This study was designed to determine the influence of cell bound phytase supplemented in soybean meal on growth and survival of juvenile milkfish Chanos chanos. Four diets were formulated with different levels of fish meal, soybean meal and phytase. The first diet (control) (F) contains 61.35% of fish meal. The fish meal was replaced by the same level of soybean meal in the second (S), third (SP500) and fourth (SP1000) diets, however, SP500 and SP1000 were supplemented with 500 and 1000 phytase activity units (FTU) kg-1, respectively. The replacement of fish meal by soybean meal, with out phytase supplementation reduced weight gain, specific growth rate and crude protein content in the milkfish. The growth performance in fish fed diet SP1000 was as good as those fed diet F. Results indicated that soybean meal supplemented with 1000 FTU kg-1 phytase can be used for the mass culture of milkfish.
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Phytase, an enzyme chemically known as myo-inositol-hexaphosphate phosphohydrolase (class 3: Hydrolases), is produced either by microorganisms or present in some plant ingredients. Presence of phytase in some animals is of microbial origin. Phytic acid is an abundant plant constituent. It is an organic form of phosphorus, which is chemically a myo-inositol-hexakis-dihydrogen phosphate. The molecular formula of phytic acid is C6H18O24P6 and its molecular weight is 659.86. Salts of phytic acid are called phytates. Most foods of plant origin contain 50 to 80% of their total phosphorus as phytate (Harland and Morris, 1995). Phytic acid interacts with other food ingredients due to which it acts as an antinutritional factor in several ways. Phytates reduce digestibility of proteins, starch and lipids. The amino group present on the side chain of the amino acid is one of the functional groups involved in protein-phytate interaction, thereby decreasing the digestibility of proteins. Phytate complexes with proteins, making them less soluble (Dvorakova, 1998). The salts of phytic acid are known as phytins and their availability and digestibility to monogastric animals including fish is very limited due to lack of intestinal phytase (Pointillart et al., 1987). Soybean products vary in their nutrient and antinutritional factors with regard to different fish species. Further, size related differences in nutrient requirements and tolerance of fish to dietary antinutritional factor is also reported (NRC, 1993). It is noteworthy, however, that these drawbacks can be minimized through the addition of phytase to the diet (Simons et al., 1990; Rodehutscord and Puffer, 1995). Use of phytase in feed reduces or sometimes eliminates the necessity of mineral supplementation, which also decreases the cost of feeds. Although phytase was first used for environmental reasons, it has now been discovered that there are a range of other nutritional and health benefits from using these enzymes. Usage of plant based fish feed in fish culture in lieu of fishmeal might be cost effective. A preliminary study in milk fish C. chanos explored the possibility of replacement of fish meal by soybean meal supplemented with phytase. Milkfish was chosen because it is one of the most ideal fish for farming in Indian coastal waters. Further it is commercially important, show faster growth rate in the first year of life and wide range of tolerance to temperature, dissolved oxygen and salinity. The aim of this study was therefore to determine the effect of phytase supplementation required in plant based diets to improve growth performance and retention efficiencies of nutrients and energy by the milk fish C. chanos.
MATERIALS AND METHODS
Composition and chemical analyses of the experimental diets are shown in Table 1. Four diets were formulated with different levels of fish meal, soybean meal and phytase. The first diet (control) (F) contains 61.35% of fish meal. The second (S), third (SP500) and fourth (SP1000) diets contain 61.35% of soybean meal and the diets third and fourth were supplemented with 500 and 1000 phytase activity units (FTU) kg-1, respectively. The diets were made into pellets of 3mm diameter by a laboratory pellet machine after mixing 100 parts of ingredients with 30 parts of tap water. The diets were freeze dried and stored in a refrigerator at 20oC until used.
|Table 1:||Feed formulation ingredients that constituted the experimental feeds|
Nine hundred forty juvenile milk fish Chanos chanos (mean weight = 9g) were obtained from the Central Institute of Brackish water Aquaculture (CIBA) Chennai, India and were randomly distributed among eight 100 l tanks and two 300 l tanks, where the fish were allowed to acclimate to the new rearing tanks for two weeks. The photoperiod was set at 12 h light: 12 h dark. Tanks were supplied with filtered tap water and the rearing tank was aerated to maintain the oxygen level near 100% saturation. The temperature was maintained at 29±1oC. Fish were fed to apparent satiation with a commercial diet twice a day at 08:00 and 16:00 h during the acclimation period.
