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Asian Journal of Biochemistry

Year: 2007 | Volume: 2 | Issue: 6 | Page No.: 427-431
Research Article

Isolation and evaluation of Endonucleases in apoptotic activity of DNA in Ischemic Injury and Reperfusion Injury of Rat Hepatocytes

P. Chattopadhyay
Birla Institute Technology and Sciences, Pilani-333031, Rajasthan, India National Biotechnology Centre, Indian Veterinary Research Institute, Izatnagar-243122, UP, India
G. Sukla
Microbiology and Cellular Biology Laboratory, College of Pharmacy, IFTM, Lodhipur Rajput, Moradabad-24001, UP, India
R. Gautam
Microbiology and Cellular Biology Laboratory, College of Pharmacy, IFTM, Lodhipur Rajput, Moradabad-24001, UP, India
L. Singh
Microbiology and Cellular Biology Laboratory, College of Pharmacy, IFTM, Lodhipur Rajput, Moradabad-24001, UP, India
A. Verma
Microbiology and Cellular Biology Laboratory, College of Pharmacy, IFTM, Lodhipur Rajput, Moradabad-24001, UP, India
N. Verma
Microbiology and Cellular Biology Laboratory, College of Pharmacy, IFTM, Lodhipur Rajput, Moradabad-24001, UP, India
S. Kumar
Microbiology and Cellular Biology Laboratory, College of Pharmacy, IFTM, Lodhipur Rajput, Moradabad-24001, UP, India
A.K. Wahi
Microbiology and Cellular Biology Laboratory, College of Pharmacy, IFTM, Lodhipur Rajput, Moradabad-24001, UP, India
The internucleosomal cleavage of genomic DNA is a biochemical hallmark of apoptosis. DNase endonuclease has been suggested to be one of the apoptotic endonuclease, but its biochemical characteristic has not been fully elucidated in ischemic and reperfusion (IR) injury. The present study suggests that DNase-I is involved in the apoptosis of IR injured rat liver The finding suggests a possible biological basis of endonuclease associated apoptotic effects of DNA in ischemic and reperfused hepatocytes.
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How to cite this article

P. Chattopadhyay, G. Sukla, R. Gautam, L. Singh, A. Verma, N. Verma, S. Kumar and A.K. Wahi, 2007. Isolation and evaluation of Endonucleases in apoptotic activity of DNA in Ischemic Injury and Reperfusion Injury of Rat Hepatocytes. Asian Journal of Biochemistry, 2: 427-431.

DOI: 10.3923/ajb.2007.427.431

URL: https://scialert.net/abstract/?doi=ajb.2007.427.431

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