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International Journal of Botany
  Year: 2011 | Volume: 7 | Issue: 3 | Page No.: 243-248
DOI: 10.3923/ijb.2011.243.248
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Investigations on Chemical Mutagen Sensitivity in Onion (Allium cepa L.)

N. Joshi, A. Ravindran and V. Mahajan

Genetic improvement of onion (Allium cepa L.) by conventional methods of hybridization is slow due to its highly heterozygous, outcrossing and biennial nature. To augment this process induced mutagenesis could serve as a useful tool. Keeping this in view, the present study was conducted to test the efficacy of Ethyl Methane Sulphonate (EMS) and Sodium Azide (SA) in inducing variability in onion. For this purpose, seeds of six onion varieties were soaked in solutions of different concentrations of EMS (0.1, 0.15, 0.2, 0.25%) and SA (0.1, 0.3, 0.5, 0.7%) for 4 h and their sensitivity towards these mutagens was assessed in terms of seed germination and seedling growth. Significant differences (p<0.01) were observed in the varieties and treatments with respect to the traits studied. Lower dose (0.1%) of both SA and EMS showed stimulatory effect on germination potential of seeds of all except variety-4 and variety-5. Shoot length was enhanced over control in variety-3 and variety-5 at 0.1% SA and variety-4 at 0.1% EMS. Root length was slightly higher than control in variety-3 and variety-4 at 0.1% SA and 0.1% EMS, respectively. Reduction in all the three traits was observed at higher doses of EMS and SA in all the varieties. The reduction in shoot and root length was more pronounced under SA treatment than EMS. Also, germination process was more sensitive to mutagens than the seedling growth. The present study demonstrated that both mutagens at low dose could be suitable for the creation of variability in onion.
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  •    Effect of Dimethyl Sulphate on the Growth, Induction of Mutations and Their Identification by Peroxidase Isozyme in Tecoma stans
  •    Effect of some Chemical Mutagens on the Growth, Phytochemical Composition and Induction of Mutations in Khaya senegalensis
  •    Induction of Mutations in Celosia argentea using Dimethyl Sulphate and Identification of Genetic Variation by ISSR Markers
  •    Variability and Correlations Studies for Total Iron and Manganese Contents of Chickpea (Cicer arietinum L.) High Yielding Mutants
  •    Induced Mutagenesis in Urdbean (Vigna mungo L. Hepper): A Review
  •    Chromosomal Aberrations Induced by Chemical Mutagens in Allium
  •    Effect of Gamma Rays and EMS on Two Varieties of Soybean
  •    EMS Induced Mutational Variability in Vigna radiata and Vigna mungo
How to cite this article:

N. Joshi, A. Ravindran and V. Mahajan, 2011. Investigations on Chemical Mutagen Sensitivity in Onion (Allium cepa L.). International Journal of Botany, 7: 243-248.

DOI: 10.3923/ijb.2011.243.248






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