Attibayeba .
Laboratory of Plant Physiology
Jean-Luc Badila
Laboratory of Plant Physiology
Okiemy-Akeli Marie Genevieve
Laboratory of Biochemistry Faculty of Sciences, Marien Ngouabi University, P.O. Box 69, Brazzaville
ABSTRACT
Cytokinins were extracted from the explants of Cichorium intybus L. roots grown under flowering inductive (long-day and red light), or non inductive conditions (short-day). Flowering was expressed as percent after 8 weeks of their development. Overall, cytokinins levels exhibited biphasic trends with initial increasing concentrations followed by a decrease over time. The highest content of cytokinins was observed in explants exposed to either red light or long-day periods. Measured maximum levels of up to 525 ng.g-1 dry weight were obtained under red light exposure conditions. Cytokinins concentrations measured in materials cultured under long-day conditions were slightly lower by quite similar to those obtained under red-light growth conditions. In contrast, much lower peak values (not exceeding 155 ng.g-1 dry weight) were obtained under short-day experimental conditions. The in vitro development of C. intybus under both long-day periods and exposure to red light resulted in percent flowering of 55.75% and 64.28%, respectively. No flowering was observed under short-day experimental conditions. Overall, our results show that both endogenous content of cytokinins and flowering of C. intybus grown in vitro are dependent on both light quality and length of the photoperiod.
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How to cite this article
Attibayeba ., Jean-Luc Badila and Okiemy-Akeli Marie Genevieve, 2010. Changes in Endogenous Cytokinins and in vitro Photoperiodic Flowering Induction in Cichorium intybus L. Pakistan Journal of Nutrition, 9: 230-234.
DOI: 10.3923/pjn.2010.230.234
URL: https://scialert.net/abstract/?doi=pjn.2010.230.234
DOI: 10.3923/pjn.2010.230.234
URL: https://scialert.net/abstract/?doi=pjn.2010.230.234
REFERENCES
- Baaziz, M., N. Mokhlisse, K. Bendiab, L. Koulla, A. Aouad, H. Hdadou and K. Majourhat, 1996. Peroxidases as Marquers in Date Palm Culture. In: Peroxidases, Biochemistry and Physiology, Obinger, C., U. Burner, R. Ebermann, C. Pen and H. Gr (Eds.). University of Agriculture, Vienna and University of Geneva, Geneva,.
- Badenoch-Jones, J., B.G. Rolfe and D.S. Letham, 1984. Phytohormones, Rhizobium mutants and modulation in legumes. Cytokinins metabolism in effective and ineffective pea root nodules in legumes. Plant Physiol., 74: 239-246.
Direct Link - Badila, P. and P. Paulet, 1986. Esthers hydroxycinnamiques et induction photoperiodique florale in vitro chez Cichorium intybus L. Physiol. Plant., 66: 15-20.
CrossRef - Dyson, W.H. and R.H. Hall, 1972. N6-(Δ2 isopentenyl) adenosine its occurrence as a free nucleoside in a autonomous strain of tobacco tissue. Plant Physiol., 50: 616-621.
Direct Link - El-Kbiach, M.L., A. Lamarti, A. Abdali and A. Badoc, 2002. Culture in vitro des bourgeons axillaires de chene-liege (Quercus suber L.). III. Influence de differents hydrates de carbone. Bull. Soc. Pharm. Bordeaux, 141: 105-116.
Direct Link - Majourhat, K., 2002. Etudes des aspects quantitatifs et qualitatifs des peroxydases et des polyphenoloxydases du palmier dattier (Phoenix dactylifera L.). Contribution a l'etude de la diversite genetique de la palmeraie de Marrakech et des nouvelles plantations. Ph.D. Thesis, Universite Cadi Ayyad, Marrakech (Maroc).
- Miller, C.O., 1975. Revised methods for purification of ribosyl-trans-zeatin from Vincia rosea L. crown gall tumor tissue. Plant Physiol., 55: 448-449.
Direct Link - Palni, L.M.S. and R. Horgan, 1983. Cytokinins biosynthesis in crown gall tissue of Vincia rosea L. Metabolism of isopentenyladenin. Phytochemistry, 22: 1597-1601.
CrossRefDirect Link - Tay, S.A.B. and L.M.S. Palni, 1987. Identification of cytokinin glucoside in a seaweed extract. J. Plant Growth Regul., 5: 133-138.
CrossRefDirect Link - Van Staden, J. and G.G. Dimalla, 1980. Endogenous cytokinins in Bougainvillea San diego red. I: Occurrence of cytokinins glucosides in the root sap. Plant Physiol., 65: 852-854.
Direct Link - Van Staden, J., J.H. Margaret and S.E. Drewes, 1983. Cytokinins in the leaves of Ginkgo biloba. I: The complex in mature leaves. Plant Physiol., 73: 223-227.
Direct Link