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Asian Journal of Crop Science
  Year: 2020 | Volume: 12 | Issue: 2 | Page No.: 90-96
DOI: 10.3923/ajcs.2020.90.96
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Determination of Caffeine Content in Robusta Roasted Coffee (Coffea canephora) by RP-UHPLC-PDA

Juan Gaibor, Dayana Morales and Wilman Carrillo

Background and Objective: Coffea canephora (named Robusta coffee) represent around the 30% of the production worldwide of coffee. This specie is used as coffee beverage. Their content of caffeine is important in their quality. The aim of this study was to determine the caffeine content in robusta coffee (Coffea canephora) variety obtained from 8 cultivars (Piedra Grande, Sabanetillas, Pitiamby, Industria Tablas de Florida, Yatuví, Pueblo Nuevo and Paraiso) of 2 Cantons (Caluma and Echeandía) of the Bolívar province from Ecuador. Materials and Methods: Caffeine content of robusta coffee (Coffea canephora) from Ecuador was calculated using the reverse phase ultra-high-performance liquid chromatography RP-UHPLC-PDA with the help of caffeine standard using a C18 column and lineal gradient of 0-70% B for 12 min with an absorbance of 274 nm (solvent B: Acetonitrile and solvent A: Water). Caffeine content of robusta coffee from Ecuador was also evaluated with the extraction soxhlet method. Results: It was found that three of these Ethiopian plants (designated here as UEB-F, UEB-Y and UEB-P in honor to their localization) had an almost low caffeine content as the Coffea canephora content. This Coffea canephora plant was detected during the analysis of ultra- high-performance liquid chromatography (UHPLC-PDA) of chloroform extracts of seeds. Conclusion: Robusta coffee (Coffea canephora) cultivated in the Echeandía canton present an extremely low value of caffeine content. These values of caffeine allow this coffee variety to be used to produce decaffeinated coffee. Caluma canton coffee presents caffeine content according to the robusta coffee variety.
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How to cite this article:

Juan Gaibor, Dayana Morales and Wilman Carrillo, 2020. Determination of Caffeine Content in Robusta Roasted Coffee (Coffea canephora) by RP-UHPLC-PDA. Asian Journal of Crop Science, 12: 90-96.

DOI: 10.3923/ajcs.2020.90.96






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