Ronald Tarigan
Department of Anatomy, Physiology and Pharmacology, Faculty of Veterinary Medicine, Bogor Agriculture University, Bogor 16680, Indonesia
Nastiti Kusumorini
Department of Anatomy, Physiology and Pharmacology, Faculty of Veterinary Medicine, Bogor Agriculture University, Bogor 16680, Indonesia
Wasmen Manalu
Department of Anatomy, Physiology and Pharmacology, Faculty of Veterinary Medicine, Bogor Agriculture University, Bogor 16680, Indonesia
ABSTRACT
Obesity has been a world-wide health problem and associated with many degenerative diseases. Two approved anti-obesity compounds, sibutramine and orlistat, have various serious side effects which limit their uses. IgY anti lipase will inhibit the activity of pancreatic lipase in the gastrointestinal tract. The objective of this study was to evaluate the effectivity of IgY anti lipase obtained from yolk as pancreatic lipase inhibitor for prevention of obesity. IgY anti lipase was obtained from the yolk of hens immunized with porcine pancreatic lipase and was purified with sodium sulphate precipitation. The protein was analyzed by SDS-PAGE and was identified by Immunoblotting. The ability of IgY anti lipase to inhibit pancreatic lipase was tested by ELISA colorimetric microplate assay at five grades of concentration. Yolk containing IgY anti lipase was then tested in rabbit to evaluate its ability to inhibit fat absorption in vivo. The result showed that IgY contained two proteins with molecular weights of 61.2 and 26.9 kDa and was positively reacted with the rabbit anti chicken coupled with HRP. IgY anti lipase and orlistat did not differ in inhibition capacity at various concentrations (p>0.05). The inhibition capacity of IgY obtained from unimmunized hen was significantly lower than IgY anti lipase and orlistat (p<0.05). Yolk containing IgY anti lipase also had the ability to inhibit dietary fat absorption in vivo, but its inhibition capacity was lower than orlistat (p<0.05). It is concluded that IgY anti lipase could be used to inhibit fat absorption to prevent obesity.
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How to cite this article
Ronald Tarigan, Nastiti Kusumorini and Wasmen Manalu, 2016. Effectivity of Immunoglobulin Y Anti Lipase as a Pancreatic Lipase Inhibitor for Prevention of Obesity*. Pakistan Journal of Nutrition, 15: 625-632.
DOI: 10.3923/pjn.2016.625.632
URL: https://scialert.net/abstract/?doi=pjn.2016.625.632
DOI: 10.3923/pjn.2016.625.632
URL: https://scialert.net/abstract/?doi=pjn.2016.625.632
REFERENCES
- Akita, E.M. and S. Nakai, 1992. Immunoglobulins from egg yolk: Isolation and purification. J. Food Sci., 57: 629-634.
CrossRefDirect Link - Badmaev, V., Y. Hatakeyama, N. Yamazaki, A. Noro, F. Mohamed, C.T. Ho and M.H. Pan, 2015. Preclinical and clinical effects of Coleus forskohlii, Salacia reticulata and Sesamum indicum modifying pancreatic lipase inhibition in vitro and reducing total body fat. J. Funct. Foods, 15: 44-51.
CrossRefDirect Link - Birari, R.B. and K.K. Bhutani, 2007. Pancreatic lipase inhibitors from natural sources: Unexplored potential. Drug Discovery Today, 12: 879-889.
CrossRef - Blum, P.M., D.L. Phelps, B.J. Ank, H.J. Krantman and E.R. Stiehm, 1981. Survival of oral human immune serum globulin in the gastrointestinal tract of low birth weight infants. Pediatr. Res., 15: 1256-1260.
CrossRefDirect Link - Carriere, F., P. Grandval, P.C. Gregory, C. Renou, F. Henniges, S. Sander-Struckmeier and R. Laugier, 2005. Does the pancreas really produce much more lipase than required for fat digestion? JOP J. Pancreas, 6: 206-215.
PubMedDirect Link - Choi, S.J., J.M. Hwang and S.I. Kim, 2003. A colorimetric microplate assay method for high throughput analysis of lipase activity. J. Biochem. Mol. Biol., 36: 417-420.
PubMed - Choudhary, M. and K. Grover, 2012. Development of functional food products in relation to obesity. Funct. Foods Health Dis., 2: 188-197.
Direct Link - De Caro, J., M. Boudouard, J. Bonicel, A. Guidoni, P. Desnuelle and M. Rovery, 1981. Porcine pancreatic lipase. Completion of the primary structure. Biochimica et Biophysica Acta (BBA)-Protein Struct., 671: 129-138.
CrossRefDirect Link - De Simone, G. and G. D'Addeo, 2008. Sibutramine: Balancing weight loss benefit and possible cardiovascular risk. Nutr. Metab. Cardiovas. Dis., 18: 334-341.
