• [email protected]
  • +971 507 888 742
Submit Manuscript
SciAlert
  • Home
  • Journals
  • Information
    • For Authors
    • For Referees
    • For Librarian
    • For Societies
  • Contact
  1. Research Journal of Phytochemistry
  2. Vol 9 (2), 2015
  3. 41-55
  • Online First
  • Current Issue
  • Previous Issues
  • More Information
    Aims and Scope Editorial Board Guide to Authors Article Processing Charges
    Submit a Manuscript

Research Journal of Phytochemistry

Year: 2015 | Volume: 9 | Issue: 2 | Page No.: 41-55

Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Article Trend



Total views 251

Authors


Souravh Bais

Country: India

Yash Prashar

Country: India

Keywords


  • leucocytes
  • erythrocytes
  • H2O2
  • Reactive oxygen species
  • Juniperus species
Research Article

Identification and Characterization of Amentoflavone from Six Species of Juniperus Against H2O2 Induced Oxidative Damage in Human Erythrocytes and Leucocytes

Souravh Bais and Yash Prashar
The aim of the present study was to investigate the protective effects of amentoflavone (AF), isolated from six species of Juniperus (AF1-AF6) (Cupressaceae) Juniperus communis L. (AF1), Juniperus wallichiana L. (AF2), Juniperus indica L. (AF3), Juniperus macropoda L. (AF4), juniperus recurva L. (AF5) and Juniperus polycarpos L. (AF6)] against H2O2 induced oxidative damage in human erythrocytes and leucocytes. The experiment was set and designed as per method available in literature. Amentoflavone was isolated from aerial parts of Juniperus species (above six) and authenticated by TLC, IR, HPLC and physicochemical parameters. The two concentration of isolated AF (A = 250 μL, B = 500 μL) were used to assessed its protective effects against H2O2 induced oxidative damage in human erythrocytes and leucocytes. The measurements of different antioxidant parameters like, CAT, SOD and GPx, LPO and GSH levels were performed under each set extracts. Juniperus communis L. (AF1B) (500 μL) and Juniperus indica L. (AF3B) (500 μL) were the most significant one on CAT (28.35±2.980 and 27.73±1.580 AU g–1 Hb), GPx (128.53±3.10 and 41.7±1.420 μg g–1 Hb) and SOD (1193.5±19.7 and 1532.6±17.60 U g–1 Hb) enzyme systems of erythrocytes. Among plant extracts, Juniperus communis L. (AF1B) showed the highest activities on CAT (13.79±1.832 AU mg–1 protein) and SOD (820±14.50 U mg–1 protein) while Juniperus indica L. (AF3B) showed significant effects on Gpx (152.72±3.70 U mg–1 protein), GSH (25.93±1.560 μg mg–1 protein) and LPO (10.54±2.90 nmol mg–1 protein) enzyme systems of leucocytes. The study concludes that isolated fractions of AF from Juniperus species (among six species), has a potential source of natural antioxidants for treatment and prevention of diseases in which oxidative stress takes place.
PDF Fulltext XML References Citation

How to cite this article

Souravh Bais and Yash Prashar, 2015. Identification and Characterization of Amentoflavone from Six Species of Juniperus Against H2O2 Induced Oxidative Damage in Human Erythrocytes and Leucocytes. Research Journal of Phytochemistry, 9: 41-55.

URL: https://scialert.net/abstract/?doi=rjphyto.2015.41.55

Related Articles

Protective Effect of Byrsonima sericea Extract on Non-alcoholic Fatty Liver Disease Model in Rats
Modulation of Pro-inflammatory Mediators by Eugenol in AlCl3 Induced Dementia in Rats
Anti-amnesic Effect of Leea indica Extract in Scopolamine-induced Amnesia of Alzheimer’s Type in Rats
Review on Chemistry and Pharmacological Potential of Amentoflavone
A Review on Current Strategies and Future Perspective in Respect to Alzheimer’s Disease Treatment

Leave a Comment


Your email address will not be published. Required fields are marked *

Useful Links

  • Journals
  • For Authors
  • For Referees
  • For Librarian
  • For Socities

Contact Us

Office Number 1128,
Tamani Arts Building,
Business Bay,
Deira, Dubai, UAE

Phone: +971 507 888 742
Email: [email protected]

About Science Alert

Science Alert is a technology platform and service provider for scholarly publishers, helping them to publish and distribute their content online. We provide a range of services, including hosting, design, and digital marketing, as well as analytics and other tools to help publishers understand their audience and optimize their content. Science Alert works with a wide variety of publishers, including academic societies, universities, and commercial publishers.

Follow Us
© Copyright Science Alert. All Rights Reserved