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Research Journal of Mutagenesis

Year: 2014 | Volume: 4 | Issue: 1 | Page No.: 14-22
Research Article

Germination Response of Five Rice Varieties Treated with Sodium Azide

Ujomonigho E. Omoregie
Department of Plant Biology and Biotechnology, University of Benin, Nigeria
Joseph K. Mensah
Department of Botany, Ambrose Alli University, Ekpoma, Nigeria
Beckley Ikhajiagbe
Department of Plant Biology and Biotechnology, University of Benin, Nigeria
The present study investigated the effects of sodium azide pretreatment of grains on the germination of five varieties of rice. Germination parameters of FARO 44, FARO 52, FARO 57, NERICA L-34 and NERICA L-47 rice varieties treated with different concentrations of sodium azide was studied in petri dish experiments. Lethal dose (LD50) was estimated at 0.032% sodium azide. The concentrations of sodium azide solution (0.004, 0.008, 0.016 and 0.032% sodium azide at pH 3) were prepared on volume/weight basis. Control was distilled water at pH 7. Viable grains were pretreated in sodium azide solutions for 6 h. Germination was monitored from the second to fourteenth Day after Initiation (DAI). There were significant differences (p = 0.05) in percentage germination, length of radicles, length of plumules and dry weights of sprouting grains. Germination percentage on the fourth day after initiation was 0% in FARO 57 and NERICA L-47 pre-treated in 0.032% sodium azide. At 14 DAI, germination percentage in all varieties including the control ranged between 94 and 100%. The longest and shortest radicles were both obtained in NERICA L-47 (86.25, 2.50 mm) in 0.032 and 0%, respectively. The shortest plumules (1.00 mm) were obtained in both NERICA L-34 and NERICA L-47, pre-treated in 0.032% sodium azide with and longest (50.75 mm) in 0% sodium azide pre-treated NERICA L-47 at 6 DAI. The experiment showed that sodium azide significantly delayed but did not inhibit germination.
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References

