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Biotechnology

Year: 2010 | Volume: 9 | Issue: 4 | Page No.: 499-505
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Authors

N. Al-Hmoud

H. Al-Rousan

B.O. Hayek

M.A. Ibrahim

Keywords

Research Article

Detection of Genetically Modified Maize and Soybean Food Products in the Jordanian Market

N. Al-Hmoud, H. Al-Rousan, B.O. Hayek and M.A. Ibrahim
The objective of this study was to survey for the genetically modified maize and soybean food products in the Jordanian market. The study was designed to extract genomic DNA of maize and soybean products by cetyltrimethylammonium bromide (CTAB) method and to identify specific genes for maize and soybean, expression control specific genes 35S promoter and NOS terminator by polymerase chain reaction analysis. For confirmation test of the genetically modified maize and soybean food products, nested polymerase chain reaction experiments were performed using internal primers for the detection of the E35S promoter and the hsp70 exon1/intron1 region of maize MON810 and Cp4 EPSPS gene of soybean. Three out of 19 maize food products were identified as carrying amplified DNA fragments of 35S promoter region, the nested PCR test confirmed the presence of MON810 event. One of three soybean food products was identified as carrier DNA fragments of 35S promoter region, Cp4 EPSPS event was not detected.
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