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Research Journal of Phytochemistry

Year: 2011 | Volume: 5 | Issue: 2 | Page No.: 89-97
Research Article

Phytochemical Evaluation and Radical Scavenging Activity of Bauhinia variegata, Saraca asoka and Terminalia arjuna Barks

A.K. Pandey
Tropical Forest Research Institute, Indian Council of Forestry Research and Education, P.O. RFRC, Mandla Road, Jabalpur-482 021, India
Vijayalakshmi Ojha
Tropical Forest Research Institute, Indian Council of Forestry Research and Education, P.O. RFRC, Mandla Road, Jabalpur-482 021, India
Swati Yadav
Tropical Forest Research Institute, Indian Council of Forestry Research and Education, P.O. RFRC, Mandla Road, Jabalpur-482 021, India
Sonu Kumar Sahu
Tropical Forest Research Institute, Indian Council of Forestry Research and Education, P.O. RFRC, Mandla Road, Jabalpur-482 021, India
Phytochemical estimation and radical scavenging activity were carried out in the barks of Bauhinia variegata, Saraca asoka and Terminalia arjuna collected from central India with the objective to determine optimum harvesting age (girth class). Bark samples were analyzed for total phenols, flavonoids and tannins. Radical scavenging activity of the barks was evaluated using DPPH assay. Significant variation was observed in total phenols and tannins in B. variegata; flavonoids and tannins in T. arjuna with respect to different girth classes. However, no significant variation was found in chemical constituents among different girth classes of S. asoka. Gallic acid was used as standard having IC50 value of 1.06±0.10 μg mL-1. Radical scavenging activity showed significant variation in the barks of different girth classes of B. variegata, S. asoka and T. arjuna. The results revealed that the optimum girth class to harvest barks of B. variegata was 36-55 cm, S. asoka 61-90 cm and T. arjuna 121-150 cm as these were found to contain maximum concentration of active ingredients and possess high radical scavenging activity. Among the studied species T. arjuna showed maximum radical scavenging activity and thus can be termed as a potent antioxidant species whose bark can be used for making various formulations containing natural antioxidants.
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References

