Subscribe Now Subscribe Today
Research Article
 

The Effect of a Mannanoligosaccharides (Biomos®) on Necrotic Enteritis Infection in Broiler Chickens



Moemen A. Mohamed and Mohamed S. Abdel Hafez
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail
ABSTRACT

This study was conducted to determine the effect of Mannanoligosaccharide (Biomos®, Alltech Inc., IFT, Egypt) added to a broiler ration to reduce or control the prevalence of necrotic enteritis among broiler chicks reared under normal conditions. Seventy five, one-day-old, Cobb chicks were randomly divided to three groups of 25 birds for each. First group consists of nonchallenged negative control group, second group was a positive control group gavaged with C. perfringens (CP) (107 cfu/mL) daily for 3 consecutive days starting on d 16. The third group was supplemented with 2 g/kg ration from the start to the end of the experiment and orally challenged with C. perfringens type A at the the same method for positive control group. On days 1 (19-day-old) and 7 (25-day-old) post-challenge, lesion scores, mortalities and intestinal CP levels were assessed. The log10 C. perfringens/g of intestinal contents was significantly reduced in the biomos treated group (MTG) with a log10 value of 1.8 and 2.2 at the 1st and 7th days after challenge respectively compared with 4.3 and 4.7 at the same times respectively in the Positive Control Group (PC). Mortality was reduced to 8% in group supplemented with Biomos in contrast to 20% in positive control group. The present study revealed that the birds given biomos supplementation showed a reduction in the severity of lesion score which reached to 0.57 in contrast to 1.8 in positive control group. In summary, the findings of the present study strengthen the use of biomos as a safe alternative to antibiotics and attractive aid for prevention and control of Clostridium perferingens infections in broilers.

Services
Related Articles in ASCI
Similar Articles in this Journal
Search in Google Scholar
View Citation
Report Citation

 
  How to cite this article:

Moemen A. Mohamed and Mohamed S. Abdel Hafez, 2011. The Effect of a Mannanoligosaccharides (Biomos®) on Necrotic Enteritis Infection in Broiler Chickens. International Journal of Poultry Science, 10: 685-690.

DOI: 10.3923/ijps.2011.685.690

URL: https://scialert.net/abstract/?doi=ijps.2011.685.690

REFERENCES
1:  Abbas, A.K., A.H. Litcman and J.S. Pober, 2000. Cellular and Molecular Immunology. 4th Edn., Saunders, Philadelphia, USA., Pages: 553.

2:  Annett, C.B., J.R. Viste, M.C. Trejo, H.L. Classen, D.M. Midddleton and E. Simko, 2002. Necrotic enteritis: Effects of barley, wheat and corn diets on proliferation of Clostridium perfringens type A. Avian. Pathol., 31: 599-602.

3:  Biggs, P., C.M. Parsons and G.C. Fahey, 2007. The effects of several oligosaccharides on growth performance, nutrient digestibilities and cecal microbial populations in young chicks. Poult. Sci., 86: 2327-2336.
CrossRef  |  Direct Link  |  

4:  Bornet, F.R., 1997. Bio-Mos Poultry Dossier. Alltech Inc., Nicholasville, USA..

5:  Bornet, F.R.J., F. Brouns, Y. Tashiro and V. Duvillier, 2002. Nutritional aspects of short-chain fructooligosaccharides: Natural occurrence, chemistry, physiology and health implications. Dig. Liver Dis., 34: S111-S120.
CrossRef  |  Direct Link  |  

6:  Brouns, F., B. Kettlitz and E. Arrigoni, 2002. Resistant starch and the butyrate revolution. Trends Food Sci. Technol., 3: 251-261.
CrossRef  |  Direct Link  |  

7:  Delzenne, N.M., 2003. Oligosaccharides: State of the art. Proc. Nutr. Soc., 62: 177-182.

8:  Diarra, M.S., F.G. Silversides, F. Diarrasouba, J. Pritchard and L. Masson et al., 2007. Impact of feed supplementation with antimicrobial agents on growth performance of broiler chickens, Clostridium perfringens and Enterococcus counts and antibiotic resistance phenotypes and distribution of antimicrobial resistance determinants in Escherichia coli isolates. Applied Environ. Microbiol., 73: 6566-6576.
CrossRef  |  

9:  Fernandez, F., M. Hinton and B. van Gils, 2002. Dietary mannan-oligosaccharides and their effect on chicken caecal microflora in relation to Salmonella Enteritidis colonization. Avian Pathol., 31: 49-58.
CrossRef  |  Direct Link  |  

10:  Ferket, P.R., C.W. Parks and J.L. Grimes, 2002. Benefits of dietary antibiotics and mannanoligosaccharide supplementation for poultry. Proceedings of the Multi-State Poultry Meeting, May 14-16, 2002, Atlanta, GA., USA -.

