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Journal of Biological Sciences
  Year: 2012 | Volume: 12 | Issue: 5 | Page No.: 294-300
DOI: 10.3923/jbs.2012.294.300
 
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Different Lipolytic Response to (-)-Epigallocatechin-3-gallate in Adipocytes Derived from Normal Diet-fed Rats and High Fat Diet-fed Rats
Pawitra Pulbutr, Atika Jaruchotikamol, Jirawan Chaiyachart, Pannee Sonrasee and Wannaporn Poungpan

Abstract:
(-)-Epigallocatechin-3-gallate (EGCG), the major catechins found in green tea, has been known to possess various pharmacological activities. It has been demonstrated that EGCG affects on several adipocyte functions, involving in its weight-controlling action. This study was aimed to investigate the effects of EGCG on adipocyte lipolysis in adipocytes derived from Normal Pellet Diet (NPD)-fed rats and High Fat Diet (HFD)-fed rats. Sixteen male Wistar rats were divided into 2 groups (8 rats per group). The rats in each group were fed with NPD or HFD for 3 weeks. The adipocyte suspensions were prepared by the collagenase digestion method. The concentration of Free Fatty Acid (FFA) was measured after 1 h-incubation as an index of adipocyte lipolysis. EGCG at every concentration tested (1, 5 and 10 μM) significantly stimulated basal lipolysis in adipocytes derived from the NPD-fed rats with the FFA concentrations of 453.67±25.57, 458.87±26.84 and 443.97±19.75 μM mL-1 Packed Cell Volume (PCV)/h, respectively (p<0.05). In adipocytes derived from the HFD-fed rat, EGCG only at the concentration of 5 μM significantly increased basal adipocyte lipolysis with the FFA concentration of 1,091.75±220.56 μM mL-1 PCV h-1 (p<0.05; n = 4). Conversely, EGCG at any of the concentrations tested did not cause a significant change in isoprenaline (0.1 μM)-induced lipolysis in both adipocyte groups. These results indicate a lower lipolytic action of EGCG in adipocytes derived from the HFD-fed rats. Further experiments thus should be done to explore the reason for the difference in the lipolytic response to EGCG between these adipocytes.
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How to cite this article:

Pawitra Pulbutr, Atika Jaruchotikamol, Jirawan Chaiyachart, Pannee Sonrasee and Wannaporn Poungpan, 2012. Different Lipolytic Response to (-)-Epigallocatechin-3-gallate in Adipocytes Derived from Normal Diet-fed Rats and High Fat Diet-fed Rats. Journal of Biological Sciences, 12: 294-300.

DOI: 10.3923/jbs.2012.294.300

URL: https://scialert.net/abstract/?doi=jbs.2012.294.300

 
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