After conditioning for two weeks, the fish were starved for 24 h and body weight was measured. The stocking density was 30 fish (mean weight 11.48 g) per tank. The experiment was designed so that duplicate tank of each treatment was randomly assigned, thus reducing tank effect. Fish were fed to apparent satiation with the experimental diets twice per day, at 08:00 and 16:00 h, 7 days per week for three weeks. Individual body weights were measured weekly. Fish were deprived of food for 24 h before each weighing. All possible care was taken during feeding so that no uneaten food settled down on the tank bottom. The fecal matter was removed from the tanks and the tanks were thoroughly cleaned.
The data obtained were analyzed for specific growth rate (SGR), feed conversion ratio (FCR), weight gain (WG) and survival, using the following formula:
|SGR (%)||=||[100 x (In average final weight - In average initial weight) / Number of culture days].|
|FCR||=||Total dry feed intake (g)/wet weight gain (g).|
|WG (%)||=||100 x (Final weight - Initial weight) / Initial weight.|
|Survival||=||100 x (Final number of fishes / Initial number of fishes).|
Growth data (Table 2) show that final body weight, feed intake (FI), feed conversion ratio (FCR) weight gain (WG) and specific growth rate (SGR) were significantly influenced by phytase supplementation. The replacement of fish meal with soybean meal resulted in reduced weight gain and SGR in milk fish C. chanos. However, phytase supplementation in SP1000 FTU kg-1 significantly increased weight gain and SGR in milk fish C. chanos. Feed consumption and FCR were highly depressed, when fish meal was replaced by soybean meal without phytase supplementation. However, both parameters were improved in fish fed diet SP1000 FTU kg-1 (Table 2). In fish fed diet SP1000 weight gain and SGR were as good as for fish fed diet F. However, among the two concentrations of phytase used; SP1000 FTU kg-1 was found ideal in terms of weight gain and SGR for milk fish C. chanos than SP500FTU kg-1.
The proximate body compositions of fish fed on different diets are presented in Table 3. There were no significant differences in moisture, crude fiber, dry matter and ash content among the different treatments. Fish fed diet SP1000 showed a higher whole body crude protein and crude fat content than those fed diets (S) and SP500.
The reduced growth performance and feed consumption in fish fed diet S, where fish meal was replaced by soybean meal without phytase supplementation, confirmed the observations of a preliminary study concerning the effect of fish meal replacement by soybean meal on growth performance and feed consumption in milk fish C. chanos. The finding that fish meal replacement by soybean meal without phytase resulted in lower growth performance parallels the findings in other species (Pongmaneerat and Watanabe, 1992; Rumsey et al., 1994; Stickney et al., 1996; Yoo et al., 2005). It has been demonstrated that dietary fish meal levels can be considerably reduced without any adverse consequence in terms of somatic growth or nutrient utilization using plant based feed with phytase supplementation (Kaushik et al., 1995, 2004; Muzinic et al., 2004). The slight suppression in overall growth performance in fish fed diets SP500 FTU kg-1 might be attributed to the presence of antinutritional factor in soybean meal (Kakade et al., 1973; Vaintraub and Bulmaga, 1991; Linener, 1994) and an adverse effect of phytate on growth performance and bioavailability of various dietary components (Spinelli et al., 1983; Richardson et al., 1985; Satoh et al., 1989).
The phytase supplementation in diet SP1000 (1000 FTU kg-1), however, significantly improved growth performance in milk fish C. chanos compared to the diet with phytase SP500 (500 FTU kg-1) supplementation. This suggests that phytase at 1000 FTU kg-1 diet may be effective at reducing either an antinutritional factor or adverse consequences of phytate from soybean meal (Liu, 1997). The reduction of phytate-protein complexes in the gut and increased nutrient availability could be another explanation for this observation (Liebart and Portz, 2005). The improvement in growth performance after phytase supplementation is consistent with other studies, where fish were fed either phytase supplemented diets (Rodehutscord and Puffer, 1995; Jackson et al., 1996; Papatryphon et al., 1999) or phytase pretreated ingredients (Vielma et al., 2002).