  1. Adesegun, S.A., A. Fajana, C.I. Orabueze and H.A.B. Cooker, 2007. Evaluation of antioxidant properties of Phaulopsis fascisepala C.BC.I. (Acanthaceae). Evid. Based Complement. Altern. Med., 4: 1-5.
  2. Afolayan, A.J., O.M. Aboyade and M.O. Sofidiya, 2008. Total phenolic content and free radical scavenging activity of Malva parviflora L. (Malvaceae). J. Biol. Sci., 8: 945-949.
    CrossRefDirect Link
  3. Alpana, R., P. Laurai, R. Gupta, P. Kumar and V.N. Sharma, 1997. Hypocholesterolemic effects of Terminala arjuna tree bark. J. Ethnopharmacol., 55: 65-67.
  4. Balajirao, N.S., D. Rajasekhar, R. Narayana and D. Raju, 1995. Ethno-medical therapy among the Chenchus of Nallamalai forest of Andhra Pradesh. Biosci. Res. Bull., 11: 81-85.
  5. Bele, A.A., V.M. Jadhav and V.J. Kadam, 2010. Potential of tannins: A review. Asian J. Plant Sci., 9: 209-214.
    CrossRef
  6. Berrocal, A., J. Baeza, J. Rodriguez, M. Espinosa and J. Freer, 2004. Effect of tree age on variation of Pinus radiata D. Don chemical composition. J. Chil. Chem. Soc., 49: 251-256.
    CrossRef
  7. Chang, C.C., M.H. Yang, H.M. Wen and J.C. Chern, 2002. Estimation of total flavonoid content in propolis by two complementary colometric methods. J. Food Drug Anal., Vol. 10.
    CrossRefDirect Link
  8. Chaudhari, M. and S. Mengi, 2006. Evaluation of phytoconstituents of Terminalia arjuna for wound healing activity in rats. Phytother. Res., 20: 799-805.
    CrossRefPubMedDirect Link
  9. Cheynier, V., 2005. Polyphenols in foods are more complex than often thought. Am. J. Clin. Nutr., 81: 223S-229S.
    CrossRefPubMedDirect Link
  10. Daniel, M., 2006. Medicinal Plants: Chemistry and Properties. Science Publishers, USA., pp: 1-9.
  11. Dwivedi, S., 1996. Putative uses of Indian cardiovascular friendly plants in preventive cardiology. Ann. Natl Acad. Med. Sci., 32: 159-175.
    Direct Link
  12. Gil, M.I., F. Ferreres and F.A. Thomas-Barberan, 1999. Effect of postharvest storage and processing on the antioxidant constituents (Flavonoids and Vitamin C) of fresh cut spinach. J. Agric. Food Chem., 47: 2213-2217.
    PubMed
  13. Goncalves, C., T. Dinis and M.T. Batista, 2005. Antioxidant properties of proanthocyanidins of Uncaria tomentosa bark decoction: A mechanism for anti-inflammatory activity. Phytochemistry, 66: 89-98.
    CrossRefDirect Link
  14. Gupta, V.K., R. Kumria, M. Garg and M. Gupta, 2010. Recent updates on free radicals scavenging flavonoids: An overview. Asian J. Plant Sci., 9: 108-117.
    CrossRefDirect Link
  15. Kaur, K., S. Arora, S. Kumar and A. Nagpala, 2002. Antimutagenic activities of acetone and methanol fractions of Terminalia arjuna. Food Chem. Toxicol., 40: 1475-1482.
    Direct Link
  16. Khlebnikov, A.I., I.A. Schepetkin, N.G. Domina, L.N. Kirpotina and M.T. Quinn, 2007. Improved quantitative structure-activity relationship models to predict antioxidant activity of flavonoids in chemical, enzymatic and cellular systems. Bioorg. Med. Chem., 15: 1749-1770.
    Direct Link
  17. Koleva, I.I., T.A. van Beek, J.P.H. Linssen, A. de Groot and L.N. Evstatieva, 2002. Screening of plant extracts for antioxidant activity: A comparative study on three testing methods. Phytochem. Anal., 13: 8-17.
    CrossRefDirect Link
  18. Kurien, J.C., 2001. Plants that Heal. 4th Edn., Orient Watchman Publishing House, Pune, India.
  19. McDonald, S., P.D. Prenzler, M. Antolovich and K. Robards, 2001. Phenolic content and antioxidant activity of olive extracts. Food Chem., 73: 73-84.
    CrossRefDirect Link
  20. Nadeem, M., H.C. Rikhari, A. Kumar, L.M.S. Palni and S.K. Nandi, 2002. Taxol content in the bark of Himalayan Yew in relation to tree age and sex. Phytochemistry, 60: 627-631.
    PubMedDirect Link
  21. Nadkarni, A.K. and K.M. Nadkarni, 2005. Indian Materia Medica. Vol. 1, Popular Prakashan Pvt. Ltd., Bombay, India, pp: 403-405.
  22. Nickavar, B., M. Kamalinejad, M. Haj-Yahya and B. Shafaghi, 2006. Comparison of the free radical scavenging activity of six Iranian Achillea species. Pharm. Biol., 44: 208-212.
    Direct Link
  23. Oboh, G., 2008. Antioxidative potential of Ocimum gratissimum and Ocimum canum leaf polyphenols and protective effects on some pro-oxidants induced lipid peroxidation in rat brain: An in vitro study. Am. J. Food Technol., 3: 325-334.
