Subscribe Now Subscribe Today
Science Alert
 
FOLLOW US:     Facebook     Twitter
Blue
   
Curve Top
International Journal of Pharmacology
  Year: 2010 | Volume: 6 | Issue: 4 | Page No.: 464-471
DOI: 10.3923/ijp.2010.464.471
Phytochemical Screening, Total Phenolics and Antioxidant and Antibacterial Activities of Callus from Brassica nigra L. Hypocotyl Explants
E.A. Hussein, A.M. Taj-Eldeen, A.S. Al-Zubairi, A.S. Elhakimi and A.R. Al-Dubaie

Abstract:
In the present study, callus was successfully obtained from hypocotyl explants of Brassica nigra (L.) in vitro under both dark and light incubation conditions separately using Murashige and Skoog’s basal salts enriched with sucrose, benzyladenine (BA), Naphthalene Acetic Acid (NAA) and 2,4-Dichlorophenoxyacetic acid (2,4 D). Callus growth curve was plotted on the basis of single callus dry weight in grams versus time in weeks. The obtained calli were subjected to phytochemical screening for some secondary plant metabolites using thin layer chromatography, determination of total phenolics using Folin Ciocalteu reagent, estimation of the antioxidant activity by the scavenging activity of DPPH free radicals and screening calli extracts for antibacterial activity against 4 pathogenic bacterial strains. Except for phytochemical screening which was carried out at the end of the growth period only, other analyses were carried out when calli were 2, 4 and 6 weeks old. Results of the present study have revealed that calli were rich in secondary metabolites as they gave positive results for testes of volatile oils, anthraquinones, flavonoids and tannins. Although there were some exceptions, it was generally observed that total phenolic, antioxidant activity and antibacterial activity were higher in calli obtained under light incubation conditions than calli obtained under dark incubation conditions or the mother plant parts from which calli were induced. It is also observed that older calli accumulated more amounts of total phenolics, exhibited higher antioxidant activity and stronger antibacterial activity.
 [Fulltext PDF]   [Fulltext HTML]   [XML: Abstract + References]   [References]   [View Citation]  [Report Citation]
 RELATED ARTICLES:
  •    Phytochemical Assessment and Antioxidant Activities of Alcoholic and Aqueous Extracts of Acacia mearnsii De Wild
  •    Plant as a Source of Natural Antiviral Agents
  •    How to Cope with Dengue in Developing Countries Like Pakistan?
  •    A Review on the Recent Advances in Pharmacological Studies on Medicinal Plants; Animal Studies are Done but Clinical Studies Needs Completing
  •    Screening for Antibacterial Properties of Some Iranian Plants Against Two Strains of Escherichia coli
  •    Antioxidative and Antibacterial Activities of Pangium edule Seed Extracts
  •    Variation of Callus Induction and Active Metabolite Accumulation in Callus Cultures of Two Varieties of Ricinus communis L.
How to cite this article:

E.A. Hussein, A.M. Taj-Eldeen, A.S. Al-Zubairi, A.S. Elhakimi and A.R. Al-Dubaie, 2010. Phytochemical Screening, Total Phenolics and Antioxidant and Antibacterial Activities of Callus from Brassica nigra L. Hypocotyl Explants. International Journal of Pharmacology, 6: 464-471.

DOI: 10.3923/ijp.2010.464.471

URL: https://scialert.net/abstract/?doi=ijp.2010.464.471

 
COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 
 
 
 

Curve Bottom