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Research Journal of Phytochemistry
  Year: 2014 | Volume: 8 | Issue: 4 | Page No.: 199-204
 
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Preliminarily Investigation on Antioxidant Phytochemical in Some Medicinal Plants of Kumaon Region

Kundan Prasad, Kalpana Moulekhi and G. Bisht

Abstract:
While screening Asparagus adscendens Roxb, Vitex negundo Linn. and Stephania glabra Miers in search of natural antioxidant, we first time developed HPLC method for the determination of α-carotene, β-carotene, xanthophyll and DL-α-tocopherol in the said spices. These antioxidants were extracted in light petroleum ether/methanol/ethyl acetate (1:1:1 ratio) and separated by phase separation method. Chromatogram of carotenoids and DL-α-tocopherol showed characteristic absorbance at 450 and 291 nm, respectively. This method was successfully applied to the analysis of antioxidants of these plants. The retention time of xanthophyll, α-carotene and β-carotene were found 2.045, 10.947 and 11.495 min, respectively. The retention time of DL-α-tocopherol was found 11.780 min. The maximum content of xanhophyll (8.08±0.93 mg/100 g), α-carotene (3.89±0.53 mg/100 g), β-carotene (243.53±0.66 mg/100 g) and total phenolics (324.55±0.47 mg/100 g) was found in V. negundo under the three investigated plants. The content of α-tocopherol (11.32±0.19 mg/100 g) and vitamin-c (311.45±0.47 mg/100 g) were maximum in A. adscendens. In the present study, the plants are found good source of antioxidants.
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How to cite this article:

Kundan Prasad, Kalpana Moulekhi and G. Bisht, 2014. Preliminarily Investigation on Antioxidant Phytochemical in Some Medicinal Plants of Kumaon Region. Research Journal of Phytochemistry, 8: 199-204.

URL: https://scialert.net/abstract/?doi=rjphyto.2014.199.204

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