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Trends in Bioinformatics
  Year: 2013 | Volume: 6 | Issue: 2 | Page No.: 45-61
DOI: 10.3923/tb.2013.45.61
 
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In silico Structural Analysis of 16S rDNA Sequences of Bacteria Isolated from Keratitis Patients
Anusha Bhaskar and V. Nithya

Abstract:
Keratitis is a condition in which the cornea of the eye becomes inflamed. It is caused by bacteria, viruses, fungi and parasites. The diagnosis of the causative organism of keratitis remains a problem due to lack of advanced techniques. In this study PCR and sequence analysis of 16S rDNA was used to ascertain the bacteria isolated in eye swabs of keratitis patients. The bacterial genes were sequenced and deposited in the GenBank (NCBI) with the accession numbers JN378393, JN378392, HM204502, JQ039348, JN652127, JN652129, JQ039350, JN652128, JQ039349 and HQ404365 for Streptococcus viridan, Staphylococcus aureus, Micrococcus sp., Moraxella sp., Citrobacter koseri, Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella sp., Propionibacterium sp. and Staphylococcus epidermidis, respectively. Sequences were analyzed and aligned by ClustalX and a phylogenetic relationship was studied. The secondary structure of the 16S DNA was developed using GeneBee which produces secondary structures prediction through the minimization of the potential energy of the system. These results may be useful in characterizing the micro evolutionary mechanisms of the species for researchers.
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How to cite this article:

Anusha Bhaskar and V. Nithya, 2013. In silico Structural Analysis of 16S rDNA Sequences of Bacteria Isolated from Keratitis Patients. Trends in Bioinformatics, 6: 45-61.

DOI: 10.3923/tb.2013.45.61

URL: https://scialert.net/abstract/?doi=tb.2013.45.61

 
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