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International Journal of Dairy Science
  Year: 2009 | Volume: 4 | Issue: 3 | Page No.: 100-108
DOI: 10.3923/ijds.2009.100.108
Prevalence and Significance of Staphylococcus aureus and Enterobacteriaceae species in Selected Dairy Products and Handlers
H.M. Fadel and Jehan Ismail

Abstract:
Some of the Egyptian dairy products are made by traditional methods. This makes them suitable media for multiplication of foodborne pathogens. So, this study aims to assess the microbial quality of selected dairy products prevalent in Ismailia City, the personal hygiene of dairy handlers and the potential hazards to consumers. A total of 120 Ras cheese, Kareish cheese and ice cream samples were collected randomly from different localities in Ismailia city. In addition, 40 nasal and hand swab samples were collected from some dairy workers. All samples were examined for presence of Staphylococcus aureus (on Baird Parker agar medium) and Enterobacteriaceae sp. (on Violet Red Bile Glucose agar medium). Molecular typing of S. aureus was performed using PCR Assay. The prevalence and significance of Staphylococcus aureus and Enterobacteriaceae species in Ras cheese, Kareish cheese and ice cream samples and in swabs of dairy handlers in Ismailia city were studied. The results of this study revealed out that the mean values of S. aureus counts (log10 cfu g-1) in Ras cheese was 5.54, in Kareish cheese was 5.59 and in ice cream samples was 4.07. Meanwhile, the mean values of Enterobacteriaceae sp. counts (log10 cfu g-1) in Ras cheese was 2.48, in Kareish cheese was 6.78 and in ice cream was 1.11. Salmonella could not be recovered in the examined dairy products. Staphylococcus aureus was isolated from 60 and 70% of dairy handlers hand's and nasal swab samples, respectively. Only one Salmonella strain was recovered from a dairy handler swab. Overall, the recovered E. coli serotypes from all the examined samples were O15:H11, O22:H5, O25: NM, O26:H11, O86:H34, O91:H10, O113:H21, O114:H2, O119:H6, O124:H7, O128:H2, O127:NM and O145:NM. In conclusion, the isolated serotypes constitute public health hazards to consumers.
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How to cite this article:

H.M. Fadel and Jehan Ismail, 2009. Prevalence and Significance of Staphylococcus aureus and Enterobacteriaceae species in Selected Dairy Products and Handlers. International Journal of Dairy Science, 4: 100-108.

DOI: 10.3923/ijds.2009.100.108

URL: https://scialert.net/abstract/?doi=ijds.2009.100.108

COMMENTS
10 November, 2009
gaikwad sunil manikrao:
this is very good research work and is very essential work done by the author(s). best wishes for future investigatiion
 
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