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Research Article
 

Combined Inorganic Iron (FeSO4) and Steamed Pumpkin (Cucurbita moschata) Fortification in Fresh Noodles Can Increase Hemoglobin Levels in Iron Deficient Mice



Ninik Rustanti, Retno Murwani and Syaiful Anwar
 
ABSTRACT

Noodles are important and popular diet in and outside Asian countries and therefore it is a potential iron carrier food which can provide wide geographical acceptance and coverage. Noodles which had been fortified with inorganic or organic iron only or combined with steamed pumpkin were fed to mice to study its ability to increase Hb level. Thirty 3 weeks old Balb/c mice (Mus musculus) with body weight of 22.9-24.8 g were allocated randomly into five treatment groups: (1) control unfortified wet noodles (unfortified), (2) inorganic iron fortified noodles (FeSO4), (3) organic iron fortified noodles (Bioplex), (4) combined inorganic iron-steam pumpkin fortified noodles (FeSO4+P), (5) combined organic iron-steam pumpkin fortified noodles (Bioplex+P). Each group consisted of six replicate mice. Iron depletion period by feeding unfortified noodles was performed for 14 days followed by treatment noodles for 14 days. The results showed that fortified noodles could increase serum iron levels (p = 0.0001) and transferrin saturation (p = 0.0001) in all groups. However, it could increase Hb levels (p = 0.0001) only in fortified but not unfortified groups. Body weight (p = 0.965), liver weight (p = 0.086) and liver iron levels were not affected by the treatments. Our studies demonstrated that noodles which have been fortified with combined inorganic iron and steamed pumpkin can serve as food carriers to intervene iron deficiency anemia in mice.

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  How to cite this article:

Ninik Rustanti, Retno Murwani and Syaiful Anwar, 2015. Combined Inorganic Iron (FeSO4) and Steamed Pumpkin (Cucurbita moschata) Fortification in Fresh Noodles Can Increase Hemoglobin Levels in Iron Deficient Mice. Pakistan Journal of Nutrition, 14: 316-321.

DOI: 10.3923/pjn.2015.316.321

URL: https://scialert.net/abstract/?doi=pjn.2015.316.321

REFERENCES
Acda, S.P. and B.J. Chae, 2002. A review on the applications of organic trace minerals in pig nutrition. Pak. J. Nutr., 1: 25-30.
CrossRef  |  Direct Link  |  

Allen, L. and J. Casterline-Sabel, 2001. Prevalence and Causes of Nutritional Anemias. In: Nutritional Anemia, Ramakrishnan, U. (Ed.). CRC Press, Boca Raton, pp: 7-21.

Baltussen, R., C. Knai and M. Sharan, 2004. Iron fortification and iron supplementation are cost-effective interventions to reduce iron deficiency in four subregions of the world. J. Nutr., 134: 2678-2684.
PubMed  |  Direct Link  |  

Batra, J. and A. Sood, 2005. Iron deficiency anaemia: Effect on congnitive development in children: A review. Indian J. Clin. Biochem., 20: 119-125.
CrossRef  |  Direct Link  |  

De Bernoist, B., E. McLean, I. Egli and M. Cogswell, 2008. Worldwide Prevalence of Anaemia 1993-2005: WHO Global Database on Anaemia. World Health Organization, Geneva, ISBN: 978-92-4-159665-7, Pages: 40.

Domke, R., B. Groβklaus, H. Niemann, H. Przyrembel and K. Richter et al., 2005. Use of minerals in foods: Toxicological and nutritional-physiological aspects, part II. Federal Institute For Risk Assessment Press, Berlin, Germany. http://www.bfr.bund.de/cm/350/use_of_minerals_in_foods.pdf.

Garcia-Casal, M.N., M. Layrisse, J.P. Pena-Rosas, J. Ramirez, I. Leets and P. Matus, 2003. Iron absorption from elemental iron-fortified corn flakes in humans. Role of vitamins A and C1-3. Nutr. Res., 23: 451-463.
CrossRef  |  Direct Link  |  

Garcia-Casal, M.N., M. Layrisse, L. Solano, M.A. Baron and F. Arguello et al., 1998. Vitamin A and β-carotene can improve nonheme iron absorption from rice, wheat and corn by humans. J. Nutr., 128: 646-650.
Direct Link  |  

Gibson, R.S., 2005. Principles of Nutritional Assessment. 2nd Edn., Oxford University Press, New York, ISBN-13: 9780195171693, Pages: 908.

