Submit Manuscript

Asian Journal of Plant Sciences

Year: 2023 | Volume: 22 | Issue: 4 | Page No.: 628-636
Research Article

Effect of Ethyl Methane Sulfonate on the Mutants Inducement in Balsam Plants (Impatiens balsamina Linn.)

Nguyen Thi Pha
Institute of Food and Biotechnology, Can Tho University, Xuan Khanh, Ninh Kieu, Can Tho, Vietnam
Bui Minh Sang
Institute of Food and Biotechnology, Can Tho University, Xuan Khanh, Ninh Kieu, Can Tho, Vietnam
Mai Thanh Thao
Institute of Food and Biotechnology, Can Tho University, Xuan Khanh, Ninh Kieu, Can Tho, Vietnam
Tran Dinh Gioi
Cuu Long Delta Rice Research Institute, Thoi Thanh, Thoi Lai, Can Tho, Vietnam
LiveDNA: 84.41891
ORCID: 0000-0001-6798-3185
Background and Objective: Balsam (Impatiens balsamina Linn.) is a popular ornamental plant with beautiful colored flowers and decorated plant shapes. This research has been conducted on the application of chemical mutagens ethyl methane sulfonate (EMS) in breeding balsam flowers to create diverse variations and enhance the decorative and aesthetic value of this plant species. Materials and Methods: Seeds of purple balsam flower were treated with EMS at different concentrations (0, 0.4, 0.6 and 0.8% v/v) for 3 days before incubation and sowing in pots. The studied parameters include germination rate, mortality rate compared to the control (%), survival ability through different stages (%), frequency and mutation efficiency, as well as the morphological characteristics of balsam flowers. Results: All doses of EMS treatments reduced the germination rate and plant survival rate. The EMS treatment changed the plant height and leaf length but had no significant effect on the stem diameter, leaf width and leaf area of balsam plants. A total of 10 morphological mutations were detected. In which, mutant related to floral color such as E3 (dark purple), E5 (pinkish-orange) and E9 (pale purple with partial loss of pigmentation in the lower petal margin), especially E7 (light pink) and E8 (reddish-purple) with more flowers than that of the control, indicating their potential to develop into new varieties. Conclusion: Ten morphological mutations were detected and several of them had various noteworthy characteristics that potential to develop into new varieties. The treatment concentration of 0.6% EMS for 3 days was the most suitable option with the highest mutation frequency and efficiency of 14.28 and 0.71%, respectively.
PDF Fulltext XML References Citation

Leave a Comment

Your email address will not be published. Required fields are marked *

Article Trend

Total views 5182

References

  1. Panda, P., A. Nigam and G.P. Rao, 2021. Multilocus gene analysis reveals the presence of two phytoplasma groups in Impatiens balsamina showing flat stem and phyllody. 3 Biotech, Vol. 11.
    CrossRefDirect Link
  2. Lu, Z., J. Cui, L. Wang, N. Teng and S. Zhang et al., 2021. Genome-wide DNA mutations in arabidopsis plants after multigenerational exposure to high temperatures. Genome Biol., Vol. 22.
    CrossRefDirect Link
  3. Singer, S.D., J.D. Laurie, A. Bilichak, S. Kumar and J. Singh, 2021. Genetic variation and unintended risk in the context of old and new breeding techniques. Crit. Rev. Plant Sci., 40: 68-108.
    CrossRefDirect Link
  4. Chaudhary, J., A. Alisha, V. Bhatt, S. Chandanshive and N. Kumar et al., 2019. Mutation breeding in tomato: Advances, applicability and challenges. Plants, Vol. 8.
    CrossRefDirect Link
  5. Amini, M., 2014. Ethyl Methanesulfonate. In: Encyclopedia of Toxicology, Wexler, P. (Ed.), Academic Press, Cambridge, Massachusetts, ISBN: 9780123864550, pp: 522-524.
    CrossRefDirect Link
  6. Sarmiento, F.B., J.A. Leigh and W.B. Whitman, 2011. Genetic Systems for Hydrogenotrophic Methanogens. In: Methods in Enzymology, Rosenzweig, A.C. and S.W. Ragsdale (Eds.), Academic Press, Cambridge, Massachusetts, ISBN: 978-0-12-385112-3, pp: 43-73.
    CrossRefDirect Link
  7. Altindal, D. and N. Altindal, 2018. Effect of ethyl methanesulfonate (EMS) applications on the growth of sunflower (Helianthus annuus L. cv. Palancı-I) in vitro saline conditions. J. Iğdır Univ. Inst. Sci. Technol., 8: 351-359.
    CrossRefDirect Link
  8. Roychowdhury, R. and J. Tah, 2011. Assessment of chemical mutagenic effects in mutation breeding programme for M1 generation of carnation (Dianthus caryophyllus). Res. Plant Biol., 1: 23-32.
    Direct Link
  9. Rao, G.M. and V.M. Rao, 1983. Mutagenic efficiency, effectiveness and factor of effectiveness of physical and chemical mutagens in rice. Cytologia, 48: 427-436.
    CrossRefDirect Link
  10. Ananthaswamy, H.N., U.K. Vakil and A. Sreenivasan, 1971. Biochemical and physiological changes in gamma-irradiated wheat during germination. Radiat. Bot., 11: 1-12.
    CrossRefDirect Link
  11. Joshi, N., A. Ravindran and V. Mahajan, 2011. Investigations on chemical mutagen sensitivity in onion (Allium cepa L.). Int. J. Bot., 7: 243-248.
    CrossRefDirect Link
  12. Raina, A. and Samiullah Khan, 2020. Mutagenic effectiveness and efficiency of gamma rays and sodium azide in M2 generation of cowpea [Vigna unguiculata (L.) Walp.]. bioRxiv.
    CrossRefDirect Link
  13. Yongfu, Z., L. Guizhi, X. Yingmei, H. Heping, Y. Lixin and L. Jiani, 2015. Study on mutagenic effects of ethyl methane sulfonate on impatiens balsamina. North. Hortic., 21: 83-89.
    Direct Link

Keywords