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Journal of Biological Sciences

Year: 2005 | Volume: 5 | Issue: 5 | Page No.: 643-647
Research Article

Antimicrobial Susceptibility of Campylobacter jejuni and Campylobacter coli Strains Isolated from Humans and Poultry in North of Spain

Gemma De Vega
Facultad de Medicina, Universidad del Pais Vasco (UPV-EHU), Paseo de la Universidad 7, 01006 Vitoria-Gasteiz, Spain
Estibaliz Mateo
Facultad de Medicina, Universidad del Pais Vasco (UPV-EHU), Paseo de la Universidad 7, 01006 Vitoria-Gasteiz, Spain
Agueda Fernandez de Aranguiz
Facultad de Medicina, Universidad del Pais Vasco (UPV-EHU), Paseo de la Universidad 7, 01006 Vitoria-Gasteiz, Spain
Karmele Colom
Facultad de Medicina, Universidad del Pais Vasco (UPV-EHU), Paseo de la Universidad 7, 01006 Vitoria-Gasteiz, Spain
Rodrigo Alonso
Facultad de Medicina, Universidad del Pais Vasco (UPV-EHU), Paseo de la Universidad 7, 01006 Vitoria-Gasteiz, Spain
Aurora Fernandez-Astorga
Departamento de Inmunologia, Microbiologia y Parasitologia, Facultad de Farmacia, Universidad del Pais Vasco (UPV-EHU), Paseo de la Universidad, 7 01006 Vitoria-Gasteiz, Spain
This study was undertaken to evaluate the antimicrobial susceptibility of Campylobacter spp. strains isolated from poultry and human faeces in the Basque Country area, in the North of Spain. Poultry samples at the retail level were randomly purchased in supermarkets and human isolates were obtained from diarrhoea-infected patients. Campylobacter identification was achieved by biochemical tests and species-specific PCR. Susceptibility to seven antibiotics was determined by the agar dilution method following the NCCLS recommendations. Genotyping was carried out by PCR-RFLP of the flaA gene and PFGE profiling. Highest resistance was found for tetracycline (74%) and quinolones: nalidixic acid (76%), ciprofloxacin (72%) and enrofloxacin (61.5%). Decreasing trends in erythromycin resistance was detected in both food and human isolates. Multiresistance to three or more of the drugs was found in 4.8% strains, being more frequent in C. coli (12.5%) than in C. jejuni (2.5%).
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How to cite this article

Gemma De Vega, Estibaliz Mateo, Agueda Fernandez de Aranguiz, Karmele Colom, Rodrigo Alonso and Aurora Fernandez-Astorga, 2005. Antimicrobial Susceptibility of Campylobacter jejuni and Campylobacter coli Strains Isolated from Humans and Poultry in North of Spain. Journal of Biological Sciences, 5: 643-647.

DOI: 10.3923/jbs.2005.643.647

URL: https://scialert.net/abstract/?doi=jbs.2005.643.647

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