  1. Ahloowalia, B.S. and M. Maluszynski, 2001. Induced mutations-A new paradigm in plant breeding. Euphytica, 118: 167-173.
    CrossRefDirect Link
  2. Akinwale, M.G., B.O. Akinyele, A.C. Odiyi, F. Nwilene, G. Gregorio and O.E. Oyetunji, 2012. Phenotypic screening of nigerian rain-fed lowland mega rice varieties for submergence tolerance. Proceedings of the World Congress on Engineering, July 4-6, 2012, London, pp: 640.
  3. Alika, J.E., 2006. Statistics and Research Methods. 2nd Edn., AMBIK Press, Benin City, Pages: 366.
  4. Al-Qurainy, F. and S. Khan, 2009. Mutagenic effects of sodium azide and its application in crop improvement. World Applied Sci. J., 6: 1589-1601.
    Direct Link
  5. Bradford, K.J., 1990. A water relations analysis of seed germination rates. Plant Physiol., 94: 840-849.
    CrossRef
  6. Cheng, X. and M. Gao, 1988. Biological and genetic effects of combined treatments of sodium azide, gamma rays and EMS in barley. Environ. Exp. Bot., 28: 281-288.
    CrossRef
  7. Crawford, G.W. and C. Shen, 1998. The origins of rice agriculture: Recent progress in East Asia. Antiquity, 72: 858-866.
    CrossRefDirect Link
  8. Gichner, T. and J. Veleminsky, 1977. The very low mutagenic activity of sodium azide in Arabidopsis thaliana. Biol. Plant., 19: 153-155.
    CrossRefDirect Link
  9. Kamra, O.P. and B. Gollapudi, 1979. Mutagenic effects of sodium azide in Drosophila melanogaster. Mutation Res./Genet. Toxicol., 25: 381-384.
    CrossRefPubMedDirect Link
  10. Khan, M.A. and S. Gulzar, 2003. Germination responses of Sporobolus ioclados: A saline desert grass. J. Arid Environ., 53: 387-394.
    CrossRefDirect Link
  11. Khan, S., M.R. Wani and K. Parveen, 2004. Induced genetic variability for quantitative traits in Vigna radiate (L.) Wilczek. Pak. J. Bot., 36: 845-850.
    Direct Link
  12. Khan, S., M.R. Wani and K. Parveen, 2005. Chlorophyll mutations induced in mungbean by chemical mutagens. Adv. Plant Sci., 18: 343-347.
  13. Kiruki, S., L.A. Onek and M. Limo, 2006. Azide-based mutagenesis suppresses Striga hermonthica seed germination and parasitism on maize varieties. Afr. J. Biotechnol., 5: 866-870.
  14. Kleinhofs, A., C. Sander, R.A. Nilian and C.F. Konzak, 1974. Azide Mutagenicity-Mechanism and Nature of Mutants Produced. In: Polyploidy and Induced Mutations in Plant Breeding: Proceedings of a Meeting on Mutation and Polyploidy and of a Research Co-Ordination Meeting on Improvement of Mutation Breeding Techniques, Held in Bari, October 2-10,1972, FAO and IAEA (Eds.). IAEA, Vienna, pp: 195-199.
  15. Kleinhofs, A., W.M. Owais and R.A. Nilan, 1978. Azide. Mutat. Res./Rev. Genet. Toxicol., 55: 165-195.
    CrossRefDirect Link
  16. Lal, G.M., B. Toms and S. Smith, 2009. Induced chlorophyll mutations in black gram. Asian J. Agric. Sci., 1: 1-3.
    Direct Link
  17. Maji, A.T., 2009. He knows what he wants. Proceedings of the Rice to Feed Africa: Stories from the Emergency Rice Initiative Project Workshop, November 2009, Benin, pp: 5.
  18. Mensah, J.K. and B. Obadoni, 2007. Effects of sodium azide on yield parameters of groundnut (Arachis hypogaea L.). Afr. J. Biotechnol., 6: 668-671.
    Direct Link
  19. Mostafa, G.G., 2011. Effect of sodium azide on the growth and variability induction in Helianthus annuus L. Int. J. Plant Breed. Genet., 5: 76-85.
    CrossRefDirect Link
  20. Niknejad, M., 1975. The Effect of Duration and Condition of Post-Treatment Storage on the Physiological Damage and Mutagenic Frequency of Barley Treated with Different Concentrations of Sodium Azide. In: Barley Genetics III, Gaul, H. (Ed.). Verlag Karl Thiemig, Munich, Germany, ISBN-13: 9783521061088, pp: 132-145.
  21. Owais, W.M. and A. Kleinhofs, 1988. Metabolic activation of the mutagen azide in biological systems. Fundam. Mol. Mechanisms Mutagenesis, 197: 313-323.
    CrossRefPubMedDirect Link
  22. Pearson, O.W., R.A. Nilan and C. Sander, 1974. The effect of sodium azide on the cell cycle of the embryonic barley shoot. Barley Genet. Newslett., 4: 61-61.
  23. Pearson, O.W., C. Sander and R.A. Nilan, 1975. The effect of sodium azide on cell processes in the embryonic barley shoot. Radiation Bot., 15: 315-322.
    CrossRefDirect Link
  24. Singh, V.P. and R.D.S. Yadav, 1987. Induced mutations for quantitative and qualitative traits in green gram and lentil. J. Genet. Plant Breed., 45: 1-5.
  25. Sinha, A. and J.P. Lal, 2007. Effect of mutagens on M1 parameters and qualitative changes induced in M2 generation in lentil. Legume Res. Int. J., 30: 180-185.
    Direct Link
  26. Suzuki, Y., Y. Sano, K. Ise, U. Matsukura, N. Aoki and H. Sato, 2008. A rice mutant with enhanced amylose content in endosperm without affecting amylopectin structure. Breed. Sci., 58: 209-215.
    CrossRef
  27. Van der Veen, J.H., 1966. Sterility, embryonic lethals and chlorophyll mutations. Arabidopsis Inform. Service, 33: 26-26.
    Direct Link
  28. Yuan, H.Y. and Z.L. Zhang, 1993. Effect of free radicals and temperature on sister chromatid exchange in Hordeum vulgare L. Acta Bot. Sin., 35: 20-26.
    Direct Link

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