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Journal of Biological Sciences
  Year: 2005 | Volume: 5 | Issue: 5 | Page No.: 643-647
DOI: 10.3923/jbs.2005.643.647
 
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Antimicrobial Susceptibility of Campylobacter jejuni and Campylobacter coli Strains Isolated from Humans and Poultry in North of Spain

Gemma De Vega , Estibaliz Mateo , Agueda Fernandez de Aranguiz , Karmele Colom , Rodrigo Alonso and Aurora Fernandez-Astorga

Abstract:
This study was undertaken to evaluate the antimicrobial susceptibility of Campylobacter spp. strains isolated from poultry and human faeces in the Basque Country area, in the North of Spain. Poultry samples at the retail level were randomly purchased in supermarkets and human isolates were obtained from diarrhoea-infected patients. Campylobacter identification was achieved by biochemical tests and species-specific PCR. Susceptibility to seven antibiotics was determined by the agar dilution method following the NCCLS recommendations. Genotyping was carried out by PCR-RFLP of the flaA gene and PFGE profiling. Highest resistance was found for tetracycline (74%) and quinolones: nalidixic acid (76%), ciprofloxacin (72%) and enrofloxacin (61.5%). Decreasing trends in erythromycin resistance was detected in both food and human isolates. Multiresistance to three or more of the drugs was found in 4.8% strains, being more frequent in C. coli (12.5%) than in C. jejuni (2.5%).
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How to cite this article:

Gemma De Vega , Estibaliz Mateo , Agueda Fernandez de Aranguiz , Karmele Colom , Rodrigo Alonso and Aurora Fernandez-Astorga , 2005. Antimicrobial Susceptibility of Campylobacter jejuni and Campylobacter coli Strains Isolated from Humans and Poultry in North of Spain. Journal of Biological Sciences, 5: 643-647.

DOI: 10.3923/jbs.2005.643.647

URL: https://scialert.net/abstract/?doi=jbs.2005.643.647

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