G. S. Niture
Department of Animal Biotechnology, College of Veterinary and Animal Sciences, Parbhani 431 402, Maharashtra State, India
A. G. Karpe
KNP College of Veterinary Sciences, Shirwal, India
Minakshi Prasad
Department of Animal Biotechnology, CCS HAU, Hisar, India
A. V. Bhonsle
Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Udgir
S. V. Patil
Department of Animal Biotechnology, College of Veterinary and Animal Sciences, Parbhani 431 402, Maharashtra State, India
ABSTRACT
Rotavirus is a major cause of gastroenteritis in infants and children worldwide and also in wide variety of mammalian and avian species. In the present study, 4 (7.84%) out of the 54 poultry faecal samples tested, were found positive by RNA-PAGE. The samples were of 60-65 weeks age layer poultry birds suffering from diarrhoea. All the PAGE positive samples resolved in to 11 segments with a migration pattern of 5:2:2:2 characteristic of group D avian rotavirus. All the positive samples were of short electropherotype in which segments 10th and 11th migrated very closely. Avian rotavirus samples subjected to VP4 and VP7 gene based RT-PCR did not amplified respective products. RNA-PAGE positive samples were further subjected to VP6 gene based RT-PCR amplification using specific primers. All the four positive samples yielded a specific product of 493 bp. The study reports first ever detection of Group D avian rotavirus in Western India.
PDF References
How to cite this article
G. S. Niture, A. G. Karpe, Minakshi Prasad, A. V. Bhonsle and S. V. Patil, 2010. Detection of Group D Avian Rotaviruses among Layer Poultry from Western India. International Journal of Poultry Science, 9: 72-76.
DOI: 10.3923/ijps.2010.72.76
URL: https://scialert.net/abstract/?doi=ijps.2010.72.76
DOI: 10.3923/ijps.2010.72.76
URL: https://scialert.net/abstract/?doi=ijps.2010.72.76
REFERENCES
- Ahmed, M.S. and M.U. Ahmed, 2006. Detection of avian rotavirus-like virus in broiler chickens in Bangladesh. Bangladesh J. Vet. Med., 4: 73-77.
Direct Link - Chomczynski, P. and N. Sacchi, 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Annu. Rev. Biochem., 162: 156-159.
CrossRefPubMedDirect Link - Estes, M.K. and J. Cohen, 1989. Rotavirus gene structure and function. Microbiol. Rev., 53: 410-449.
Direct Link - Estes, M.K., E.L. Palmer and J.F. Obijeski, 1983. Rotavirus: A review. Curr. Topics Microbiol., 105: 123-184.
PubMedDirect Link - Fedorova, O.F., N.A. Novikova, N.V. Epifanova, L.B. Lukovnikova, O.N. Kniagina, E.V. Gracheva and E.M. Bogacheva, 2005. Optimization of RT-PCR for the identification of VP4 gene of group A rotaviruses and evaluation of its diagnostic efficiency. Vopr Virusol., 50: 39-41.
PubMedDirect Link - Herring, A.J., N.F. Inglis, C.K. Ojeh, D.R. Snodgrass and J.D. Menzies, 1982. Rapid diagnosis of rotavirus infection by direct detection of viral nucleic acid in silver-stained polyacrylamide gels. J. Clin. Microbiol., 16: 473-477.
Direct Link - Ito, H., N. Minamoto, I. Sasaki, H. Goto, M. Sugiyama, T. Kinjo and S. Sugita, 1995. Sequence analysis of cDNA for the VP6 protein of group a avian rotavirus: A comparison with group a mammalian rotaviruses. Arch. Virol., 140: 605-612.
CrossRefDirect Link - Kalica, A.R., R.G. Wyatt and A.Z. Kapikian, 1978. Detection of differences among human and animal rotaviruses using analysis of viral RNA. J. Am. Vet. Med. Assoc., 173: 531-537.
PubMedDirect Link - Kang, G., M. Iturriza-Gomara, J.G. Wheeler, P. Crystal and B. Monica et al., 2004. Quantitation of group A rotavirus by real-time reverse-transcription-polymerase chain reaction: Correlation with clinical severity in children in South India. J. Med. Virol., 73: 118-122.
PubMedDirect Link - Laemmli, U.K., 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227: 680-685.
CrossRefDirect Link - Legrottaglie, R., V. Rizzi and P. Agrimi, 1997. Isolation and identification of avian rotavirus from pheasant chicks with signs of clinical enteritis. Comp. Immunol. Microb., 20: 205-210.
CrossRef - McNulty, M.S., G.M. Allan and J.C. Stuart, 1978. Rotavirus infection in avian species. Vet. Rec., 103: 319-320.
PubMed - Mcnulty, M.S., G. Mallan, D. Todd, J.B. Mcferran and R.M. Mccrcken, 1981. Isolation from chickens of a rotavirus lacking the rotavirus group antigen. J. Gen. Virol., 55: 405-413.
PubMedDirect Link - Mcnulty, M.S., G.M. Alla and J.B. Mcferran, 1984. Prevalence of antibody to conventional and atypical rotaviruses in chickens. Vet. Rec., 114: 219-219.
Direct Link - Mcnulty, M.S., G.M. Allan, D. Todd, B. Mcfarran, E.R. Mckillop, D.S. Colins and R.M. Mccracken, 1980. Isolation of rotaviruses from turkey and chicken, demonstration of distinct serotypes and RNA electropherotypes. Avian Pathol., 9: 363-375.
PubMedDirect Link - Savita, A.L. Kusumakar, Y.P.S. Malik, P. Minakshi and G. Prasad, 2008. Detection and characterization of group A and D avian rotaviruses in India. Indian J. Biotechnol., 7: 554-556.
Direct Link - Svensson, L., I. Uhnoo, M. Grandien and G. Wadell, 1986. Molecular epidemiology of rotavirus infections in Uppsala, Sweden, 1981: Disappearance of a predominant electropherotype. J. Med. Virol., 18: 101-111.
Direct Link - Tamechiro, C.Y., A.F. Alfieri, K.C. Medici and A.A. Alfieri, 2003. Segmented double-stranded genomic RNA viruses in faecal samples from broiler chicken. Braz. J. Microbiol., 34: 349-353.
CrossRefDirect Link - Todd, D. and M.S. McNulty, 1986. Electrophoretic variation of avian rotavirus RNA in polyacrylamide gels. Avian Pathol., 15: 149-159.
PubMedDirect Link - Wani, S.A., M.A. Bhat, S.M. Ishaq, M.A. Ashrafi, A.S. Buchh and M. Haq, 2003. Detection of a mammalian-like group A rotavirus in diarrhoeic chicken. Vet. Microbiol., 94: 13-18.
CrossRef - Yashon, C.V. and K.A. Schat, 1985. Isolation and characterization of avian rotaviruses. Avian Dis., 29: 499-508.
Direct Link
YPS Malik Reply
I have gone through the MS thoroughly and came to know that the virus which authors are declaring group D are not at all group D. Neither they used the primers for group D not the sequences submitted in NCBI are of group D.
I think without analysis, it was submitted and need urgent clarification, before it spreads a wrong message to the scientific community.