Isolation and Characterization of a Novel Endophytic Streptomyces SUK 06 with Antimicrobial Activity from Malaysian Plant

Research Article

**Isolation and Characterization of a Novel Endophytic Streptomyces SUK 06 with Antimicrobial Activity from Malaysian Plant**

Norazli Ghadin, Noraziah M. Zin, Vikineswary Sabaratnam, Norhidayah Badya, Dayang F. Basri, Hing H. Lian and Nik M. Sidik

In search of new antimicrobial agents, samples of plant stem which were originally used as traditional medicine were collected from various parts of peninsular Malaysia. The isolate, namely SUK 06 from Thottea grandiflora (family-Aristolchieaceae) was cultured on International Streptomyces Project medium (ISP 2). Macroscopically, the bacteria surface appeared to be smooth with granule texture, irregular folding, dry and chalky and showed a substrate mycelium with grey in color. By scanning electron microscope, this isolate had a unique spiral filament structure. Molecular biological studies on the 16S rRNA revealed that it was distinct from all other genetic accessions of Streptomycetes in GenBank, but bear some genetic similarity to other Streptomycetes. The secondary metabolites extracted by ethyl acetate showed demonstrable killing activity against one or more pathogenic bacteria and inhibition zones (mm) were as followed: Bacilus subtilis (17 mm), Pseudomonas aeruginosa ATCC 27853 (20 mm), Bacillus cereus (22 mm), Pleisiomonas shigelloides (20 mm) and MRSA ATCC 700699 (37 mm). Nevertheless, there were some antifungal activity measured as a percentage of inhibition (%) against Fusarium solani (62%), Aspergillus fumigatus (44%), Pythium ultimum (20%), Phytophthora erythroseptica (23%) and Geothrichum candidum (23%).

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Norazli Ghadin, Noraziah M. Zin, Vikineswary Sabaratnam, Norhidayah Badya, Dayang F. Basri, Hing H. Lian and Nik M. Sidik, 2008. Isolation and Characterization of a Novel Endophytic Streptomyces SUK 06 with Antimicrobial Activity from Malaysian Plant. Asian Journal of Plant Sciences, 7: 189-194.

DOI: 10.3923/ajps.2008.189.194

URL: https://scialert.net/abstract/?doi=ajps.2008.189.194

Asian Journal of Plant Sciences

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Authors

Norazli Ghadin

Noraziah M. Zin

Vikineswary Sabaratnam

Norhidayah Badya

Dayang F. Basri

Hing H. Lian

Nik M. Sidik

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Research Article

**Isolation and Characterization of a Novel Endophytic Streptomyces SUK 06 with Antimicrobial Activity from Malaysian Plant**

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