Asian Journal of Crop Science1994-78792077-2041Asian Network for Scientific Information10.3923/ajcs.2020.90.96Coffea canephora) by RP-UHPLC-PDA]]>GaiborJuan MoralesDayana CarrilloWilman 22020122Background and Objective:Coffea canephora (named Robusta coffee) represent around the 30% of the production worldwide of coffee. This specie is used as coffee beverage. Their content of caffeine is important in their quality. The aim of this study was to determine the caffeine content in robusta coffee (Coffea canephora) variety obtained from 8 cultivars (Piedra Grande, Sabanetillas, Pitiamby, Industria Tablas de Florida, Yatuví, Pueblo Nuevo and Paraiso) of 2 Cantons (Caluma and Echeandía) of the Bolívar province from Ecuador. Materials and Methods: Caffeine content of robusta coffee (Coffea canephora) from Ecuador was calculated using the reverse phase ultra-high-performance liquid chromatography RP-UHPLC-PDA with the help of caffeine standard using a C18 column and lineal gradient of 0-70% B for 12 min with an absorbance of 274 nm (solvent B: Acetonitrile and solvent A: Water). Caffeine content of robusta coffee from Ecuador was also evaluated with the extraction soxhlet method. Results: It was found that three of these Ethiopian plants (designated here as UEB-F, UEB-Y and UEB-P in honor to their localization) had an almost low caffeine content as the Coffea canephora content. This Coffea canephora plant was detected during the analysis of ultra- high-performance liquid chromatography (UHPLC-PDA) of chloroform extracts of seeds. Conclusion: Robusta coffee (Coffea canephora) cultivated in the Echeandía canton present an extremely low value of caffeine content. These values of caffeine allow this coffee variety to be used to produce decaffeinated coffee. Caluma canton coffee presents caffeine content according to the robusta coffee variety.]]>Cagliani, L.R., G. Pellegrino, G. Giugno and R. Consonni,2013Coffea arabica and Coffea canephora var. robusta in roasted and ground coffee blends.]]>106169173Burns, D.T., L. Tweed and M.J. Walker,20171023022310Bhaktha, G., M. Shantaram and S. Nayak,20168393395De Carvalho, K., C.L.O. Petkowicz, G.T. Nagashima, J.C.B. Filho, L.G.E. Vieira, L.F.P. Pereira and D.S. Domingues,2014Coffea Arabica.]]>289951963Babova, O., A. Occhipinti and M.E. Maffei,2016Coffea arabica and Coffea canephora) of different geographical origin.]]>1233339Dankowska, A., A. Domagala and W. Kowalewski,2017Coffea arabica and Coffea canephora var. robusta concentration in blends by means of synchronous fluorescence and UV-Vis spectroscopies.]]>172215220Bicho, N.C., A.E. Leitao, J.C. Ramalho, N.B. de Alvarenga and F.C. Lidon,201316895904Panusa, A., R. Petrucci, R. Lavecchia and A. Zuorro,201799155165Bresciani, L., L. Calani, R. Bruni, F. Brighenti and D. Del Rio,201461196201Alomar, M.J.,20168101103Pizarro, C., I. Esteban-Diez and J.M. Gonzalez-Saiz,2007Robusta variety in order to detect adulteration in roasted coffee by near infrared spectroscopy.]]>585266276Santos, J.R., M. Lopo, A.O.S.S. Rangel and J.A. Lopes,201660408415Calvini, R., A. Ulrici and J.M. Amigo,2015146503511Carrera, F., M. Leon-Camacho, F. Pablos and A.G. Gonzalez,1998370131139Martin, M.J., F. Pablos, A.G. Gonzalez, M.S. Valdenebro and M. Leon-Camacho,200154291297Wei, X., F. Liu, Z. Qiu, Y. Shao and Y. He,2014713711380Aquino, F.J., R. Augusti, J.D.O. Alves, M.E.R. Diniz and S.A.L. Morais et al.,2014117127132Pauli, E.D., F. Barbieri, P.S. Garcia, T.B. Madeira and V.R. Acquaro Jr. et al.,201461112119Mehari, B., M. Redi-Abshiro, B.S. Chandravanshi, S. Combrinck, M. Atlabachew and R. McCrindle,2016451625Vochyanova, B., F. Opekar and P. Tuma,20143516601665Yanes, E.G., S.R. Gratz and A.M. Stalcup,200012519191923De Melo, M.M.R., H.M.A. Barbosa, C.P. Passos and C.M. Silva,201486150159McCusker, R.R., B.A. Goldberger and E.J. Cone,200327520522Adam, D.J., J. Mainwaring and M.N. Quigley,19967311711171Lara, D., E. Vilcacundo, C. Carrillo, M. Silva, M. Alvarez and, W. Carrillo,2017Macadamia intergrifolia) with aqueous extraction method.]]>10138142Panusa, A., A. Zuorro, R. Lavecchia, G. Marrosu and R. Petrucci,20136141624168Burns, D.T. and M.J. Walker,20192019Joet, T., A. Laffargue, F. Descroix, S. Doulbeau, B. Bertrand, A. de Kochko and S. Dussert,2010118693701Kitzberger, C.S.G., M.B. dos Santos Scholz, L.F.P. Pereira, L.G.E. Vieira, T. Sera, J.B.G.D. Silva and M. de Toledo Benassi,2013Coffea arabica cultivars growing in the same edapho-climatic conditions.]]>305257El-Abassy, R.M., P. Donfack and A. Materny,201112614431448Campa, C., S. Doulbeau, S. Dussert, S. Hamon and M. Noirot,2005Coffea species: Evidence of a discontinuous distribution.]]>91633637Hecimovic, I., A. Belscak-Cvitanovic, D. Horzic and D. Komes,20111299911000