Ninik Rustanti
Master Program in Nutrition, Faculty of Medicine
Retno Murwani
Natural Product Laboratory, Centre of Research and Services, Diponegoro University, Semarang, Indonesia
Syaiful Anwar
Faculty of Animal Science and Agriculture,
ABSTRACT
Noodles are important and popular diet in and outside Asian countries and therefore it is a potential iron carrier food which can provide wide geographical acceptance and coverage. Noodles which had been fortified with inorganic or organic iron only or combined with steamed pumpkin were fed to mice to study its ability to increase Hb level. Thirty 3 weeks old Balb/c mice (Mus musculus) with body weight of 22.9-24.8 g were allocated randomly into five treatment groups: (1) control unfortified wet noodles (unfortified), (2) inorganic iron fortified noodles (FeSO4), (3) organic iron fortified noodles (Bioplex), (4) combined inorganic iron-steam pumpkin fortified noodles (FeSO4+P), (5) combined organic iron-steam pumpkin fortified noodles (Bioplex+P). Each group consisted of six replicate mice. Iron depletion period by feeding unfortified noodles was performed for 14 days followed by treatment noodles for 14 days. The results showed that fortified noodles could increase serum iron levels (p = 0.0001) and transferrin saturation (p = 0.0001) in all groups. However, it could increase Hb levels (p = 0.0001) only in fortified but not unfortified groups. Body weight (p = 0.965), liver weight (p = 0.086) and liver iron levels were not affected by the treatments. Our studies demonstrated that noodles which have been fortified with combined inorganic iron and steamed pumpkin can serve as food carriers to intervene iron deficiency anemia in mice.
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How to cite this article
Ninik Rustanti, Retno Murwani and Syaiful Anwar, 2015. Combined Inorganic Iron (FeSO4) and Steamed Pumpkin (Cucurbita moschata) Fortification in Fresh Noodles Can Increase Hemoglobin Levels in Iron Deficient Mice. Pakistan Journal of Nutrition, 14: 316-321.
DOI: 10.3923/pjn.2015.316.321
URL: https://scialert.net/abstract/?doi=pjn.2015.316.321
DOI: 10.3923/pjn.2015.316.321
URL: https://scialert.net/abstract/?doi=pjn.2015.316.321
REFERENCES
- Acda, S.P. and B.J. Chae, 2002. A review on the applications of organic trace minerals in pig nutrition. Pak. J. Nutr., 1: 25-30.
CrossRefDirect Link - Baltussen, R., C. Knai and M. Sharan, 2004. Iron fortification and iron supplementation are cost-effective interventions to reduce iron deficiency in four subregions of the world. J. Nutr., 134: 2678-2684.
PubMedDirect Link - Batra, J. and A. Sood, 2005. Iron deficiency anaemia: Effect on congnitive development in children: A review. Indian J. Clin. Biochem., 20: 119-125.
CrossRefDirect Link - Garcia-Casal, M.N., M. Layrisse, J.P. Pena-Rosas, J. Ramirez, I. Leets and P. Matus, 2003. Iron absorption from elemental iron-fortified corn flakes in humans. Role of vitamins A and C1-3. Nutr. Res., 23: 451-463.
CrossRefDirect Link - Garcia-Casal, M.N., M. Layrisse, L. Solano, M.A. Baron and F. Arguello et al., 1998. Vitamin A and β-carotene can improve nonheme iron absorption from rice, wheat and corn by humans. J. Nutr., 128: 646-650.
Direct Link - Grantham-McGregor, S. and C. Ani, 2001. A review of studies on the effect of iron deficiency on cognitive development in children. J. Nutr., 131: 649S-668S.
Direct Link - Hurrell, R.F., 2002. Fortification: Overcoming technical and practical barriers. J. Nutr., 132: 806S-812S.
PubMedDirect Link - Joshi, M. and R. Gumashta, 2013. Weekly iron folate supplementation in adolescent girls-an effective nutritional measure for the management of iron deficiency Anaemia. Global J. Health Sci., 5: 188-194.
CrossRefDirect Link - Layrisse, M., M.N. Garcia-Casal, L. Solano, M.A. Baron and F. Arguello et al., 1997. The role of vitamin A on the inhibitors of nonheme iron absorption: Preliminary results. J. Nutr. Biochem., 8: 61-67.
CrossRefDirect Link - Mehansho, H., 2006. Iron fortification technology development: New approaches. J. Nutr., 136: 1059-1063.
Direct Link - Roodenburg, A.J.C., C.E. West, R. Hovenier and A.C. Beynen, 1996. Supplemental vitamin a enhances the recovery from iron deficiency in rats with chronic vitamin a deficiency. Br. J. Nutr., 75: 623-636.
CrossRefDirect Link - Rucker, R. and D. Storms, 2002. Interspecies comparisons of micronutrient requirements: Metabolic vs. Absolute body size. J. Nutr., 132: 2999-3000.
Direct Link - Rustanti, N., R. Murwani and S. Anwar, 2011. Acceptability and nutrient content of wet noodles fortified with inorganic or organic iron and provitamin a from pumpkin (Cucurbita moschata). Media Medika Indonesiana, 45: 194-199.
Direct Link - Zimmermann, M.B., R. Biebinger, F. Rohner, A. Dib, C. Zeder, R.F. Hurrell and N. Chaouki, 2006. Vitamin A supplementation in children with poor vitamin A and iron status increases erythropoietin and hemoglobin concentrations without changing total body iron. Am. J. Clin. Nutr., 84: 580-586.
Direct Link - Setyani, S., S.U. Nurdin, E. Arafat and A. Iriyanto, 2004. [Bioavailability and efficiency of ferrous sulfate and citrate in vivo of iron fortified milk candy]. Prosiding Seminar Nasional dan Kongres Perhimpunan Ahli Teknologi Pangan Indonesia (PATPI), December 17-18, 2004, Jakarta, Indonesia, pp: 596-600, (In Indonesian).