International Journal of Pharmacology1811-77751812-5700Asian Network for Scientific Information10.3923/ijp.2016.801.811El GhanyKhalid Abd HamoudaRagaa A. MahrousHoda Abd ElhafezEkbal AhmedFath Allah H. HamzaHanafy A. 82016128Background: Lactic Acid Bacteria (LAB) have great therapeutic and nutritional benefits. Among these are: Improved nutritional value
of food, control of intestinal infections, improved digestion of lactose, immune modulator effect, control of some types of cancer and
control of serum glucose level. Objective: The aim of this study was carried out to evaluate the therapeutic effect of β-glucan extracted
from LAB was isolated from different food sources in Egypt. Materials and Methods: The isolation was performed from local Egyptian
boza, cider, cheese and yoghurt. Only seven species from 27 was identified as LAB. Only one showed to produce β-glucan and identified
by VITEC® 2 as Pediococcus parvulus . The optimization of isolate growth was examined in different temperature, pH and media.
Results: The study revealed that the optimum temperature was 37°C, pH was 6 and the most selective medium for growth was
Pediococcus selective medium (PSM). Beta-glucan production was assessed by FT-IR analysis, HPLC and HNMR spectroscopy. The
therapeutic effect of Pediococcus parvulus β-glucan as antioxidant against DPPH was more significant, cholesterol lowering effect of
β-glucan gave valuable results compared to simvastatin drug, the antibacterial effect and control of cancer in vitro against Ehrlich Ascites
Carcinoma (EAC) were also gave valuable results. Conclusion: The extracted β-glucan from Egyptian Pediococcus parvulus had different
therapeutic effect in vitro including, antioxidative properties, antimicrobial, control of cancer and cholesterol lowering effect.]]>Othman, R.A., M.H. Moghadasian and P.J. Jones,201169299309Cloetens, L., M. Ulmius, A. Johansson-Persson, B. Akesson and G. Onning,201270444458Lam, K.L. and P.C.K. Cheung,201324564Tabesh, F., H. Sanei, M. Jahangiri, A. Momenizadeh, E. Tabesh, K. Pourmohammadi and M. Sadeghi,20142014Feller, S.,20152015McIntosh, M., B.A. Stone and V.A. Stanisich,200568163173Garai-Ibabe, G., J. Areizaga, R. Aznar, P. Elizaquivel, A. Prieto, A. Irastorza and M.T. Duenas,20105861496156Anderson, J.W., L. Story, B. Sieling, W.J. Chen, M.S. Petro and J. Story,19844011461155Battilana, P., K. Ornstein, K. Minehira, J.M. Schwarz and K. Acheson et al.,200155327333Bourdon, I., W. Yokoama, P. Davis, C. Hudson and R. Backus et al.,1999695563Juntunen, K.S., L.K. Niskanen, K.H. Liukkonen, K.S. Poutanen, J.J. Holst and H.M. Mykkanen,200275254262Wood, P.J.,2007β-glucans in diet and health.]]>46230238Whelton, S.P., A.D. Hyre, B. Pedersen, Y. Yi, P.K. Whelton and J. He,200523475481Ferrannini, E., G. Buzzigoli, R. Bonadonna, M.A. Giorico and M. Oleggini et al.,1987317350357De Man, J.C., M. Rogosa and M.E. Sharpe,1960Lactobacilli.]]>23130135Nakanishi, I., K. Kimura, T. Suzuki, M. Ishikawa, I. Banno, T. Sakane and T. Harada,197622111Holt, J.G., N.R. Krieg, P.H.A. Sneath, J.T. Staley and S.T. Williams,19949th Edn.,Pages: 787Pages: 787Lapage, S.P., J.E. Shelton, T.G. Mitchell and A.R. Mackenzie,19701970pp: 135-228pp: 135-228Simpson, P.J., G.F. Fitzgerald, C. Stanton and R.P. Ross,200664120125Jackson, D.C., C.J. Mueller, I. Dolski, K.M. Dalton and J.B. Nitschke et al.,200314612617Lee, Y.,20082008Demirci, M. and H. Gunduz,1994Velasco, S., E. Arskold, M. Paese, A. Grage, A. Irastorza, P. Radstrom and E.W.J. van Niel,2006Pediococcus parvulus 2.6.]]>111252258Synytsya, A. and M. Novak,201421717Alaubydi, M.A. and R.M. Abed,20113535541Silverstein, R.M., F.X. Webster, D.J. Kiemle and D.L. Bryce,20145th Edn.,Pages: 464Pages: 464El Ghany, K.A., E.A. Elhafez, R.A. Hamouda, H. Mahrous, F.A.H. Ahmed and H.A. Hamza,2014Lactobacillus acidophilus bacteria isolated from Egyptian infants.]]>10282288Kamal, S., R.A. Hamouda, H. Mahrous, M.L. Salem, H.A. Hamza and E. Abd Elhafez,2015In vitro treatment with intact cells or cell lysates of Lactobacillus and Spirulina induced lowering effects on induced hypercholesteremia.]]>11638643Freshney, R.I.,20055th Edn.,pp: 387-389pp: 387-389Pérez-Rodríguez, C., W.M. Pauli and P. Bazevque,199015113115Snedecor, G.W. and W.G. Cochran,19807th Edn.,Pages: 507Pages: 507Hoda, M., K. El-Halafawy and M.A.E. El-Soda,2010Pages: 192Pages: 192Nel, H.A., R. Bauer, E.J. Vandamme and L.M.T. Dicks,2001Pediococcus damnosus NCFB 1832 and the influence of pH and nutrients on the production of pediocin PD-1.]]>9111311138Gomes, A.M.P. and F.X. Malcata,1999 Bifidobacterium spp. and Lactobacillus acidophilus: Biological, biochemical, technological and therapeutical properties relevant for use as probiotics.]]>10139157Klaenhammer, T.R.,2000130415S416SLin, M.Y. and F.J. Chang,2000Bifidobacterium longum ATCC 15708 and Lactobacillus acidophilus ATCC 4356.]]>4516171622El Khoury, D., C. Cuda, B.L. Luhovyy and G.H. Anderson,20122012Doleyres, Y. and C. Lacroix,200515973988Chinnu, K., M. Muthukumaran, S. Mukund and V. Sivasubramanian,2014Chroococcus turgidus.]]>4217220