Submit Manuscript

Research Journal of Microbiology

Year: 2014 | Volume: 9 | Issue: 1 | Page No.: 1-15
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Article Trend

Total views 302

Authors

Magdy M. Afifi

Houssam M. Atta

Reda A. Bayoumi

Keywords

Research Article

Microbiological Studies on the Production of Antimicrobial Agent by Actinomycete Isolated from Saudi Arabia

Magdy M. Afifi, Houssam M. Atta and Reda A. Bayoumi
This research aims to isolate and identify some actinomycetes, that having antimicrobial substances against some pathogenic bacteria and fungi, from soil samples in Al-Khurmah governorate. Only one actinomycete culture KSA-20 exhibited to produce wide spectrum of antibacterial activities. The nucleotide sequence of the 16S rRNA gene of this strain evidenced an 95% similarity with Streptomyces griseoviridis. From the taxonomic features, the actinomycete isolate KSA-20 matches with Streptomyces griseoviridis in the morphological, physiological and biochemical characters. Thus, it given the suggested name Streptomyces griseoviridis KSA-20. The parameters controlling the biosynthetic process of antibacterial agent were fully investigated. The separation of the active ingredient and its purification were performed using both Thin Layer Chromatography (TLC) and Column Chromatography (CC) techniques. The minimum inhibition concentrations “MICs” of the purified antibiotic were also determined. The purified antibiotic suggestive of being belonging to griseoviridin antibiotic produced by Streptomyces griseoviridis KSA-20.
PDF Fulltext XML References Citation

How to cite this article

Magdy M. Afifi, Houssam M. Atta and Reda A. Bayoumi, 2014. Microbiological Studies on the Production of Antimicrobial Agent by Actinomycete Isolated from Saudi Arabia. Research Journal of Microbiology, 9: 1-15.

URL: https://scialert.net/abstract/?doi=jm.2014.1.15

Related Articles

Biosynthesis of Hygromycin-B Antibiotic by Streptomyces crystallinus AZ151 Isolated from Assuit, Egypt
Identification of Luteomycin like Antibiotic Produced by Streptomyces tanashiensis AZ-C442 Isolated from Luxor Governorate at Upper Egypt
Taificidin1 and Taificidin2, Two Anti-microbial Agents Isolated from the Fermentation Broth of Streptomyces roseodistaticus TA15 and Streptomyces lavendofoliae TA17
Isolation, Characterization, Screening and Antibiotic Sensitivity of Actinomycetes from Locally (Near MCAS) Collected Soil Samples
Isolation and Characterization of a Novel Endophytic Streptomyces SUK 06 with Antimicrobial Activity from Malaysian Plant

Leave a Comment

Your email address will not be published. Required fields are marked *