Journal of Biological Sciences1727-30481812-5719Asian Network for Scientific Information10.3923/jbs.2012.294.300PulbutrPawitra JaruchotikamolAtika ChaiyachartJirawan SonraseePannee PoungpanWannaporn 52012125(-)-Epigallocatechin-3-gallate (EGCG), the major catechins
found in green tea, has been known to possess various pharmacological activities.
It has been demonstrated that EGCG affects on several adipocyte functions, involving
in its weight-controlling action. This study was aimed to investigate the effects
of EGCG on adipocyte lipolysis in adipocytes derived from Normal Pellet Diet
(NPD)-fed rats and High Fat Diet (HFD)-fed rats. Sixteen male Wistar rats were
divided into 2 groups (8 rats per group). The rats in each group were fed with
NPD or HFD for 3 weeks. The adipocyte suspensions were prepared by the collagenase
digestion method. The concentration of Free Fatty Acid (FFA) was measured after
1 h-incubation as an index of adipocyte lipolysis. EGCG at every concentration
tested (1, 5 and 10 μM) significantly stimulated basal lipolysis in adipocytes
derived from the NPD-fed rats with the FFA concentrations of 453.67±25.57,
458.87±26.84 and 443.97±19.75 μM mL-1 Packed Cell
Volume (PCV)/h, respectively (p<0.05). In adipocytes derived from the HFD-fed
rat, EGCG only at the concentration of 5 μM significantly increased basal
adipocyte lipolysis with the FFA concentration of 1,091.75±220.56 μM
mL-1 PCV h-1 (p<0.05; n = 4). Conversely, EGCG at any
of the concentrations tested did not cause a significant change in isoprenaline
(0.1 μM)-induced lipolysis in both adipocyte groups. These results indicate
a lower lipolytic action of EGCG in adipocytes derived from the HFD-fed rats.
Further experiments thus should be done to explore the reason for the difference
in the lipolytic response to EGCG between these adipocytes.]]>Arner, P.,200519471482Auvichayapat, P., M. Prapochanung, O. Tunkamnerdthai, B. Sripanidkulchai and N. Auvichayapat et al.,200893486491Balentine, D.A., S.A. Wiseman and L.C. Bouwens,199737693704Biro, F.M. and M. Wien,2010911499S1505SBost, F., M. Aouadi, L. Caron, P. Even and N. Belmonte et al.,2005in vitro and in vivo adipogenesis.]]>54402411Cash, S.W., S.A. Beresford, J.A. Henderson, A. McTiernan, L. Xiao, C.Y. Wang and D.L. Patrick,2011619Chan, C.Y., L. Wei, F. Castro-Munozledo and W.L. Koo,201189779785Chen, Y.K., C. Cheung, K.R. Reuhl, A.B. Liu, M.J. Lee, Y.P. Lu and C.S. Yang,2011591186211871Collin, P., N. Chapados, E. Dufresne, P. Corriveau, P. Imbeault and J.M. Lavoie,2006in vitro lipolysis of intra-abdominal fat depots in relation to high-fat diet-induced hepatic steatosis in rats.]]>96268275Gaidhu, M.P., N.M. Anthony, P. Patel, T.J. Hawke and R.B. Ceddia,2010298C961C971Gehart, H., S. Kumpf, A. Ittner and R. Ricci,201011834840Greenberg, A.S., W.J. Shen, K. Muliro, S. Patel, S.C. Souza, R.A. Roth and F.B. Kraemer,20012764545645461Harris, R.B.,199112110751080Ito, A., T. Suganami, Y. Miyamoto, Y. Yoshimasa, M. Takeya, Y. Kamei and Y. Ogawa,20072822544525452Kopelman, P.G.,2000404635643Lee, M.S., C.T. Kim and Y. Kim,200954151157Lee, M.S., C.T. Kim, I.H. Kim and Y. Kim,20092310881091Luppino, F.S., L.M. de Wit, P.F. Bouvy, T. Stijnen, P. Cuijpers, B.W. Penninx and F.G. Zitman,201067220229Naim, M., J.G. Brand, M.R. Kare and R.G. Carpenter,198511514471458Ogasawara, J., K. Kitadate, H. Nishioka, H. Fujii and T. Sakurai et al.,201125467471Pulbutr, P., K. Thunchomnang, K. Lawa, A. Mangkhalathon and P. Saenubol,20117629634Rodbell, M.,1964239375380Srinivasan, K., B. Viswanad, L. Asrat, C.L. Kaul and P. Ramarao,200552313320Sung, H.Y., C.G. Hong, Y.S. Suh, H.C. Cho and J.H. Park et al.,2010382303310Thielecke, F. and M. Boschmann,2009701124Von Lengerke, T. and C. Krauth,201169220229