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Research Journal of Mutagenesis
  Year: 2014 | Volume: 4 | Issue: 1 | Page No.: 14-22
DOI: 10.3923/rjmutag.2014.14.22
 
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Germination Response of Five Rice Varieties Treated with Sodium Azide

Ujomonigho E. Omoregie, Joseph K. Mensah and Beckley Ikhajiagbe

Abstract:
The present study investigated the effects of sodium azide pretreatment of grains on the germination of five varieties of rice. Germination parameters of FARO 44, FARO 52, FARO 57, NERICA L-34 and NERICA L-47 rice varieties treated with different concentrations of sodium azide was studied in petri dish experiments. Lethal dose (LD50) was estimated at 0.032% sodium azide. The concentrations of sodium azide solution (0.004, 0.008, 0.016 and 0.032% sodium azide at pH 3) were prepared on volume/weight basis. Control was distilled water at pH 7. Viable grains were pretreated in sodium azide solutions for 6 h. Germination was monitored from the second to fourteenth Day after Initiation (DAI). There were significant differences (p = 0.05) in percentage germination, length of radicles, length of plumules and dry weights of sprouting grains. Germination percentage on the fourth day after initiation was 0% in FARO 57 and NERICA L-47 pre-treated in 0.032% sodium azide. At 14 DAI, germination percentage in all varieties including the control ranged between 94 and 100%. The longest and shortest radicles were both obtained in NERICA L-47 (86.25, 2.50 mm) in 0.032 and 0%, respectively. The shortest plumules (1.00 mm) were obtained in both NERICA L-34 and NERICA L-47, pre-treated in 0.032% sodium azide with and longest (50.75 mm) in 0% sodium azide pre-treated NERICA L-47 at 6 DAI. The experiment showed that sodium azide significantly delayed but did not inhibit germination.
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How to cite this article:

Ujomonigho E. Omoregie, Joseph K. Mensah and Beckley Ikhajiagbe, 2014. Germination Response of Five Rice Varieties Treated with Sodium Azide. Research Journal of Mutagenesis, 4: 14-22.

DOI: 10.3923/rjmutag.2014.14.22

URL: https://scialert.net/abstract/?doi=rjmutag.2014.14.22

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