|Table 2:||Growth performance in milk fish C. chanos fed diets with different levels of fish meal, soybean meal and phytase supplementations. Each value is the mean±SE derived from three samples each of 15 juveniles|
|Table 3:||Proximate composition and biological measurements in milk fish C. chanos under different treatments. Each value is mean derived from two samples each of three fish for each treatment|
Similar patterns of phytase activity on growth performance have also been observed in rainbow trout Oncorhynchus mykiss, when canola protein concentrate was used with different dosages of phytase (500, 1500 and 4500 FTU kg-1 diet). Forster et al. (1999) demonstrated a higher SGR in fish fed phytase at 500 FTU kg-1 diet. Phytase dosage higher than 500 FTU kg-1 diet did not further increase the SGR in their observations. In Korean rock fish, although phytase supplementation in soybean meal diets at 1000 and 2000 FTU kg-1 did not increase growth performance, inferior results were observed when phytase was supplemented at 2000 FTU kg-1 diet compared to 1000 FTU kg-1 diet (Yoo et al., 2005).
Dietary phytates may form phytate-protein complexes and reduce the availability of dietary protein and amino acids (Liu et al., 1998; Sugiura et al., 2001). Francis et al. (2001) reviewed antinutritional factors for fish and indicated that growth and feed efficiency in commonly cultured fish species, such as carp (Cyprinus carpio), tilapia (Oreochromis niloticus), trout (Oncorhynchus mykiss) and salmon (Salmo salar), is negatively affected by the inclusion of phytate containing ingredients in the diets. In vitro studies have shown that phytate-protein complexes are insoluble and consequently less digestible by proteolytic enzyme (Ravindran et al., 1995). By virtue of this chelating potential, supplemental microbial phytase may have protein sparing effects in fish diets by releasing phytate bound protein and improving its bioavailability. Plant protein utilization has been reported to increase (Storebakken et al., 1998; Vielma et al., 1998; Sugiura et al., 2001) or to remain unchanged (Lanari et al., 1998; Yan and Reigh, 2002).
Supplementation of microbial phytase in the diets had a positive effect on the growth performance of Labeo rohita juveniles (Baruah et al., 2007). Present study also suggests that culture of milkfish Chanos chanos with soybean meal is possible with phytase supplementation.
The authors are thankful to ICAR, New Delhi for financial assistance through a research project (No: 0623009 F. No: 4 (05) 2004 - ASR - I).
- Baruah, K., N.P. Sahu, A.K. Pal, K.K. Jain, D. Debnath and S.C. Mukherjee, 2007. Dietary microbial phytase and citric acid synergistically enhances nutrient digestibility and growth performance of Labeo rohita (Hamilton) juveniles at sub-optimal protein level. Aquacult. Res., 38: 109-120.
- Forster, I., D.A. Higgs, B.S. Dosanjh, M. Rowshandeli and J. Parr, 1999. Potential for dietary phytase to improve the nutritive value of canola protein concentrate and decrease phosphorus output in rainbow trout (Oncorhynchus mykiss) held in 11°C fresh water. Aquaculture, 179: 109-125.
- Francis, G., H.P.S. Makkar and K. Becker, 2001. Antinutritional factors present in plant-derived alternate fish feed ingredients and their effects in fish. Aquaculture, 199: 197-227.
- Jackson, L.S., M.H. Li and E.H. Robinson, 1996. Use of microbial phytase in channel catfish Ictalurus punctatus diets to improve utilization of phytate phosphorus. World Aquacult. Soc., 27: 309-313.
- Kakade, M.L., D.E. Hoffa and I.E. Liener, 1973. Contribution of trypsin inhibitors to the deleterious effects of unheated soybeans fed to rats. J. Nutr., 103: 1772-1778.
- Kaushik, S.J., J.P. Cravedi, J.P. Lalles, J. Sumpter, B. Fauconneau and M. Laroche, 1995. Partial or total replacement of fish meal by soybean protein on growth, protein utilization, potential estrogenic or antigenic effects, cholesterolemia and flesh quality in rainbow trout, Oncorhynchus mykiss. Aquaculture, 133: 257-274.
- Kaushik, S.J., D. Coves, G. Dutto and D. Blanc, 2004. Almost total replacement of fish meal by plant protein sources in the diet of marine teleost, the European seabass, Dicentrarchus labrax. Aquaculture, 230: 391-404.
