Enny Purwati Nurlaili
Faculty of Agricultural Technology, Universitas 17 Agustus 1945 Semarang, Jawa Tengah, Indonesia
Mary Astuti
Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia
Yustinus Marsono
Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia
Sri Naruki
Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia
ABSTRACT
Rice is a leading staple food in Southeast Asia and it is typically milled before consumption. Black rice found in Indonesia is classified as Oryza sativa L. The color in the grain is caused by anthocyanin pigments that give the hulled rice a dark purple color. We found that extracts from black rice bran have high levels of iron and anthocyanins. The objective of this study was to evaluate the potential of an aqueous extract of black rice bran to prevent anemia and hypertriglyceridemia in vivo. Anemia and hypertriglyceridemia were induced in twenty-eight three-week old male albino Wistar rats (Rattus norvegicus); the rats were divided into a control (C) group or 3 treatment groups of bran (B), bran extract (E), or extraction residue (R). The results showed that differences among the groups based on feed consumption, Hemoglobin Regeneration Efficiency (HRE) and the erythrocyte morphology of rats were not significant. Black rice bran aqueous extract prevented anemia and hypertriglyceridemia by increasing the hemoglobin Hb level from 7.21 to 12.96 g/dl and by reducing triglycerides from 179.29 to 56.55 mg/dl.
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How to cite this article
Enny Purwati Nurlaili, Mary Astuti, Yustinus Marsono and Sri Naruki, 2016. An Aqueous Extract of Black Rice Bran from the Cibeusi Variety Prevents Anemia and Hypertriglyceridemia in Rats. Pakistan Journal of Nutrition, 15: 837-845.
DOI: 10.3923/pjn.2016.837.845
URL: https://scialert.net/abstract/?doi=pjn.2016.837.845
DOI: 10.3923/pjn.2016.837.845
URL: https://scialert.net/abstract/?doi=pjn.2016.837.845
REFERENCES
- Brownlie, T., V. Utermohlen, P.S. Hinton and J.D. Haas, 2004. Tissue iron deficiency without anemia impairs adaptation in endurance capacity after aerobic training in previously untrained women. Am. J. Clin. Nutr., 73: 437-443.
Direct Link - Choi, J.W., S.K. Kim and S.H. Pai, 2001. Changes in serum lipid concentrations during iron depletion and after iron supplementation. Ann. Clin. Laboratory Sci., 31: 151-156.
Direct Link - Fields, M. and C.G. Lewis, 1999. Cholesterol-lowering nature of unsaturated fat in rats may be due to its inability to increase hepatic iron. Metabol. Clin. Exp., 48: 200-204.
CrossRefDirect Link - Ghiselli, A., M. Nardini, A. Baldi and C. Scaccini, 1998. Antioxidant activity of different phenolic fractions separated from an Italian red wine. J. Agric. Food Chem., 46: 361-367.
CrossRefDirect Link - Gregorio, G.B., D. Senadhira, T. Htut and R.D. Graham, 1999. Improving iron and zinc value of rice for human nutrition. Agriculture et Developpement, 23: 77-81.
Direct Link - Haas, J.D. and T. Brownlie, 2001. Iron deficiency and reduced work capacity: A critical review of the research to determine a causal relationship. J. Nutr., 131: 676S-690S.
Direct Link - Hernandez, M., V. Sousa, A. Moreno, S. Villapando and M. Lopez-Alarcon, 2003. Iron bioavailability and utilization in rats are lower from lime-treated corn flour than from wheat flour when they are fortified with different sources of iron. J. Nutr., 133: 154-159.
PubMedDirect Link - Kaneda, I., H. Yasui, Y. Adachi, J. Takada and H. Sakurai, 2005. Determination of trace element concentrations in ancient rices (red and black rices) and a present-day rice (Koshihikari): Relationship among the trace element concentrations, species, harvest site and rice parts. Biomed. Res. Trace Elements, 16: 241-249.
CrossRefDirect Link - Kaneda, I., F. Kubo and H. Sakurai, 2007. Relationship between trace metal concentration and antioxidative activity of ancient rice bran (red and black rice) and a present-day rice bran (Koshihikari). J. Trace Element Med. Biol., 21: 45-51.
CrossRefDirect Link - Kim, J., X. Jia, P.D. Buckett, S. Liu, C.H. Lee and M. Wessling-Resnick, 2013. Iron loading impairs lipoprotein lipase activity and promotes hypertriglyceridemia. FASEB J., 27: 1657-1663.
CrossRefDirect Link - Kong, S. and J. Lee, 2010. Antioxidants in milling fractions of black rice cultivars. Food Chem., 120: 278-281.
CrossRefDirect Link - Meng, F., Y. Wei and X. Yang, 2005. Iron content and bioavailability in rice. J. Trace Elem. Med. Biol., 18: 333-338.
CrossRefPubMedDirect Link - Mpiana, P.T., K.N. Ngbolua, M.T. Bokota, T.K. Kasonga, E.K. Atibu, D.S. Tshibangu and V. Mudogo, 2010. In vitro effects of anthocyanin extracts from Justicia secunda VAHL on the solubility of haemoglobin S and membrane stability of sickle erythrocytes. Blood Transfus., 8: 248-254.
CrossRefPubMedDirect Link - Ono, K., N. Sugihara, Y. Hirose and K. Katagiri, 2003. An examination of optimal extraction solvents for anthocyanin pigments from black rice produced in Gifu. J. Gifu City Women's College, 52: 135-138, (In Japanese).
Direct Link - Provan, D., 1999. Mechanisms and management of iron deficiency anaemia. Br. J. Haematol., 105: 19-26.
PubMedDirect Link - Reeves, P.G., F.H. Nielsen and G.C. Fahey Jr., 1993. AIN-93 purified diets for laboratory rodents: Final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J. Nutr., 123: 1939-1951.
CrossRefPubMedDirect Link - Stoltzfus, R.J., J.D. Kvalsvig, H.M. Chwaya, A. Montresor and M. Albonico, 2002. Effects of iron supplementation and anthelmintic treatment on motor and language development of preschool children in Zanzibar: Double blind, placebo controlled study. Br. Med. J., 323: 1389-1393.
CrossRefDirect Link - Tedesco, I., G.L. Russo, F. Nazzaro, M. Russo and R. Palumbo, 2001. Antioxidant effect of red wine anthocyanins in normal and catalase-inactive human erythrocytes. J. Nutr. Biochem., 12: 505-511.
CrossRefDirect Link - Wu, A.C., L. Lesperance and H. Bernstein, 2002. Screening for iron deficiency. Pediatr. Rev., 23: 171-177.
Direct Link - Yamagishi, H., H. Okazaki, M. Shimizu, T. Izawa and T. Komabayashi, 2000. Relationships among serum triacylglycerol, fat pad weight and lipolysis in iron-deficient rats. J. Nutr. Biochem., 11: 455-460.
CrossRefDirect Link