G. L. Lima-Filho
Universidade do Estado do Rio de Janeiro, Instituto de Biologia Roberto Alcantara Gomes, Departamento de Bioffsica e Biometria. Av. 28 de setembro, 87, Rio de Janeiro, RJ, 20551-030, Brazil
U. C. Araujo
Universidade do Estado do Rio de Janeiro, Instituto de Biologia Roberto Alcantara Gomes,
Departamento de Biologia Celular e Genetica, Rua Sao Francisco Xavier, 524, Rio de Janeiro, RJ, 20559-900, Brazil
G.M. T. Lima
Universidade Federal de Pernambuco, Hospital das Clfnicas, Departamento de Medicina Tropical. Av.
Prof. Moraes Rego sin, Recife- PE, 50460-390, Brazil
L.C.M. Aleixo
Universidade do Estado do Rio de Janeiro, Instituto de Biologia Roberto Alcantara Gomes, Departamento de Bioffsica e Biometria. Av. 28 de setembro, 87, Rio de Janeiro, RJ, 20551-030, Brazil
S.R. F. Moreno
Universidade do Estado do Rio de Janeiro, Instituto de Biologia Roberto Alcantara Gomes, Departamento de Bioffsica e Biometria. Av. 28 de setembro, 87, Rio de Janeiro, RJ, 20551-030, Brazil
S. D. Santos-Filho
Universidade do Estado do Rio de Janeiro, Instituto de Biologia Roberto Alcantara Gomes, Departamento de Bioffsica e Biometria. Av. 28 de setembro, 87, Rio de Janeiro, RJ, 20551-030, Brazil
R. S. Freitas
Universidade do Estado do Rio de Janeiro, Instituto de Biologia Roberto Alcantara Gomes, Departamento de Bioffsica e Biometria. Av. 28 de setembro, 87, Rio de Janeiro, RJ, 20551-030, Brazil
M. V. Castro-Faria
Universidade do Estado do Rio de Janeiro, Instituto de Biologia Roberto Alcantara Gomes,
Departamento de Biologia Celular e Genetica, Rua Sao Francisco Xavier, 524, Rio de Janeiro, RJ, 20559-900, Brazil
M. Bernardo-Filho
Instituto Nacional do Cancer, Coordenadoria de Pesquisa, Pra9a Cruz Vermelha, 23, Rio de Janeiro, RJ, 20230-130, Brazil
E-mail [email protected]
ABSTRACT
Copper (Cu2+), a component of the mitochondrial electron transport chain which also functions in iron absorption, mobilization and in maintenance of brain neurotransmitter levels, is well known to generate cytotoxic reactive oxygen species (ROS). The aim of this study was to test a sensitive, simple and low cost enzymatic method based on the urease inhibition by very low concentrations of copper and to use this technique to study the protective effect of phytic acid against copper ions. In this way, an enzymatic method based on the ability of urease to be inhibited by very low concentrations of copper ion was utilized. Inhibition curves showed that copper (0.79 and 1.57 μ M) promoted a 43.82 ± 2.72 and 71.84 ± 6.29% inhibition of the enzyme activity, respectively. Enzyme inhibition by the same copper concentrations in the presence of phytic acid (10 mM) were markedly lower. In conclusion, phytic acid has a copper chellating capacity that may be useful in preventing copper interaction with biomolecules and, accordingly, in lowering the generation of ROS.
How to cite this article
G. L. Lima-Filho, U. C. Araujo, G.M. T. Lima, L.C.M. Aleixo, S.R. F. Moreno, S. D. Santos-Filho, R. S. Freitas, M. V. Castro-Faria and M. Bernardo-Filho, 2004. A New in Vitro Enzymatic Method to Evaluate
the Protective Effect of Phytic Acid Against Copper Ions. Pakistan Journal of Nutrition, 3: 118-121.
DOI: 10.3923/pjn.2004.118.121
URL: https://scialert.net/abstract/?doi=pjn.2004.118.121
DOI: 10.3923/pjn.2004.118.121
URL: https://scialert.net/abstract/?doi=pjn.2004.118.121