- Liebert, F. and L. Portz, 2005. Nutrient utilization of Nile tilapia Oreochromis niloticus fed plant based low phosphorus diets supplemented with graded levels of different sources of microbial phytase. Aquaculture, 248: 111-119.
- Liu, B.L., A. Rafiq, Y.M. Tzeng and A. Rob, 1998. The induction and characterization of phytase and beyond. Enzyme Microbiol. Technol., 22: 415-424.
- Muzinic, L.A., K.R. Thompson, A. Morris, C.D. Webster, D.B. Rouse and L. Manomaitis, 2004. Partial and total replacement of fish meal with soybean and brewer`s grains with yeast in practical diets for Australian red claw crayfish Cherax quadricarinatus. Aquaculture, 230: 359-376.
- Papatryphon, E., R.A. Howell and J.H. Jr. Soares, 1999. Growth and mineral absorption by striped bass Morone saxatilis fed a plant feedstuff based diet supplemented with phytase. J. World Aquacult. Soc., 30: 161-173.
- Pointillart, A., A. Fourdin and N. Fontaine, 1987. Importance of cereal phytase activity for phytate phosphorus utilization by growing pigs fed diets containing triticale or corn. J. Nutr., 113: 907-913.
- Ravindran, V., W.L. Bryden and E.T. Kornegay, 1995. Phytates: Occurrence, bioavailability and implications in poultry nutrition. Poult. Avian Biol. Rev., 6: 125-143.
- Richardson, N.L., D.A. Higgs, R.M. Beames and J.R. McBride, 1985. Influence of dietary calcium, phosphorus, zinc and sodium phytate level on cataract incidence, growth and histopathology in juvenile Chinook salmon (Oncorhynchus tshawytscha). J. Nutr., 115: 553-567.
- Satoh, S., W.E. Poe and R.P. Wilson, 1989. Effect of supplemental phytate and/or tricalcium phosphate on weight gain, feed efficiency and zinc content in vertebrae of channel catfish. Aquaculture, 80: 155-161.
- Simons, P.C.M., H.A.J. Versteegh, A.W. Jongbloed, P.A. Kemme and P. Slump et al., 1990. Improvement of phosphorus availability by microbial phytase in broilers and pigs. Br. J. Nutr., 64: 525-540.
- Spinelli, J., C.R. Houle and J.C. Wekell, 1983. The effect of phytates on the growth of rainbow trout (Salmo gairdneri) fed purified diets containing varying quantities of calcium and magnesium. Aquaculture, 30: 71-83.
- Storebakken, T., K.D. Shearer and A.J. Roem, 1998. Availability of protein, phosphorus and other elements in fish meal, soy-protein concentrate and phytase-treated soy-protein-concentrate-based diets to Atlantic salmon, Salmo salar. Aquaculture, 161: 365-379.
- Sugiura, S.H., J. Gabaudan, F.M. Dong and R.W. Hardy, 2001. Dietary microbial phytase supplementation and the utilization of phosphorus, trace minerals and protein by rainbow trout [Oncorhynchus mykiss (Walbaum)] fed soybean meal-based diets. Aquacult. Res., 32: 583-592.
- Vaintraub, I.A. and V.P. Bulmaga, 1991. Effect of phytate on the in vitro activity of digestive proteinases. J. Agric. Food Chem., 39: 859-861.
- Vielma, J., S.P. Lall, J. Koskela, J. Schoner and P. Mattila, 1998. Effects of dietary phytase and cholecalciferol on phosphorus bioavailability in rainbow trout (Oncorhynchus mykiss). Aquaculture, 163: 309-323.
- Vielma, J., K. Ruohonen and M. Peisker, 2002. Dephytinization of two soy proteins increases phosphorus and protein utilization by rainbow trout, Oncorhynchus mykiss. Aquaculture, 204: 145-156.
- Yan, W., R.C. Reigh and Z. Xu, 2002. Effects of fungal phytase on utilization of dietary protein and minerals and dephosphorylation of phytic acid in the alimentary tract of channel catfish Ictalurus punctatus fed an all-plant-protein diet. J. World Aquac. Soc., 33: 10-22.
- Yoo, G.Y., X. Wang, S. Choi, K. Han, J.C. Kang and S.C. Bai, 2005. Dietary microbial phytase increased the phosphorus digestibility in juvenile Korean rockfish Sebastes schlegeli fed diets containing soybean meal. Aquaculture, 243: 315-322.
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