Achmad Noerkhaerin Putra
Fisheries Study Program, Faculty of Agriculture, Sultan Ageng Tirtayasa University,
Nur Bambang Priyo Utomo
Department of Aquaculture, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University, Jalan Agatis, Dramaga Campus, Bogor-16680, West Java, Indonesia
Widanarni
Department of Aquaculture, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University, Jalan Agatis, Dramaga Campus, Bogor-16680, West Java, Indonesia
ABSTRACT
The aim of this study was to evaluate the growth performance of tilapia which were given probiotic, prebiotic and synbiotic through feed. The probiotic bacteria used was selected from Np 1, Np 3 and Np 5. The prebiotic was extracted from sweet potato var. sukuh, while the synbiotic was a combination between a probiotic and prebiotic. This study was conducted in two phases, the in vitro probiotics and prebiotics synergism test and the in vivo feeding trial of selected probiotic, prebiotic and synbiotic to tilapia. The in vivo assays had four treatments with three replications, i.e., (A) control, (B) probiotic 1% (v/w), (C) prebiotic 2% (v/w) and (D) synbiotic (probiotic 1% (v/w)+prebiotic 2% (v/w)). Results of the in vitro assays showed that the three probiotic isolates could grow in media containing the prebiotics and Np 5 isolate demonstrated the best growth. In the in vivo assays, the application of the synbiotic resulted in the best growth rate, feed efficiency, digestive enzyme activity, feed digestibility and nutrient retention compared to the control and the other treatments.
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How to cite this article
Achmad Noerkhaerin Putra, Nur Bambang Priyo Utomo and Widanarni, 2015. Growth Performance of Tilapia (Oreochromis niloticus) Fed with Probiotic, Prebiotic and Synbiotic in Diet. Pakistan Journal of Nutrition, 14: 263-268.
DOI: 10.3923/pjn.2015.263.268
URL: https://scialert.net/abstract/?doi=pjn.2015.263.268
DOI: 10.3923/pjn.2015.263.268
URL: https://scialert.net/abstract/?doi=pjn.2015.263.268
REFERENCES
- Ai, Q., H. Xu, K. Mai, W. Xu, J. Wang and W. Zhang, 2011. Effects of dietary supplementation of Bacillus subtilis and fructooligosaccharide on growth performance, survival, non-specific immune response and disease resistance of juvenile large yellow croaker, Larimichthys crocea. Aquaculture, 317: 155-161.
CrossRefDirect Link - Akrami, R., Y. Iri, H.K. Rostami and M.R. Mansour, 2013. Effect of dietary supplementation of fructooligosaccharide (FOS) on growth performance, survival, lactobacillus bacterial population and hemato-immunological parameters of stellate sturgeon (Acipenser stellatus) juvenile. Fish Shellfish Immunol., 35: 1235-1239.
CrossRefDirect Link - Cerezuela, R., J. Meseguer and M.A. Esteban, 2011. Current knowledge in synbiotic use for fish aquaculture: A review. J. Aquacult. Res. Dev.
CrossRefDirect Link - Daniels, C.L., D.L. Merrifield, D.P. Boothroyd, S.J. Davies, J.R. Factor and K.E. Arnold, 2010. Effect of dietary Bacillus spp. and mannan oligosaccharides (MOS) on European lobster (Homarus gammarus L.) larvae growth performance, gut morphology and gut microbiota. Aquaculture, 304: 49-57.
CrossRefDirect Link - Delgado, G.T.C., W.M.S.C. Tamashiro and G.M. Pastore, 2010. Immunomodulatory effects of fructans. Food Res. Int., 43: 1231-1236.
CrossRefDirect Link - Delgado, G.T.C., W.M. da Silva Cunha Tamashiro, M.R. Marostica Jr., Y.M.F. Moreno and G.M. Pastore, 2011. The putative effects of prebiotics as immunomodulatory agents. Food Res. Int., 44: 3167-3173.
CrossRefDirect Link - Geraylou, Z., C. Souffreau, E. Rurangwa, L. De Meester and C.M. Courtin et al., 2013. Effects of dietary arabinoxylan-oligosaccharides (AXOS) and endogenous probiotics on the growth performance, non-specific immunity and gut microbiota of juvenile Siberian sturgeon (Acipenser baerii). Fish Shellfish Immunol., 35: 766-775.
CrossRefDirect Link - Grisdale-Helland, B., S.J. Helland and D.M. Gatlin III, 2008. The effects of dietary supplementation with mannanoligosaccharide, fructooligosaccharide or galactooligosaccharide on the growth and feed utilization of Atlantic salmon (Salmo salar). Aquaculture, 283: 163-167.
CrossRefDirect Link - Hernandez-Hernandez, O., A. Muthaiyan, F.J. Moreno, A. Montilla, M.L. Sanz and S.C. Ricke, 2012. Effect of prebiotic carbohydrates on the growth and tolerance of Lactobacillus. Food Microbiol., 30: 355-361.
CrossRefDirect Link - Hoseinifar, S.H., M. Khalili, H.K. Rostami and M.A. Esteban, 2013. Dietary galactooligosaccharide affects intestinal microbiota, stress resistance and performance of Caspian roach (Rutilus rutilus) fry. Fish Shellfish Immunol., 35: 1416-1420.
