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International Journal of Pharmacology
  Year: 2016 | Volume: 12 | Issue: 8 | Page No.: 801-811
DOI: 10.3923/ijp.2016.801.811
 
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Description of Isolated LAB Producing β-glucan from Egyptian Sources and Evaluation of its Therapeutic Effect

Khalid Abd El Ghany, Ragaa A. Hamouda, Hoda Mahrous , Ekbal Abd Elhafez, Fath Allah H. Ahmed and Hanafy A. Hamza

Abstract:
Background: Lactic Acid Bacteria (LAB) have great therapeutic and nutritional benefits. Among these are: Improved nutritional value of food, control of intestinal infections, improved digestion of lactose, immune modulator effect, control of some types of cancer and control of serum glucose level. Objective: The aim of this study was carried out to evaluate the therapeutic effect of β-glucan extracted from LAB was isolated from different food sources in Egypt. Materials and Methods: The isolation was performed from local Egyptian boza, cider, cheese and yoghurt. Only seven species from 27 was identified as LAB. Only one showed to produce β-glucan and identified by VITEC® 2 as Pediococcus parvulus . The optimization of isolate growth was examined in different temperature, pH and media. Results: The study revealed that the optimum temperature was 37°C, pH was 6 and the most selective medium for growth was Pediococcus selective medium (PSM). Beta-glucan production was assessed by FT-IR analysis, HPLC and HNMR spectroscopy. The therapeutic effect of Pediococcus parvulus β-glucan as antioxidant against DPPH was more significant, cholesterol lowering effect of β-glucan gave valuable results compared to simvastatin drug, the antibacterial effect and control of cancer in vitro against Ehrlich Ascites Carcinoma (EAC) were also gave valuable results. Conclusion: The extracted β-glucan from Egyptian Pediococcus parvulus had different therapeutic effect in vitro including, antioxidative properties, antimicrobial, control of cancer and cholesterol lowering effect.
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How to cite this article:

Khalid Abd El Ghany, Ragaa A. Hamouda, Hoda Mahrous, Ekbal Abd Elhafez, Fath Allah H. Ahmed and Hanafy A. Hamza, 2016. Description of Isolated LAB Producing β-glucan from Egyptian Sources and Evaluation of its Therapeutic Effect. International Journal of Pharmacology, 12: 801-811.

DOI: 10.3923/ijp.2016.801.811

URL: https://scialert.net/abstract/?doi=ijp.2016.801.811

COMMENTS
17 July, 2016
Omar Shaheen:
Good paper
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