    CrossRefDirect Link
  24. Osawa, T., 1994. Novel Natural Antioxidants for Utilization in Food and Biological Systems. In: Post Harvest Biochemistry of Plant Food Materials in Tropics, Uritani, L., V.V. Garcia and E.M. Mendoza (Eds.). Japan Scientific Societies Press, Tokyo, Japan, pp: 241-251.
  25. Palav, Y.K. and P.M. Dmello, 2006. Standardization of selected Indian medicinal herbal raw materials containing polyphenols as major phytoconstituents. Indian J. Pharm. Sci., 68: 506-509.
    Direct Link
  26. Pandey, A.K. and D.C. Kori, 2009. Variations in tannin and oxalic acid content in Terminalia arjuna (Arjuna) bark. Pharmacogn. Mag., 5: 159-164.
    Direct Link
  27. Parekh, J. and S. Chanda, 2007. Antibacterial and phytochemical studies on twelve species of Indian medicinal plants. Afr. J. Biomed. Res., 10: 175-181.
    CrossRefDirect Link
  28. Patnaik, T., R.K. Dey and P. Gouda, 2007. Isolation of triterpenoid glycoside from bark of Terminalia arjuna using chromatographic technique and investigation of pharmacological behavior upon muscle tissues. E-J. Chem., 4: 474-479.
  29. Rice-Evans, C.A., N.J. Miller, P.G. Bolwell, P.M. Bramley and J.B. Pridham, 1995. The relative antioxidant activity of plant derived polyphenolic flavonoids. Free Radic. Res., 22: 375-383.
    PubMed
  30. Sadasivam, S. and A. Manikam, 1996. Biological Methods. New Age International Pvt. Ltd., New Delhi, pp: 189-191.
  31. Sadhu, S.K., A. Khatun, P. Phattanawasin, T. Ohtsuki and M. Ishibash, 2007. Lignan glycosides and flavonoids from Saraca asoca with antioxidant activity. J. Nat. Med., 61: 480-482.
    CrossRef
  32. Sati, S.C., N. Sati, U. Rawat and O.P. Sati, 2010. Medicinal plants as a source of antioxidants. Res. J. Phytochem., 4: 213-224.
    CrossRefDirect Link
  33. Satyavati, G.V., D.N. Prasad, S.P. Sen and P.K. Das, 1970. Further studies on the uterine activity of Saraca indica Linn. Indian J. Med. Res., 58: 947-960.
    PubMed
  34. Singh, P.B. and B.S. Aswal, 1992. Medicinal plants of Himachal Pradesh used in Indian pharmaceutical industry. Bull. Med. Ethnobot. Res., 13: 172-208.
  35. Subasini, U., G.V. Rajamanickam, G.P. Dubey, P.C. Prabu and C.S. Sahayam et al., 2007. Hydroalcoholic extract of Terminalia arjuna: A potential hepatoprotective herb. J. Boil. Sci., 7: 255-262.
    CrossRefDirect Link
  36. Takahashi, S., H. Tanaka, Y. Hano, K. Ito, T. Nombra and K. Shigenobu, 1997. Hypotensive effects in rats of hydrophyllic extract from Terminalia arjuna containing tannin-related compounds. Phytother. Res., 1: 424-427.
    CrossRef
  37. Thakur, M.J., I.N. Misra and B.K. Chaudhary, 1992. Ethnobotanical studies of some plants of Madhubani district, Bihar (Part-I). J. Econ. Tax. Bot., 16: 383-390.
  38. Tsimogiannis, D.I. and V. Oreopoulou, 2004. Free radical scavenging and antioxidant activity of 5,7,3',4'-hydroxy-substituted flavonoids. Innovative Food Sci. Emerg. Technol., 5: 523-528.
    CrossRef
  39. Yadava, R.N. and V.M. Reddy, 2001. A new flavone glycoside, 5-hydroxy 7,3',4'5'-tetra-methoxy flavone 5-O-β-D-xylopyranosyl-(1->2)-α-L-rhamnopyranoside from Bauhinia variegata Linn. J. Asian Nat. Prod. Res., 3: 341-346.
  40. Vileges, J.H., E. De Marchi and E.M. Lancas, 1997. Phytochemical studies of some medicinal plants. Anal., 8: 266-270.
  41. Vinson, J.A., Y.A. Dabbagh, M.M. Serry and J. Jang, 1995. Plant flavonoids, especially tea flavonols, are powerful antioxidants using an in vitro oxidation model for heart disease. J. Agric. Food. Chem., 43: 2800-2802.
    CrossRefDirect Link
  42. Youdim, K.A., B. Shukitt-Hale and J.A. Joseph, 2004. Flavonoids and the brain: Interaction at the blood-brain barrier and their physiological effects on the central nervous system. Free Radic. Biol. Med., 37: 1683-1693.
    PubMed
  43. Zhao, Y.Y., C.B. Cui, B. Cai, B. Han and Q.S. Sun, 2005. A new phenanthraquinone from the stems of Bauhinia variegata L. J. Asian Nat. Prod. Res., 7: 835-838.
    PubMed
  44. Paarakh, P.M., 2010. Terminalia arjuna (Roxb) wt. and arn.: A review. Int. J. Pharmacol., 6: 515-534.
    CrossRefDirect Link
  45. Arts, I.C.W. and P.C.H. Hollman, 2005. Polyphenols and disease risk in epidemiologic studies. Am. J. Clin. Nutr., 81: 317S-325S.
    PubMedDirect Link

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