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Research Journal of Immunology
  Year: 2012 | Volume: 5 | Issue: 1 | Page No.: 1-16
DOI: 10.3923/rji.2012.1.16
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Development of a Simple Method for a New Immunoconjugate Utilizing Laccase

S. Ray, N. Das and B. Bishayi

Enzyme immunoassays utilize immunoglobulins conjugated to different enzyme labels or marker enzymes. In the current study, an immunoconjugate is being developed utilising the fungal enzyme laccase. The efficacy of this newly developed immunolaccase conjugate, prepared by standardized conjugation methods (glutaraldehyde and periodate based), has been demonstrated in indirect enzyme-immunoassay, for detection of purified rabbit IgG. The results of the assay indicated this newly developed immunolaccase conjugate to be potent for the detection of purified rabbit IgG when compared with commercially available peroxidase conjugated immunoglobulin or immunoperoxidase conjugate.
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  •    Isolation, Culture Optimization and Physico-chemical Characterization of Laccase Enzyme from Pleurotus fossulatus
  •    Comparative Immune Response Pattern of Commercial Infectious Bursal Disease Vaccines Against Field Isolates in Pakistan
  •    Experimental Evidence for Presence of a Growth Regulating Extracellular Laccase in Some Pleurotus Species
  •    Microbial Laccases and their Applications: A Review
  •    Lumpy Skin Disease Virus Identification in Different Tissues of Naturally Infected Cattle and Chorioallantoic Membrane of Emberyonated Chicken Eggs Using Immunofluorescence, Immunoperoxidase Techniques and Polymerase Chain Reaction
  •    Prevalence of Infectious Bovine Rhinotracheitis and Bovine Viral Diarrhoea Viruses in Female Buffaloes with Reproductive Disorders and Parasitic Infections
How to cite this article:

S. Ray, N. Das and B. Bishayi, 2012. Development of a Simple Method for a New Immunoconjugate Utilizing Laccase. Research Journal of Immunology, 5: 1-16.

DOI: 10.3923/rji.2012.1.16






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