimer’s disease induced by different doses of aluminum in rats: Focus on progression of the disease in response to time. J. Alzheimer’s Parkinsonism Dementia, Vol. 1, No. 1.

Bais, S. and R. Kumari, 2018. Modulatory effect of sinapic acid in toluene induced dementia of leukoencephalopathy type in wistar rats: A biochemical study. Natl. Prod. J., 8: 153-164.
CrossRef  |  Direct Link  |  

Bais, S. and Y. Prashar, 2015. Identification and characterization of amentoflavone from six species of Juniperus against H2O2 induced oxidative damage in human erythrocytes and leucocytes. Res. J. Phytochem., 9: 41-55.
CrossRef  |  Direct Link  |  

Bais, S., R. Kumari and Y. Prashar, 2017. Therapeutic effect of Sinapic acid in aluminium chloride induced dementia of Alzheimer's type in rats. J. Acute Dis., 6: 154-162.
CrossRef  |  Direct Link  |  

Bais, S., R. Kumari and Y. Prashar, 2018. Ameliorative effect of trans-sinapic acid and its protective role in cerebral hypoxia in aluminium chloride induced dementia of Alzheimer's type. CNS Neurol. Disord.-Drug Targets, 17: 144-154.
CrossRef  |  Direct Link  |  

Bais, S.S. and P.Y. Mali, 2013. Protective effect of Amorphophallus campanulatus tuber extracts against H2O2 induced oxidative damage in human erythrocytes and leucocytes. Int. J. Green Pharm., 7: 111-116.
Direct Link  |  

Beutler, E., O. Duron and B.M. Kelly, 1963. Improved method for the determination of blood glutathione. J. Lab. Clin. Med., 61: 882-888.
PubMed  |  Direct Link  |  

Bharathi, N.M. Shamasundar, T.S. Sathyanarayana Rao, M.D. Naidu, R. Ravid and K.S.J. Rao, 2006. A new insight on Al-maltolate-treated aged rabbit as Alzheimer's animal model. Brain Res. Rev., 52: 275-292.
CrossRef  |  Direct Link  |  

Birks, J., 2006. Cholinesterase inhibitors for Alzheimer’s disease. Cochrane Database Syst. Rev., Vol. 25, No. 1.

Bunzel, M., J. Ralph, H. Kim, F. Lu and S.A. Ralph et al., 2003. Sinapate dehydrodimers and sinapate-ferulate heterodimers in cereal dietary fiber. J. Agric. Food Chem., 51: 1427-1434.
CrossRef  |  Direct Link  |  

Campbell, A., 2002. The potential role of aluminium in Alzheimer's disease. Nephrol. Dial. Transplant, 17: 17-20.
CrossRef  |  Direct Link  |  

Canales, J.J., R. Corbalan, C. Montoliu, M. Llansola and P. Monfort et al., 2001. Aluminium impairs the glutamate-nitric oxide-cGMP pathway in cultured neurons and in rat brain in vivo: Molecular mechanisms and implications for neuropathology. J. Inorganic Biochem., 87: 63-69.
CrossRef  |  Direct Link  |  

Carageorgiou, H., A.C. Sideris, I. Messari, C.I. Liakou and S. Tsakiris, 2008. The effects of rivastigmine plus selegiline on brain acetylcholinesterase, (Na+, K+)-, Mg2+-ATPase activities, antioxidant status and learning performance of aged rats. Neuropsychiatr. Dis. Treat. 4: 687-699.
PubMed  |  Direct Link  |  

Daiello, L.A., E.K. Festa, B.R. Ott and W.C. Heindel, 2008. Cholinesterase inhibitors improve visual attention in drivers with Alzheimer’s disease. Alzheimer's Dementia: J. Alzheimer's Assoc., 4: T498-T498.
CrossRef  |  Direct Link  |  

Ferreres, F., F. Fernandes, C. Sousa, P. Valentao, J.A. Pereira and P.B. Andrade, 2009. Metabolic and bioactivity insights into Brassica oleracea var. acephala. J. Agric. Food Chem., 57: 8884-8892.
CrossRef  |  Direct Link  |  

Ferri, C.P., M. Prince, C. Brayne, H. Brodaty and L. Fratiglioni et al., 2005. Global prevalence of dementia: A delphi consensus study. Lancet, 366: 2112-2117.
CrossRef  |  PubMed  |  Direct Link  |  

Forbes, D., E.J. Thiessen, C.M. Blake, S.C. Forbes and S. Forbes, 2013. Exercise programs for people with dementia. Cochrane Database Syst. Rev. 10.1002/14651858.CD006489.pub3

He, L., H.T. Li, S.W. Guo, L.F. Liu, J.B. Qiu, F. Li and B.C. Cai, 2008. Inhibitory effects of sinapine on activity of acetylcholinesterase in cerebral homogenate and blood serum of rats. China J. Chin. Mater. Med., 33: 813-815.
Direct Link  |  

Henriksen, E.J., M.K. Diamond-Stanic and E.M. Marchionne, 2010. Oxidative stress and the etiology of insulin resistance and type 2 diabetes. Free Radical Biol. Med., 51: 993-999.
CrossRef  |  Direct Link  |  

Kakkar, S. and S. Bais, 2014. A review on protocatechuic acid and its pharmacological potential. ISRN Pharmacol. 10.1155/2014/952943

