Putu Ari Agus Pawartha
Department of Community Nutrition, Faculty of Human Ecology, Bogor Agricultural University, Bogor-16680, Indonesia
Rimbawan
Department of Community Nutrition, Faculty of Human Ecology, Bogor Agricultural University, Bogor-16680, Indonesia
Ikeu Tanziha
Department of Community Nutrition, Faculty of Human Ecology, Bogor Agricultural University, Bogor-16680, Indonesia
Wiwin Winarsih
Department of Veterinary Clinic Reproduction and Pathology, Bogor Agricultural University, Bogor-16680, Indonesia
Sri Usmiati
Indonesian Center for Agricultural Postharvest Research and Development, Indonesia
ABSTRACT
The aim of this study was to evaluate the effectiveness of encapsulated Lactobacillus casei probiotics isolated from dadih (West Sumatra traditional yoghurt) against diarrhea caused by Escherichia coli. Total 30 male Sprague-dawley rats were divided into five groups (1) normal control rats, (2) infected rats, (3) normal rats with encapsulated probiotics, (4) infected rats with encapsulated probiotics, (5) infected rats with encapsulated probiotics given simultaneously. Rats were fed with standard diet and aquades ad libitum. Infected rats were challenged by E. coli (108 cfu/ml) for 7 days daily orally and then administered by 109 cfu/g probiotics for the next 7 days. On day 0, 3 and 7, total of fecal lactic acid bacteria (LAB) and E. coli were evaluated. Feed intake, weight gain and food conversion efficiency (FCE) were also evaluated. At the end of treatment rats were sacrificed to observed goblet cells count obtained from ileum. It was observed that encapsulated Lactobacillus casei could increase FCE and total LAB. Encapsulated Lactobacillus casei also could reduce E. coli population and reduce total goblet cells on infected rats. Encapsulated Lactobacillus casei has potential effect as probiotics against E. coli on rats even it is not statistically different.
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How to cite this article
Putu Ari Agus Pawartha, Rimbawan, Ikeu Tanziha, Wiwin Winarsih and Sri Usmiati, 2015. Efficacy of Encapsulated Lactobacillus casei Probiotics as Anti Diarrheal Agent on Sprague Dawley Rats. Pakistan Journal of Nutrition, 14: 666-671.
DOI: 10.3923/pjn.2015.666.671
URL: https://scialert.net/abstract/?doi=pjn.2015.666.671
DOI: 10.3923/pjn.2015.666.671
URL: https://scialert.net/abstract/?doi=pjn.2015.666.671
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