CrossRefDirect Link - Kesarcodi-Watson, A., H. Kaspar, M.J. Lategan and L. Gibson, 2008. Probiotics in aquaculture: The need, principles and mechanisms of action and screening processes. Aquaculture, 274: 1-14.
CrossRefDirect Link - Li, J., B. Tan and K. Mai, 2009. Dietary probiotic Bacillus OJ and isomaltooligosaccharides influence the intestine microbial populations, immune responses and resistance to white spot syndrome virus in shrimp (Litopenaeus vannamei). Aquaculture, 291: 35-40.
CrossRefDirect Link - Li, P., G.S. Burr, D.M. Gatlin III, M.E. Hume, S. Patnaik, F.L. Castille and A.L. Lawrence, 2007. Dietary supplementation of short-chain Fructooligosaccharides influences Gastrointestinal Microbiota composition and immunity characteristics of Pacific White Shrimp, Litopenaeus vannamei, cultured in a recirculating system. J. Nutr., 137: 2763-2768.
Direct Link - Lin, S., S. Mao, Y. Guan, L. Luo, L. Luo and Y. Pan, 2012. Effects of dietary chitosan oligosaccharides and Bacillus coagulans on the growth, innate immunity and resistance of koi (Cyprinus carpio koi). Aquaculture, 342-343: 36-41.
CrossRefDirect Link - Mahious, A.S., F.J. Gatesoupe, M. Hervi, R. Metailler and F. Ollevier, 2006. Effect of dietary inulin and oligosaccharides as prebiotics for weaning turbot, Psetta maxima (Linnaeus, C. 1758). Aquacult. Int., 14: 219-229.
CrossRefDirect Link - Merrifield, D.L., A. Dimitroglou, A. Foey, S.J. Davies and R.T.M. Baker et al., 2010. The current status and future focus of probiotic and prebiotic applications for salmonids. Aquaculture, 302: 1-18.
CrossRefDirect Link - Apun-Molina, J.P., A. Santamaria-Miranda, A. Luna-Gonzalez, S.F. Martinez-Diaz and M. Rojas-Contreras, 2009. Effect of potential probiotic bacteria on growth and survival of tilapia Oreochromis niloticus L., cultured in the laboratory under high density and suboptimum temperature. Aquacult. Res., 40: 887-894.
CrossRefDirect Link - Ringo, E., R.E. Olsen, T.O. Gifstad, R.A. Dalmo, H. Amlund, G.I. Hemre and A.M. Bakke, 2010. Prebiotics in aquaculture: A review. Aquacult. Nutr., 16: 117-136.
CrossRefDirect Link - Soleimani, N., S.H. Hoseinifar, D.L. Merrifield, M. Barati and Z.H. Abadi, 2012. Dietary supplementation of fructooligosaccharide (FOS) improves the innate immune response, stress resistance, digestive enzyme activities and growth performance of Caspian roach (Rutilus rutilus) fry. Fish Shellfish Immunol., 32: 316-321.
CrossRefDirect Link - Taoka, Y., H. Maeda, J.Y. Jo and T. Sakata, 2007. Influence of commercial probiotics on the digestive enzyme activities of Tilapia, Oreochromis niloticus. Aquacult. Sci., 55: 183-189.
CrossRefDirect Link - Vernazza, C.L., G.R. Gibson and R.A. Rastall, 2006. Carbohydrate preference, acid tolerance and bile tolerance in five strains of Bifidobacterium. J. Applied Microbiol., 100: 846-853.
CrossRefDirect Link - Wang, Y.B., 2007. Effect of probiotics on growth performance and digestive enzyme activity of the shrimp Penaeus vannamei. Aquaculture, 269: 259-264.
CrossRefDirect Link - Zhang, J., Y. Liu, L. Tian, H. Yang, G. Liang and D. Xu, 2012. Effects of dietary mannan oligosaccharide on growth performance, gut morphology and stress tolerance of juvenile Pacific white shrimp, Litopenaeus vannamei. Fish Shellfish Immunol., 33: 1027-1032.
CrossRefPubMedDirect Link - Zhang, Q., H. Ma, K. Mai, W. Zhang, Z. Liufu and W. Xu, 2010. Interaction of dietary Bacillus subtilis and fructooligosaccharide on the growth performance, non-specific immunity of sea cucumber, Apostichopus japonicus. Fish Shellfish Immunol., 29: 204-211.
CrossRefDirect Link - Zhou, Q.C., J.A. Buentello and D.M. Gatlin III, 2010. Effects of dietary prebiotics on growth performance, immune response and intestinal morphology of red drum (Sciaenops ocellatus). Aquaculture, 309: 253-257.
CrossRefDirect Link - Zokaeifar, H., J.L. Balcazar, C.R. Saad, M.S. Kamarudin, K. Sijam, A. Arshad and N. Nejat, 2012. Effects of Bacillus subtilis on the growth performance, digestive enzymes, immune gene expression and disease resistance of white shrimp, Litopenaeus vannamei. Fish Shellfish Immunol., 33: 683-689.
CrossRefPubMedDirect Link