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International Journal of Pharmacology

Year: 2018 | Volume: 14 | Issue: 4 | Page No.: 546-552
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Authors

Amjed Haseeb Khamees

Ali Jabbar Abdulhussein

Hayder Bahaa Sahib

Hayder Bahaa Sahib

Hayder Adnan Fawzi

Keywords

Background and Objective: Cyperus rotundus (C. rotundus) has been used in medicine years ago. The aim of the study was to investigate the possible anti-angiogenic and antioxidant activity and to find the different phytochemicals present in Cyperus rotundus ethanol extract that may has anti-angiogenic activity. Methodology: Qualitative analysis of various secondary metabolites by specific chemical tests was carried out on the ethanol extract. The ex vivo rat aorta ring assay was used to screen the extract for possible anti-angiogenic activity, this assay was also used to determine the dose-response effect of the active extract. Six concentrations of crude extract were tested (100, 50, 25, 12.5, 6.25 and 3.125 μg mL–1) on rat aortic rings to determine the dose response curve. Free radical scavenging activity of extract was determined by 1, 1-Diphenyl-2-picrylhydrazyl (DPPH) assay. Results: Cyperus rotundus ethanol extract showed significant dose-dependent blood vessels inhibition in comparison to the negative control (p<0.05). Ethanol extract showed significant free radical scavenging activity. Phytochemical investigation of alcoholic extract indicated the presence of various chemical constituents like alkaloids, glycosides, steroids, tannins, carbohydrates and flavonoids. Conclusion: The anti- angiogenic activity showed by the ethanol extract may be due to the presence of antioxidant compounds.
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