Camel Milk Regulates Gene Expression and Activities of Hepatic Antioxidant Enzymes in Rats Intoxicated with Carbon Tetrachloride

Research Article

Camel Milk Regulates Gene Expression and Activities of Hepatic Antioxidant Enzymes in Rats Intoxicated with Carbon Tetrachloride

The objective of the present study was to investigate the effect of camel milk on activities and gene expression of hepatic antioxidant enzymes in rats intoxicated with carbon tetrachloride (CCl₄). Therefore, twenty four rats were used in the current study. Rats were divided into four groups, the first and second groups were received water and camel's milk, respectively whereas rats of both third and fourth groups were injected with CCl₄ and received water and camel's milk, respectively. After 5 weeks, liver tissues were collected for biochemical analysis of the activities and gene expression of antioxidant enzymes. Rats supplemented with camel milk alone showed no significant difference in all examined parameters compared to control rats. Liver damage and oxidative stress were evident in untreated CCl₄ intoxicated rats as indicated by significant elevation of hepatic enzymes, significant elevation in thiobarbituric acid reactive substance (TBARS), significant reduction in reduced glutathione level (GSH), significant reduction in the activities of catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPX) and glutathione-S transferase (GST) and finally significant down-regulation of antioxidant enzymes gene expression compare to control. Administration of camel milk along with CCl₄ caused amelioration in CCl₄-induced effects compare to the untreated CCl₄ intoxicated rats via up-regulation of antioxidant enzyme gene expression, activation of the expressed genes and increasing the availability of GSH. Conclusively, camel milk exerted its protective effect against CCl₄ induced hepatic toxicity by modulating the extent of lipid peroxidation and augmenting antioxidant defense system at activity and gene expression levels.

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S.M. El-Bahr, 2014. Camel Milk Regulates Gene Expression and Activities of Hepatic Antioxidant Enzymes in Rats Intoxicated with Carbon Tetrachloride. Asian Journal of Biochemistry, 9: 30-40.

DOI: 10.3923/ajb.2014.30.40

URL: https://scialert.net/abstract/?doi=ajb.2014.30.40

Asian Journal of Biochemistry

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S.M. El-Bahr

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Research Article

Camel Milk Regulates Gene Expression and Activities of Hepatic Antioxidant Enzymes in Rats Intoxicated with Carbon Tetrachloride

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