Grantham-McGregor, S. and C. Ani, 2001. A review of studies on the effect of iron deficiency on cognitive development in children. J. Nutr., 131: 649S-668S.
Direct Link  |  

Hoffbrand, A.V. and J.E. Pettit, 1993. Essential Haematology. 3rd Edn., Blackwell Scientific Publications, London, UK.

Hou, G.G., 2010. Preface. In: Asian Noodles: Science, Technology and Processing, Hou, G.G. (Ed.). John Wiley and Sons Inc., New York, USA., ISBN-13: 9780470634363.

Hurrell, R.F., 2002. Fortification: Overcoming technical and practical barriers. J. Nutr., 132: 806S-812S.
PubMed  |  Direct Link  |  

Joshi, M. and R. Gumashta, 2013. Weekly iron folate supplementation in adolescent girls-an effective nutritional measure for the management of iron deficiency Anaemia. Global J. Health Sci., 5: 188-194.
CrossRef  |  Direct Link  |  

Kusumawati, D., 2004. Friends with Experimental Animals. Gadjah Mada University Press, Yogyakarta, Indonesia.

Layrisse, M., M.N. Garcia-Casal, L. Solano, M.A. Baron and F. Arguello et al., 1997. The role of vitamin A on the inhibitors of nonheme iron absorption: Preliminary results. J. Nutr. Biochem., 8: 61-67.
CrossRef  |  Direct Link  |  

Mehansho, H., 2006. Iron fortification technology development: New approaches. J. Nutr., 136: 1059-1063.
Direct Link  |  

Ministry of Health, 2013. [Basic health research in year 2013]. Report, Ministry of Health, Indonesia. http://www.litbang.depkes.go.id/sites/download/rkd2013/Laporan_Riskesdas2013.PDF, (In Indonesian).

Murwani, R., 2008. Feed Additives: Natural Additives of Antibiotic Substitute. UNNES Press, Semarang, Indonesia.

Roodenburg, A.J.C., C.E. West, R. Hovenier and A.C. Beynen, 1996. Supplemental vitamin a enhances the recovery from iron deficiency in rats with chronic vitamin a deficiency. Br. J. Nutr., 75: 623-636.
CrossRef  |  Direct Link  |  

Rucker, R. and D. Storms, 2002. Interspecies comparisons of micronutrient requirements: Metabolic vs. Absolute body size. J. Nutr., 132: 2999-3000.
Direct Link  |  

Rustanti, N., R. Murwani and S. Anwar, 2011. Acceptability and nutrient content of wet noodles fortified with inorganic or organic iron and provitamin a from pumpkin (Cucurbita moschata). Media Medika Indonesiana, 45: 194-199.
Direct Link  |  

Setyani, S., S.U. Nurdin, E. Arafat and A. Iriyanto, 2004. [Bioavailability and efficiency of ferrous sulfate and citrate in vivo of iron fortified milk candy]. Prosiding Seminar Nasional dan Kongres Perhimpunan Ahli Teknologi Pangan Indonesia (PATPI), December 17-18, 2004, Jakarta, Indonesia, pp: 596-600, (In Indonesian).

Subagio, H.W., 2007. Some thoughts for the Improvement of Anemia in Pregnancy Prevention Program. Agency Publisher of Diponegoro University, Semarang.

USDA., 2011. USDA national nutrient database for standard reference, release 24. Nutrient Data Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, USA. http://www.ars.usda.gov/Services/docs.htm?docid=8964.

Widowati, S., L. Sukarno, Suarni and O. Komalasari, 2003. [Pumpkin: Usability and process of making flour]. Prosiding Seminar Nasional and Pertemuan Tahunan Perhimpunan Ahli Teknologi Pangan Indonesia (PATPI), July 22-23, 2003, Yogjakarta, Yogyakarta, Indonesia, (In Indonesian) -.

Zimmermann, M.B., R. Biebinger, F. Rohner, A. Dib, C. Zeder, R.F. Hurrell and N. Chaouki, 2006. Vitamin A supplementation in children with poor vitamin A and iron status increases erythropoietin and hemoglobin concentrations without changing total body iron. Am. J. Clin. Nutr., 84: 580-586.
Direct Link  |  

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