CrossRefPubMedDirect Link - Dujovne, C.A., J.H. Zavoral, E. Rowe and C.M, Mendel, 2001. Effects of sibutramine on body weight and serum lipids: A double-blind, randomized, placebo-controlled study in 322 overweight and obese patients with dyslipidemia. Am. Heart J., 142: 489-497.
CrossRefDirect Link - Eibl, M.M., H.M. Wolf, H. Furnkranz and A. Rosenkranz, 1988. Prevention of necrotizing enterocolitis in low-birth-weight infants by IgA-IgG feeding. N. Engl. J. Med., 319: 1-7.
CrossRefDirect Link - Aleman-Gomez, J. A., N.S. Colwell, T. Sasser and V.B. Kumar, 1992. Molecular cloning and characterization of rabbit pancreatic triglyceride lipase. Biochem. Biophys. Res. Commun., 188: 964-971.
CrossRefDirect Link - Hadvary, P., W. Sidler, W. Meister, W. Vetter and H. Wolfer, 1991. The lipase inhibitor tetrahydrolipstatin binds covalently to the putative active site serine of pancreatic lipase. J. Biochem., 266: 2021-2027.
Direct Link - Hatta, H., M. Kim and T. Yamamoto, 1990. A novel isolation method for hen egg yolk antibody, IgY. Agric. Biol. Chem., 54: 2531-2535.
CrossRefPubMedDirect Link - Jaradat, Z.W. and R.R. Marquardt, 2000. Studies on the stability of chicken IgY in different sugars, complex carbohydrates and food materials. Food Agric. Immunol., 12: 263-272.
CrossRefDirect Link - Kazemipoor, M., C.W.J.W.M. Radzi, G.A. Cordell and I. Yaze, 2012. Potential of traditional medicinal plants for treating obesity: A review. Proceedings of the 2012 International Conference on Nutrition and Food Science, IPCBEE Vol. 39, July 23-24, 2012, IACSIT Press, Singapore, pp: 1-6.
Direct Link - Laemmli, U.K., 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227: 680-685.
CrossRefDirect Link - Larsson, A., D. Carlander and M. Wilhelmsson, 1998. Antibody response in laying hens with small amounts of antigen. Food Agric. Immunol., 10: 29-36.
CrossRefDirect Link - Li, X., T. Nakano, H.H. Sunwoo, B.H. Paek, H.S. Chae and J.S. Sim, 1998. Effects of egg and yolk weights on yolk antibody (IgY) production in laying chickens. Poult. Sci., 77: 266-270.
CrossRefDirect Link - Mo, E.J., Y.H. Jo, J.Y. Jeong, S.B. Kim, Q. Liu, B.H. Hwang and M.K. Lee, 2016. Pancreatic lipase inhibitory phthalide derivatives from the rhizome of Cnidium officinale. Rec. Nat. Prod., 10: 148-153.
Direct Link - Kovacs-Nolan, J. and Y. Mine, 2004. Avian egg antibodies: Basic and potential applications. Avian Poult. Biol. Rev., 15: 25-46.
CrossRefDirect Link - Padwal, R., S.K. Li and D.C.W. Lau, 2003. Long-term pharmacotherapy for overweight and obesity: A systematic review and meta-analysis of randomized controlled trials. Int. J. Obes., 27: 1437-1446.
CrossRefDirect Link - Ryan, D.E. and W. Levin, 1990. Purification and characterization of hepatic microsomal cytochrome P450. Pharmacol. Ther., 45: 153-239.
PubMed - Rahman, S., S. van Nguyen, F.C. Icatlo Jr., K. Umeda and Y. Kodama, 2013. Oral passive IgY-based immunotherapeutics: A novel solution for prevention and treatment of alimentary tract diseases. Hum. Vaccines Immunotherapeut., 9: 1039-1048.
CrossRefDirect Link - Ramirez, G., M. Zavala, J. Perez and A. Zamilpa, 2012. In vitro screening of medicinal plants used in Mexico as antidiabetics with glucosidase and lipase inhibitory activities. Evidence-Complement. Altern. Med.
CrossRefDirect Link - Rubio, M.A., M. Gargallo, A.I. Millan and B. Moreno, 2007. Drugs in the treatment of obesity: Sibutramine, orlistat and rimonabant. Public Health Nutr., 10: 1200-1205.
CrossRefDirect Link - Tonstad, S., S. Rossner, A. Rissanen and A. Astrup, 2016. Medical management of obesity in Scandinavia 2016. Obes. Med., 1: 38-44.
CrossRefDirect Link - Yun, J.W., 2010. Possible anti-obesity therapeutics from nature-A review. Phytochemistry, 71: 1625-1641.
CrossRefDirect Link - Zheng, C.D., Y.Q. Duan, J.M. Gao and Z.G. Ruan, 2010. Screening for anti-lipase properties of 37 traditional Chinese medicinal herbs. J. Chin. Med. Assoc., 73: 319-324.
CrossRefDirect Link