Kangtao, Yangqian and S. Bais, 2018. Neuroprotective effect of protocatechuic acid through MAO-B inhibition in aluminium chloride induced dementia of Alzheimer's type in rats. Int. J. Pharmacol., 14: 879-888.
CrossRef  |  Direct Link  |  

Karakida, F., Y. Ikeya, M. Tsunakawa, T. Yamaguchi, Y. Ikarashi, S. Takeda and M. Aburada, 2007. Cerebral protective and cognition-improving effects of sinapic acid in rodents. Biol. Pharm. Bull., 30: 514-519.
CrossRef  |  Direct Link  |  

Kaur, A. and K.D. Gill, 2006. Possible peripheral markers for chronic aluminium toxicity in Wistar rats. Toxicol. Ind. Health, 22: 39-46.
CrossRef  |  PubMed  |  Direct Link  |  

Liu, Y., G. Fiskum and D. Schubert. 2002. Generation of reactive oxygen species by the mitochondrial electron transport chain. J. Neurochem., 80: 780-787.
CrossRef  |  Direct Link  |  

Luck, H., 1971. Catalase. In: Methods of Enzymatic Analysis, Bergmeyer, H.U. (Ed.). Academic Press, New York, pp: 885-893.

Lyle, N., D. Bhattacharyya, T.K. Sur, S. Munsi, S. Paul, S. Chatterjee and A. Gomes, 2009. Stress modulating antioxidant effect of Nardostachys jatamansi. Indian J. Biochem. Biophys., 46: 93-98.
Direct Link  |  

Miska-Schramm, A., J. Kapusta and M. Kruczek, 2017. The effect of aluminum exposure on reproductive ability in the Bank Vole (Myodes glareolus). Biol. Trace Element Res., 177: 97-106.
CrossRef  |  Direct Link  |  

Morris, R., 1984. Developments of a water-maze procedure for studying spatial learning in the rat. J. Neurosci. Methods, 11: 47-60.
CrossRef  |  PubMed  |  Direct Link  |  

Nampoothiri, M., J. John, N. Kumar, J. Mudgal, G.K. Nampurath and M.R. Chamallamudi, 2015. Modulatory role of simvastatin against aluminium chloride-induced behavioural and biochemical changes in rats. Behav. Neurol., Vol. 2015. 10.1155/2015/210169

Nascimento, E.C.M. and J.B.L. Martins, 2011. Electronic structure and PCA analysis of covalent and non-covalent acetylcholinesterase inhibitors. J. Mol. Model., 17: 1371-1379.
CrossRef  |  Direct Link  |  

Niciforovic, N. and H. Abramovic, 2014. Sinapic acid and its derivatives: Natural sources and bioactivity. Compr. Rev. Food Sci. Food Saf., 13: 34-51.
CrossRef  |  Direct Link  |  

Ofen, N., 2012. The development of neural correlates for memory formation. Neurosci. Biobehav. Rev., 36: 1708-1717.
CrossRef  |  Direct Link  |  

Ohkawa, H., N. Ohishi and K. Yagi, 1979. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem., 95: 351-358.
CrossRef  |  PubMed  |  Direct Link  |  

Owokotomo, I.A., O. Ekundayo, T.G. Abayomi and A.V. Chukwuka, 2015. In-vitro anti-cholinesterase activity of essential oil from four tropical medicinal plants. Toxicol. Rep., 2: 850-857.
CrossRef  |  Direct Link  |  

Percy, M.E., T.P. Kruck, A.I. Pogue and W.J. Lukiw, 2011. Towards the prevention of potential aluminum toxic effects and an effective treatment for Alzheimer's disease. J. Inorganic Biochem., 105: 1505-1512.
CrossRef  |  Direct Link  |  

Raskind, M., V. Kumar, L. Malaty, J. Messina, R. Hartman and R. Anand, 2000. Rivastigmine for Alzheimer's disease: Improvement versus reduced worsening. Prim. Care Companion. J. Clin. Psychiatry, 2: 134-138.
PubMed  |  Direct Link  |  

Robbins, R.J., 2003. Phenolic acids in foods:  An overview of analytical methodology. J. Agric. Food Chem., 51: 2866-2887.
CrossRef  |  PubMed  |  Direct Link  |  

Sargazi, M., A. Shenkin and N.B. Roberts, 2006. Aluminium-induced injury to kidney proximal tubular cells: Effects on markers of oxidative damage. J. Trace Elem. Med. Biol., 19: 267-273.
CrossRef  |  PubMed  |  Direct Link  |  

Sherer, T.B., J.R. Richardson, C.M. Testa, B.B. Seo and A.V. Panov et al., 2007. Mechanism of toxicity of pesticides acting at complex I: Relevance to environmental etiologies of Parkinson’s disease. J. Neurochem., 100: 1469-1479.
CrossRef  |  Direct Link  |  

Terry, A.V.Jr. and J.J. Buccafusco, 2003. The cholinergic hypothesis of age and Alzheimer's disease-related cognitive deficits: Recent challenges and their implications for novel drug development. J. Pharmacol. Exp. Ther., 306: 821-827.
CrossRef  |  PubMed  |  Direct Link  |  

WHO., 1992. International classification of diseases 10th revision. World Health Organization, Geneva.

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