Phytochemical Screening, Total Phenolics and Antioxidant and Antibacterial Activities of Callus from Brassica nigra L. Hypocotyl Explants

Research Article

**Phytochemical Screening, Total Phenolics and Antioxidant and Antibacterial Activities of Callus from Brassica nigra L. Hypocotyl Explants**

E.A. Hussein, A.M. Taj-Eldeen, A.S. Al-Zubairi, A.S. Elhakimi and A.R. Al-Dubaie

In the present study, callus was successfully obtained from hypocotyl explants of Brassica nigra (L.) in vitro under both dark and light incubation conditions separately using Murashige and Skoog’s basal salts enriched with sucrose, benzyladenine (BA), Naphthalene Acetic Acid (NAA) and 2,4-Dichlorophenoxyacetic acid (2,4 D). Callus growth curve was plotted on the basis of single callus dry weight in grams versus time in weeks. The obtained calli were subjected to phytochemical screening for some secondary plant metabolites using thin layer chromatography, determination of total phenolics using Folin Ciocalteu reagent, estimation of the antioxidant activity by the scavenging activity of DPPH free radicals and screening calli extracts for antibacterial activity against 4 pathogenic bacterial strains. Except for phytochemical screening which was carried out at the end of the growth period only, other analyses were carried out when calli were 2, 4 and 6 weeks old. Results of the present study have revealed that calli were rich in secondary metabolites as they gave positive results for testes of volatile oils, anthraquinones, flavonoids and tannins. Although there were some exceptions, it was generally observed that total phenolic, antioxidant activity and antibacterial activity were higher in calli obtained under light incubation conditions than calli obtained under dark incubation conditions or the mother plant parts from which calli were induced. It is also observed that older calli accumulated more amounts of total phenolics, exhibited higher antioxidant activity and stronger antibacterial activity.

PDF Fulltext XML References Citation

How to cite this article

E.A. Hussein, A.M. Taj-Eldeen, A.S. Al-Zubairi, A.S. Elhakimi and A.R. Al-Dubaie, 2010. Phytochemical Screening, Total Phenolics and Antioxidant and Antibacterial Activities of Callus from Brassica nigra L. Hypocotyl Explants. International Journal of Pharmacology, 6: 464-471.

DOI: 10.3923/ijp.2010.464.471

URL: https://scialert.net/abstract/?doi=ijp.2010.464.471

International Journal of Pharmacology

Article Trend

Total views 508

Search

Authors

E.A. Hussein

A.M. Taj-Eldeen

A.S. Al-Zubairi

A.S. Elhakimi

A.R. Al-Dubaie

Keywords

Research Article

**Phytochemical Screening, Total Phenolics and Antioxidant and Antibacterial Activities of Callus from Brassica nigra L. Hypocotyl Explants**

How to cite this article

Related Articles

Leave a Comment