Science Alert
Curve Top
International Journal of Dairy Science
  Year: 2012 | Volume: 7 | Issue: 3 | Page No.: 63-69
DOI: 10.3923/ijds.2012.63.69
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Effect of Bacterial Interactions on Antimicrobial Compound Production by Lactic Acid Bacteria

R.M.U.S.K. Rathnayaka

In the present study interaction between three lactic acid bacteria species and three food borne pathogenic bacteria species were studied. The objective of the study was to determine the effect of those interactions to the antimicrobial compound producing ability of lactic acid bacteria. Using the three lactic acid bacteria namely, Lactococcus lactis, Lactobacillus acidophilus and Lactobacillus casei and three pathogenic bacteria namely, Salmonella enterica, Escherichia coli and Listeria monocytogenes 56 different culture combinations were prepared and grown in a medium invented by de Man, Rogosa and Sharpe (MRS) broth. Then Cell Free Filtrates (CFF) containing antimicrobial compounds were obtained from all those cultures and tested for antimicrobial activity against the three pathogenic microorganisms. All Cell Free Filtrates (CFF) were found to be having antibiotic activity against tested three pathogenic bacteria. Antibiotic activity of Cell Free Filtrates (CFF) obtained from cultures contained both lactic acid bacteria and some pathogenic bacteria were significantly higher than cultures contained only lactic acid bacteria. However, this stimulating effect of pathogenic bacteria on antimicrobial compound production by lactic acid bacteria was different for different pathogenic bacteria. Stimulation by Escherichia coli was not observed and some times Escherichia coli shown to be reducing the antimicrobial compound production. Antibiotic activity was found to be higher in mixed cultures of lactic acid bacteria compared to individual cultures. Results of this study confirm the importance of using mixed cultures and effective stimulant bacteria in antimicrobial compound production by lactic acid bacteria.
PDF Fulltext XML References Citation Report Citation
  •    Microbial Hazards Associated with Fermented Milk (Roub and Mish) Processing in Sudan
  •    Isolation and Identification of Lactic Acid Bacteria from Raw Cow Milk in Khartoum State, Sudan
  •    Effect of Chicory Water Extract and Lactulose Syrup on Growth and Viability of Lactobacillus plantarum, Lactobacillus casei and Lactobacillus rhamnosuss in Skim Milk
  •    Prevalence and Significance of Staphylococcus aureus and Enterobacteriaceae species in Selected Dairy Products and Handlers
  •    A Bacteriocin Production on Soya Nutri Nuggets Extract Medium by Lactococcus lactis Subsp. Lactis CCSUB202
How to cite this article:

R.M.U.S.K. Rathnayaka , 2012. Effect of Bacterial Interactions on Antimicrobial Compound Production by Lactic Acid Bacteria. International Journal of Dairy Science, 7: 63-69.

DOI: 10.3923/ijds.2012.63.69






Curve Bottom