International Journal of Pharmacology1811-77751812-5700Asian Network for Scientific Information10.3923/ijp.2018.546.552Cyperus rotundus Ethanol Extract]]>Haseeb KhameesAmjed Jabbar AbdulhusseinAli Bahaa SahibHayder Adnan FawziHayder 42018144Background and Objective:Cyperus rotundus (C. rotundus) has been used in medicine years ago. The aim of the study was to investigate the possible anti-angiogenic and antioxidant activity and to find the different phytochemicals present in Cyperus rotundus ethanol extract that may has anti-angiogenic activity. Methodology: Qualitative analysis of various secondary metabolites by specific chemical tests was carried out on the ethanol extract. The ex vivo rat aorta ring assay was used to screen the extract for possible anti-angiogenic activity, this assay was also used to determine the dose-response effect of the active extract. Six concentrations of crude extract were tested (100, 50, 25, 12.5, 6.25 and 3.125 μg mL1) on rat aortic rings to determine the dose response curve. Free radical scavenging activity of extract was determined by 1, 1-Diphenyl-2-picrylhydrazyl (DPPH) assay. Results:Cyperus rotundus ethanol extract showed significant dose-dependent blood vessels inhibition in comparison to the negative control (p<0.05). Ethanol extract showed significant free radical scavenging activity. Phytochemical investigation of alcoholic extract indicated the presence of various chemical constituents like alkaloids, glycosides, steroids, tannins, carbohydrates and flavonoids. Conclusion: The anti- angiogenic activity showed by the ethanol extract may be due to the presence of antioxidant compounds.]]>Cirujeda, A., A. Anzalone, J. Aibar, M.M. Moreno and C. Zaragoza,2012Cyperus rotundus L.) control with paper mulch in processing tomato.]]>396671Ates, D.A. and O.T. Erdogrul,200327157162Duarte, M.C.T., G.M. Figueira, A. Sartoratto, V.L.G. Rehder and C. Delarmelina,2005Candida activity of Brazilian medicinal plants.]]>97305311Sundaram, M.S., T. Sivakumar and G. Balamurugan,2008Cyperus rotundus Linn. Leaves on acute and subacute inflammation in experimental rat models.]]>28302304Raut, N.A. and N.J. Gaikwad,2006Cyperus rotundus in alloxan induced diabetes in rats.]]>77585588Kilani, S., R.B. Ammar, I. Bouhlel, A. Abdelwahed and N. Hayder et al.,2005Cyperus rotundus as antimutagens and radical scavengers.]]>20478484Zhu, M., H.H. Luk, H.S. Fung and C.T. Luk, 1997Cyperus rotundus against ethanol induced gastric ulceration in rats.]]>11392394Kilani, S., I. Bouhlel, R.B. Ammar, M.B. Shair and I. Skandrani et al.,2007Cyperus rotundus. Correlation with their antiradical and antimutagenic properties.]]>2007Dhillon, R.S., S. Singh, S. Kundra and A.S. Basra,1993Cyperus rotundus Linn.]]>138993Pal, D.K. and S. Dutta,2006Cyperus rotundus L.]]>68256258Al-Zubaidy, A.A., H.B. Sahib and M.H. Sadiq,2016Fenugreek extracts: In vivo and ex vivo study.]]>36184189Jasim, G.A., A.A. Alzubaidy, S.M. Hussain and H.B. Sahib,2015Commiphora molmol oleo-gum-resin extracts.]]>338390Sahib, H.B., A.F. Aisha, M.F. Yam, M.Z. Asmawi and Z. Ismail et al.,2009Orthosiphon stamineus benth. Methanolic leaves extract.]]>5162167Swift, M.E., H.K. Kleinman and L.A. DiPietro,19997914791487Sarker, S.D. and L. Nahar,20122012pp: 1-25pp: 1-25Brown, K.J., S.F. Maynes, A. Bezos, D.J. Maguire, M.D. Ford and C.R. Parish,1996in vitro assay for human angiogenesis.]]>75539555Nicosia, R.F., Y.L. Lin, D. Hazelton and X. Qian,199715113791386Sharma, O.P. and T.K. Bhat,200911312021205Stiffey-Wilusz, J., J.A. Boice, J. Ronan, A.M. Fletcher and M.S. Anderson,2001ex vivo angiogenesis assay utilizing commercial porcine carotidartery: Modification of the rat aortic ring assay.]]>439Sahib, H.B., N.A.H. Harchan, S.A.M. Atraqchi and A.A. Abbas,20106616623Soumaya, K.J., G. Zied, N. Nouha, K. Mounira, G. Kamel, F.D.M. Genvieve and G.C. Leila,2014in vitro antioxidant and apoptotic activities of Cyperus rotundus.]]>7105112Carmeliet, P.,20039653660Sahib, H.B., A.A. Al-Zubaidy, S.M. Hussain and G.A. Jassim,2014Vitex agnus castus leaves extracts.]]>6863869Prauchner, C.A.,20142719