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Research Journal of Mutagenesis

Year: 2014 | Volume: 4 | Issue: 1 | Page No.: 14-22
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Authors

Ujomonigho E. Omoregie

Joseph K. Mensah

Beckley Ikhajiagbe

Keywords

The present study investigated the effects of sodium azide pretreatment of grains on the germination of five varieties of rice. Germination parameters of FARO 44, FARO 52, FARO 57, NERICA L-34 and NERICA L-47 rice varieties treated with different concentrations of sodium azide was studied in petri dish experiments. Lethal dose (LD50) was estimated at 0.032% sodium azide. The concentrations of sodium azide solution (0.004, 0.008, 0.016 and 0.032% sodium azide at pH 3) were prepared on volume/weight basis. Control was distilled water at pH 7. Viable grains were pretreated in sodium azide solutions for 6 h. Germination was monitored from the second to fourteenth Day after Initiation (DAI). There were significant differences (p = 0.05) in percentage germination, length of radicles, length of plumules and dry weights of sprouting grains. Germination percentage on the fourth day after initiation was 0% in FARO 57 and NERICA L-47 pre-treated in 0.032% sodium azide. At 14 DAI, germination percentage in all varieties including the control ranged between 94 and 100%. The longest and shortest radicles were both obtained in NERICA L-47 (86.25, 2.50 mm) in 0.032 and 0%, respectively. The shortest plumules (1.00 mm) were obtained in both NERICA L-34 and NERICA L-47, pre-treated in 0.032% sodium azide with and longest (50.75 mm) in 0% sodium azide pre-treated NERICA L-47 at 6 DAI. The experiment showed that sodium azide significantly delayed but did not inhibit germination.
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