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Pakistan Journal of Nutrition
  Year: 2007 | Volume: 6 | Issue: 1 | Page No.: 94-98
DOI: 10.3923/pjn.2007.94.98
Detection of Cow Milk in Water Buffalo Cheese by SYBR Green Real-Time PCR: Sensitivity Test on Governing Liquid Samples
Maria Feligini , Naima Alim , Ivan Bonizzi , Giuseppe Enne and Riccardo Aleandri

Abstract:
A real-time polymerase chain reaction (PCR) assay was developed to detect a bovine-specific mitochondrial DNA sequence in `buffalo` Mozzarella cheese by using primers targeting the cytochrome oxidase subunit 1 (COI) gene. Hot-start PCR, primer design, annealing and signal acquisition temperatures were exploited to obtain reliable analytical conditions which yielded a 134-bp amplicon from cow`s DNA only. Water buffalo`s DNA didn`t originate any amplification product. DNA isolated from blood was used to test primers` specificity and to construct a calibration curve in order to quantify bovine DNA concentration in governing liquid. The method was capable to detect low amounts of cow`s DNA in governing liquid samples. Due to low detection limit and fast, simple execution, the analytical protocol described in this work is suitable to become a common tool to detect the fraudulent addition of cow milk in water buffalo Mozzarella cheese.
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How to cite this article:

Maria Feligini , Naima Alim , Ivan Bonizzi , Giuseppe Enne and Riccardo Aleandri , 2007. Detection of Cow Milk in Water Buffalo Cheese by SYBR Green Real-Time PCR: Sensitivity Test on Governing Liquid Samples . Pakistan Journal of Nutrition, 6: 94-98.

DOI: 10.3923/pjn.2007.94.98

URL: https://scialert.net/abstract/?doi=pjn.2007.94.98

 
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