
	International Journal of Cancer Research
	Year: 2010 \| Volume: 6 \| Issue: 1 \| Page No.: 19-26 DOI: 10.3923/ijcr.2010.19.26

Detection of Novel Genomic Polymorphism in Acute Lymphoblastic Leukemia by Random Amplified Polymorphic DNA Analysis

Mohammed A. Ibrahim, Noha Saleh, Esmael Archoukieh, Haithum W. Al-Obaide, Mohannad M. Al-Obaidi and Harun M. Said

Abstract: Molecular genetic studies suggested that genetic instabilities of genomic DNA are implicated in the pathogenesis of cancers. In this study the molecular genomic polymorphism of acute lymphoblastic leukemia was analyzed by 23 arbitrary primers of decamer oligonucleotides to investigate the genetic correlation by cluster tree analysis. Ten primers were able to amplify genomic DNA of leukemia patients, but varied in their ability to detect genomic DNA instability. One of amplifying primers showed novel pattern of amplified DNA fragments. It amplified DNA fragment (3.162 kbp) in normal individuals but was absent in 77.8% of patients, whereas other amplified DNA fragment (0.98 kbp) was absent in normal individuals but it was present in 55.6% of patients. Moreover, dissimilarity matrix analysis of amplified DNA fragments provided genetic relatedness among genomic DNA of patients.

PDF Fulltext XML References Citation Report Citation

RELATED ARTICLES:

•	Molecular Diagnostics and Therapy of Acute Myeloid Leukemia

•	Epigenetics and Esophageal Cancer: Role of Altered Methylation in Specific Genes

•	Perspective of DNA Methylation in Cancer Research

•	Prospects for DNA Methylation Research in Psychiatric Disorders

•	Molecular Characterization of Pomegranate (Punica granatum L.) Landraces Grown in Jordan using Amplified Fragment Length Polymorphism Markers

How to cite this article:

Mohammed A. Ibrahim, Noha Saleh, Esmael Archoukieh, Haithum W. Al-Obaide, Mohannad M. Al-Obaidi and Harun M. Said, 2010. Detection of Novel Genomic Polymorphism in Acute Lymphoblastic Leukemia by Random Amplified Polymorphic DNA Analysis. International Journal of Cancer Research, 6: 19-26.

DOI: 10.3923/ijcr.2010.19.26

URL: https://scialert.net/abstract/?doi=ijcr.2010.19.26

COMMENT ON THIS PAPER

Navigation


		Online First
		Current Issue
		Previous Issues
		Editorial Board
		Submit a Manuscript
		Guide to Authors
		Subscribe to E-alerts

Indexed In


		ASCI-Database
		Asian Digital Library
		Cambridge Scientific Abstract
		Chemical Abstract Services
		FSTA
		SCIMAGO

Science Alert