11:  Fukata, T., Y. Hadate, E. Baba and A. Arakawa, 1991. Influence of bacteria on Clostridium perfringens infections in young chickens. Avian Dis., 35: 224-227.

12:  Gao, J., H.J. Zhang, S.H. Yu, S.G. Wu and I. Yoon et al., 2008. Effects of yeast culture in broiler diets on performance and immunomodulatory functions. Poult. Sci., 87: 1377-1384.
CrossRef  |  PubMed  |  Direct Link  |  

13:  Heier, B.T., A. Lovland, K.B. Soleim, M. Kaldhusdal and J. Jarp, 2001. A field study of naturally occurring specific antibodies against Clostridium perfringens alpha-toxin in Norwegian broiler flocks. Avian Dis., 45: 724-732.
PubMed  |  

14:  Kaldhusdal, M. and A. Lovland, 2000. The economic importance of Clostridium perfringens is greater than anticipated. World's Poult. Sci., 16: 50-51.

15:  Kulkarni, R.R., V.R. Parreira, Y.F. Jiang and J.F. Prescott, 2010. Alive oral recombinant Salmonella enteric serovar Typhimurium vaccine expressing Clostridium perfringens antigens confers protection against necrotic enteritis in broiler chickens. Clin. Vaccine Immunol., 17: 205-214.

16:  Lee, D.J., R.A. Drongowski, A.G. Coran and C.M. Harmon, 2000. Evaluation of probiotic treatment in a neonatal animal model. Pediatr. Surg. Int., 16: 237-242.
PubMed  |  

17:  Line, E.J., S.J. Bailey, N.A. Cox, N.J. Stern and T. Tompkins, 1998. Effect of yeast-supplemented feed on Salmonella and Campylobacter populations in broilers. Poult. Sci., 77: 405-410.
CrossRef  |  PubMed  |  Direct Link  |  

18:  Loddi, M.M., I.S.O. Nakaghi, F. Edens, F.M. Tucci, M.I. Hannas, V.M. Moraes and J.A. Ariki, 2002. Effect of mannanoligosaccharide and organic acids on intestinal morphology integrity of broiler evaluated by scanning electron microscope. Proceedings of the 11th European Poultry Science Conference, September 6-10, 2002, Bremen, Germany, pp: 121-.

19:  Lovland, A. and M. Kaldhusdal, 1999. Liver lesions seen at slaughter as an indicator of necrotic enteritis in broiler flocks. FEMS Immunol. Med. Micbiol., 24: 345-351.

20:  McDevitt, R.M., J.D. Brooker, T. Acamovic and N.H.C. Sparks, 2006. Necrotic enteritis; a continuing challenge for the poultry industry. World's Poult. Sci. J., 62: 221-247.
CrossRef  |  Direct Link  |  

21:  McReynolds, J., C. Waneck, J. Byrd, K. Genovese, S. Duke and D. Nisbet, 2009. Efficacy of multistrain direct-fed microbial and phytogenetic products in reducing necrotic enteritis in commercial broilers. Poult. Sci., 88: 2075-2080.
Direct Link  |  

22:  Mohamed, M.A., O.A. Sylvia and T.Y. Abdel Motelib, 2009. Associated profiles of virulence gene markers in Clostridium perfringens strains isolated from healthy and diseased broiler chickens with necrotic enteritis. Assiut Vet. Med. J., 55: 255-275.

23:  Nisbet, D.J., D.E. Corrier, S.C. Ricke, M.E. Hume, J.A. Byrd and J.R. DeLoach, 1996. Cecal propionic acid as a biological indicator of the early establishment of a microbial ecosystem inhibitory to Salmonella in chicks. Anaerobe, 2: 345-350.

24:  Peuranen, S., A. Kocher and K.A. Dawson, 2006. Yeast cell preparations prevent the attachment of enteropathogenic Escherichia coli on broiler gut mucus. Reprod. Nutr. Dev., 46: S111-S113.

25:  Prescott, J.F., R. Sivendra and D.A. Barnum, 1978. The use of bacitracin in the prevention and treatment of experimentally-induced necrotic enteritis in the chicken. Can. Vet. J., 19: 181-183.
PubMed  |  Direct Link  |  

26:  Tech, R., 1999. Necrotic enteritis and associated conditions in broiler chicken. World Poult., 15: 44-47.

27:  Williams, R.B., 2002. Anticoccidial vaccines for broiler chickens: Pathways to success. Avian Pathol., 31: 317-353.

©  2021 Science Alert. All Rights Reserved