Subscribe Now Subscribe Today
Science Alert
 
Blue
   
Curve Top
International Journal of Pharmacology
  Year: 2019 | Volume: 15 | Issue: 2 | Page No.: 265-273
DOI: 10.3923/ijp.2019.265.273
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Hepatic Gene Expression, Antioxidant Enzymes and Anti-diabetic Effect of Nigella sativa in Diabetic Rats
Thnaian Althnaian, Ibrahim Albokhadaim and Sabry M. El-Bahr

Abstract: Background and Objectives: Role of black cumin seeds (Nigella sativa) in enhancing gene expressions of catalase (CAT), glutathione-s-transferase (GST) and B-cell CLL/lymphoma 2 (Bcl-2) in diabetic rats has not investigated so far. Furthermore, sensitive technique is required to confirm its overexpression to superoxide dismutase (SOD), glutathione peroxidase (GPX) and insulin like growth factor-1 (IGF-1). This study was aimed to investigate the effect of black seed on gene expression of CAT, GST and Bcl-2 and also examine its expression to SOD, GPX and IGF-1 by high sensitive RT-PCR in diabetic rats. Materials and Methods: Eight non-diabetic albino rats assigned as control (group I). Sixteen rats rendered diabetic by streptozotocin (STZ; 60 mg kg–1 b.wt.). Diabetic rats divided into group II and group III (eight rats in each). Rats in group II were untreated diabetic group while rats of group III were diabetic and treated with Black-cumin seed (2 g kg–1 b.wt.) for 6 weeks. Results: Diabetes increased blood glucose level than control while black seed reduced the higher blood glucose in diabetic rats significantly. Except for IGF-1, diabetes induced significant increases in gene expressions of Bcl2, CAT, SOD, GPX and GST compare to control. Black seed increased expression of Bcl2, CAT, SOD, GPX, GST and IGF-1 genes compare to both control and diabetic untreated rats. Conclusion: The current study observed that the Nigella sativa showed overexpression to CAT, GST and Bcl2 genes and also to SOD, GPX and IGF-1 by higher quantitative PCR in STZ diabetic rats.
PDF Fulltext XML References Citation Report Citation
 RELATED ARTICLES:
  •    Effect of Aqueous Extract of Fenugreek (Trigonella foenum-graecum L.) On Selected Biochemical and Oxidative Stress Biomarkers in Rats Intoxicated with Carbon Tetrachloride
  •    Effect of N. sativa Oil on Impaired Glucose Tolerance and Insulin Insensitivity Induced by High-Fat-Diet and Turpentine-Induced Trauma
  •    Effect of Aflatoxin B1 on Histopathology and Oxidative Stress Biomarkers in Testis of Rats with Special References to Gene Expression of Antioxidant Enzymes
How to cite this article:

Thnaian Althnaian, Ibrahim Albokhadaim and Sabry M. El-Bahr, 2019. Hepatic Gene Expression, Antioxidant Enzymes and Anti-diabetic Effect of Nigella sativa in Diabetic Rats. International Journal of Pharmacology, 15: 265-273.

DOI: 10.3923/ijp.2019.265.273

URL: https://scialert.net/abstract/?doi=ijp.2019.265.273
COMMENT ON THIS PAPER
 
 
 

 
  Bullet Navigation
 
  Page Icon Online First
  Page Icon Current Issue
  Page Icon Previous Issues
  Page Icon Editorial Board
  Page Icon Submit a Manuscript
  Page Icon Guide to Authors
  Page Icon Subscribe to E-alerts

  Bullet Indexed In
 
  Page Icon ASCI-Database
  Page Icon Asian Digital Library
  Page Icon Chemical Abstract Services
  Page Icon EMBASE
  Page Icon Google Scholar
  Page Icon ISI Web of Knowledge
  Page Icon Science Citation Index (EXP)
  Page Icon SCIMAGO
  Page Icon Thomson ISI

Science Alert
Curve Bottom