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The Effect of Neem (Azadirachta indica) Aqueous Extract and Dietary Selenium on Distribution of Selenium in Liver Tissue During Hepatocarcinogenesis



P. Hanachi , O. Fauziah , L.L. Nam , T.S. Tian , L.T. Peng , L.C. Weil and O.K. Ho
 
ABSTRACT

This study aimed to determine the distribution of selenium in the liver of rats during hepatocarcinogenesis when neem aqueous extract and dietary selenium was supplemented. The selenium distribution of the 9 diethylnitrosamine (DEN) and 2-acetylaminofluorene (2-AAF) induced hepatocarcinogenesis in Sprague dawley male rats as well as the other 9 normal rats, which were supplemented with 5% (w/v) neem leaves aqueous extract, 1.85 mg L-1 sodium selenate and tap water ad libitum according to the treatment groups for 10 weeks, were assessed with energy filter transmission electron microscope (EFTEM) by electron spectroscopic imaging (ESI)-3 window power law method. The result showed that the selenium distribution mean score in normal control group was the lowest and cancerous control group was the highest. However, the selenium distribution mean score in normal supplemented with 5% (w/v) neem leaves aqueous extract increased about 26% (p<0.05) compared to normal control group. This study suggested that neem leaves aqueous extract may be a potential primary chemopreventive agent rather than as secondary chemopreventive agent.

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  How to cite this article:

P. Hanachi , O. Fauziah , L.L. Nam , T.S. Tian , L.T. Peng , L.C. Weil and O.K. Ho , 2004. The Effect of Neem (Azadirachta indica) Aqueous Extract and Dietary Selenium on Distribution of Selenium in Liver Tissue During Hepatocarcinogenesis . Journal of Biological Sciences, 4: 584-587.

DOI: 10.3923/jbs.2004.584.587

URL: https://scialert.net/abstract/?doi=jbs.2004.584.587

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