Research Journal of Phytochemistry1819-34712151-6081Science International10.3923/rjphyto.2014.199.204PrasadKundan MoulekhiKalpana BishtG. 4201484While screening Asparagus adscendens Roxb, Vitex negundo Linn. and Stephania glabra Miers in search of natural antioxidant, we first time developed HPLC method for the determination of α-carotene, β-carotene, xanthophyll and DL-α-tocopherol in the said spices. These antioxidants were extracted in light petroleum ether/methanol/ethyl acetate (1:1:1 ratio) and separated by phase separation method. Chromatogram of carotenoids and DL-α-tocopherol showed characteristic absorbance at 450 and 291 nm, respectively. This method was successfully applied to the analysis of antioxidants of these plants. The retention time of xanthophyll, α-carotene and β-carotene were found 2.045, 10.947 and 11.495 min, respectively. The retention time of DL-α-tocopherol was found 11.780 min. The maximum content of xanhophyll (8.08±0.93 mg/100 g), α-carotene (3.89±0.53 mg/100 g), β-carotene (243.53±0.66 mg/100 g) and total phenolics (324.55±0.47 mg/100 g) was found in V. negundo under the three investigated plants. The content of α-tocopherol (11.32±0.19 mg/100 g) and vitamin-c (311.45±0.47 mg/100 g) were maximum in A. adscendens. In the present study, the plants are found good source of antioxidants.]]>Anonymous,1976Vol. 10,Pages: 577Pages: 577Beecher, G.R. and F. Khachik,19847313971404Bernstein, P.S., F. Khachik, L.S. Carvalho, G.J. Muir, D.Y. Zhao and N.B. Katz,200172215223Chopra, R.N., S.L. Nayar and I.C. Chopra,1956pp: 256-257pp: 256-257Javanmardi, J., A. Khalighi, A. Kashi, H.P. Bais and J.M. Vivanco,2002Ocimum basilicum L.) found in local accessions and used in traditional medicines in Iran.]]>5058785883Kahkonen, M.P., A.I. Hopia, H.J. Vuorela, J.P. Rauha, K. Pihlaja, T.S. Kujala and M. Heinonen,19994739543962Kurilich, A.C., G.J. Tsau, A. Brown, L. Howard and B.P. Klein et al.,1999Brassica oleracea.]]>4715761581Landrum, J.T., R.A. Bone, H. Joa, M.D. Kilburn, L.L. Moore and K.E. Sprague,1997655762Larson, R.A.,198827969978Mazumder, A., N. Neamati, S. Sunder, J. Schulz, H. Pertz, E. Eich and Y. Pommier,19974030573063Osmaston, A.E.,1976pp: 540-541pp: 540-541Prasad, S. and S.P. Wahi,1965Vitex negundo Linn. (Nirgundi).]]>72208211Ranganna, S.,19762nd Edn.,Pages: 545Pages: 545Singleton, V.L., R. Orthofer and R.M. Lamuela-Raventos,19991999pp: 152-178pp: 152-178Skibsted, L.H., U.C. Charlotte, V.K.O. Maiken, V.H. Rikke, B.M. Bibby and K. Dorthe,2005604448Sun, Y.,19908583599