Subscribe Now Subscribe Today
Research Article
 

Enzyme Activities and Histology Study on High Fat Diet-induced Obese Rats by Pink Guava Puree



M.N. Norazmir, M.Y. Ayub and M.M.A. Ummi
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail
ABSTRACT

The effects of pink guava (Psidium guajava) puree on enzyme activities and histology on High Fat Diet (HFD)-induced obese rats were investigated. Thirty male Sprague-Dawley rats were divided into Control Negative (CN) fed with rat pellet; control positive, low, medium and high dose group (CP, LDG, MDG and HDG) were fed HFD-AIN93G, respectively. CN and CP were given distilled water; meanwhile treated group were given the aqueous puree, at concentration of 500, 1000 and 2000 mg/kg body weight, dissolved in distilled water were administered orally via a drinking bottle, respectively. Pink guava puree was supplemented with the HFD diet for six weeks. The rats were fasted overnight and euthanized under an anesthetic condition with ethyl ether and blood was collected from the posterior vena cava at the end of experiment. A significant reduction in body weight was observed in the treated groups as compared to CN and CP group. Specific activities of Glutathione Peroxidase (GPx), Glutathione Reductase (GR) and Superoxide Dismutase (SOD) of the HFD-induced obese rats were significantly increased in comparison with the CN group. Histologically, the liver and kidney cells in LDG, MDG and HDG showed no significant differences as compared to CN and CP’s liver and kidney cells. Treatment with low, medium and high doses showed improved features in HFD induced-obese rat’s liver and kidney cells. In conclusion, pink guava puree due to its antioxidant role was helpful in protecting organ tissues in experimental animals and has a significant impact on specific activities of HFD induced-obese rats.

Services
Related Articles in ASCI
Similar Articles in this Journal
Search in Google Scholar
View Citation
Report Citation

 
  How to cite this article:

M.N. Norazmir, M.Y. Ayub and M.M.A. Ummi, 2010. Enzyme Activities and Histology Study on High Fat Diet-induced Obese Rats by Pink Guava Puree. Pakistan Journal of Nutrition, 9: 1100-1106.

DOI: 10.3923/pjn.2010.1100.1106

URL: https://scialert.net/abstract/?doi=pjn.2010.1100.1106

REFERENCES
1:  Appel, L.J., T.J. Moore and E. Obarzanek, 1997. A clinical trial of the effects of dietary patterns on blood pressure. N. Engl. J. Med., 336: 1117-1124.
Direct Link  |  

2:  Asmah, R., F.A.B. Mohd and H. Zarida, 2006. The effects of guava (Psidium guava) consumption on total antioxidant and lipid profile in normal male youth. Afr. J. Food. Agric. Nutr. Dev., 6: 1-12.
Direct Link  |  

3:  Ayub, M.Y., M.N. Norazmir, S. Mamot, K. Jeeven and H. Hadijah, 2010. Anti-hypertensive effect of pink guava (Psidium guajava) puree on spontaneous hypertensive rats. Int. Food Res. J., 17: 89-96.
Direct Link  |  

4:  Brzoska, M.M., J.M. Jakoniuk, B.P. Marcinkiewicz and B. Sawicki, 2003. Liver and kidney function and histology in rats exposed to cadmium and ethanol. Alcohol Alcoholism., 38: 2-10.
CrossRef  |  

5:  Cadenas, E., A. Boveris and C.I. Ragan, 1997. Production of superoxide radical and hydrogen peroxide by NADH-ubiqunine reductase and ubiquinol-cytochrome c reductase from beef heart mitochondria. Arch. Biochem. Biophys., 180: 248-257.

6:  Datta, S., S. Sinha and P. Bhattacharyya, 1999. Effect of an herbal protein, CI-I, purified from Cajanus indicus, in models of liver failure in mice. Drug Dev. Res., 48: 76-83.

7:  Dobrian, A.D., M.J. Davies, R.L. Prewitt and T.J. Lauterio, 2000. Development of hypertension in a rat model of diet-induced obesity. Hypertension, 35: 1009-1015.
PubMed  |  

8:  Herrera-Arellano, A., S. Flores-Romero, M.A. Chavez-Soto and J. Tortorie-Ilo, 2004. Effectiveness and tolerability of a standardized extract from Hibiscus sabdariffa in patients with mild to moderate hypertension: A controlled and randomized clinical trial. Phytomedicine, 5: 375-382.
CrossRef  |  PubMed  |  Direct Link  |  

9:  Hughes, J. and A. Jefferson, 2008. Clinical Chemistry. Elsevier Science, Churchill, Livingstone.

10:  Humason, G.L., 1979. Animal Tissue Techniques. W.H. Freeman and Co., San Francisco.

11:  James, P.T., 2004. Obesity: The worldwide epidemic. Clin. Dermatol., 22: 276-280.
PubMed  |  

12:  Kadiri, S., A. Arije and B.L. Salako, 1999. Tradititional herbal preparations and acute renal failure in South West Nigeria. Trop. Doc., 29: 244-246.
PubMed  |  

13:  Lahlou, S., A. Tahraoui, Z. Israili and B. Lyoussi, 2007. Diuretic activity of the aqueous extracts of Carum carvi and Tanacetum vulgare in normal rats. J. Ethnopharmacol., 110: 458-463.
PubMed  |  Direct Link  |  

©  2020 Science Alert. All Rights Reserved