Subscribe Now Subscribe Today
Fulltext PDF
Research Article
The Effect of Neem (Azadirachta indica) Aqueous Extract and Dietary Selenium on Distribution of Selenium in Liver Tissue During Hepatocarcinogenesis

P. Hanachi , O. Fauziah , L.L. Nam , T.S. Tian , L.T. Peng , L.C. Weil and O.K. Ho

This study aimed to determine the distribution of selenium in the liver of rats during hepatocarcinogenesis when neem aqueous extract and dietary selenium was supplemented. The selenium distribution of the 9 diethylnitrosamine (DEN) and 2-acetylaminofluorene (2-AAF) induced hepatocarcinogenesis in Sprague dawley male rats as well as the other 9 normal rats, which were supplemented with 5% (w/v) neem leaves aqueous extract, 1.85 mg L-1 sodium selenate and tap water ad libitum according to the treatment groups for 10 weeks, were assessed with energy filter transmission electron microscope (EFTEM) by electron spectroscopic imaging (ESI)-3 window power law method. The result showed that the selenium distribution mean score in normal control group was the lowest and cancerous control group was the highest. However, the selenium distribution mean score in normal supplemented with 5% (w/v) neem leaves aqueous extract increased about 26% (p<0.05) compared to normal control group. This study suggested that neem leaves aqueous extract may be a potential primary chemopreventive agent rather than as secondary chemopreventive agent.

Related Articles in ASCI
Similar Articles in this Journal
Search in Google Scholar
View Citation
Report Citation

  How to cite this article:

P. Hanachi , O. Fauziah , L.L. Nam , T.S. Tian , L.T. Peng , L.C. Weil and O.K. Ho , 2004. The Effect of Neem (Azadirachta indica) Aqueous Extract and Dietary Selenium on Distribution of Selenium in Liver Tissue During Hepatocarcinogenesis . Journal of Biological Sciences, 4: 584-587.

DOI: 10.3923/jbs.2004.584.587


Bosch, F.X., J. Ribes and J. Borras, 1999. Epidemiology of primary liver cancer. Seminar Liver Dis., 19: 271-285.
CrossRef  |  PubMed  |  

Conney, A.H., Z.Y. Wang, M.T. Huang, C.T. Ho and C.S. Yang, 1992. Inhibitory Effect of Oral Administration of Green Tea on Tumorigenesis by Ultraviolet Light, 12-0-Tetradecanoylphorbol-13-Acetate and N-Nitrosodiethylamine in Mice. In: Cancer Chemoprevention, Lee, W., L. Martin, B.W. Charles and K. Gary (Eds.). CRC Press, Inc., New York, pp: 362.

De Jorge, F.B., J.G. Sampalo, J.L. Guedes and A.B. de Ulhoa, 1965. Biochemical studies on copper, copper oxidase, magnesium, sulfur, calcium and phosphorous in cancer of the breast. Clin. Chimica Acta, 12: 403-403.

Garg, A.N., S. Vivek, R.G. Weginwar and V.N., Sagdeo, 1994. An elemental correlation study in cancerous and normal breast tissue with successive clinical stages by neutron activation analysis. Biol. Trace Element Res., 46: 185-202.

Khan, P.K. and K.S. Awasthy, 2003. Cytogenetic toxicity of neem. Food Chem. Toxicol., 41: 1325-1328.
CrossRef  |  

Kusumran, W.R., A. Tepsuwan and P. Kuprodium, 1998. Antimutagenic and anticarcinogenic potential of some Thai vegetables. Mutation Res., 402: 247-258.

Lechaire, J.P., B. Shillito, G. Frebourg and F. Gaill, 2002. Elemental characterization of microorganism granules by EFTEM in the tube wall of a deep-sea vent invertebrate. Biol. Cell, 94: 243-249.
CrossRef  |  PubMed  |  Direct Link  |  

Mangal, P.C. and S. Kumar, 1984. Neutron activation analysis of trace elements in cancerous human breast tissue. Indian J. Physiol., 58: 355-355.

Mulay, I.L., R. Roy, B.E. Knox, N.H. Suhr and W.E. Delaney, 1971. Trace metal analysis by cancerous and non-cancerous human tissues. J. Nat. Cancer Inst., 47: 1-1.

Murthy, K.S., D.N. Rao, D.K. Rao and L.B.S. Murthy, 1978. A preliminary study on hypoglycemic and anti-hyperglycemic effects of Azadirachta indica. Indian J. Pharmacol., 10: 247-250.

Ng, K.H., D.A. Bradle, L.M. Looi, C.J. Mahmood and A.K. Wood, 1993. Differentiation of elemental composition of normal and malignant breast tissue by instrumental neutron activation analysis. Applied Radiation Isotope, 44: 511-511.

Patel, R.P. and B.M. Trivedi, 1962. In vitro bacterial activity of medicinal plants. Indian J. Med. Res., 5: 218-222.

Rizk, S.L. and H.H. Sky-Peck, 1984. Comparison between concentration of trace elements in normal and neoplastic human breast tissue. Cancer Res., 44: 5390-5390.

SaiRam, M., G. Ilavazhagan, S.K. Sharma, S.A. Dhanraj and B. Suresh et al., 2000. Anti-microbial activity of a new vaginal contraceptive NIM-76 from neem oil (Azadirachta indica). J. Ethnopharmacol., 71: 377-382.
CrossRef  |  Direct Link  |  

Schwartz, M.K., 1975. Role of trace elements in cancer. Cancer Res., 35: 3481-3481.

Solt, D. and E. Farber, 1976. New principle for the analysis of chemical carcinogenesis. Nature, 263: 701-703.
CrossRef  |  Direct Link  |  

Tang, Z.Y. and W. Shiu, 1994. Liver Cancer in Manual of Clinical Oncology. 6th Edn., Springer-Verlag, Berlin, pp: 303-309.

Udeinya, I.J., 1993. Antimalarial activity of Nigerian neem leaves. Trans. R. Soci. Trop. Med. Hyg., 87: 471-471.
Direct Link  |  

©  2019 Science Alert. All Rights Reserved
Fulltext PDF References Abstract