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Pakistan Journal of Biological Sciences
  Year: 2014 | Volume: 17 | Issue: 4 | Page No.: 529-534
DOI: 10.3923/pjbs.2014.529.534
 
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Production, Optimization and Characterization of Fibrinolytic Enzyme by Bacillus subtilis RJAS19

D.J. Mukesh Kumar, R. Rakshitha, M. Annu Vidhya, P. Sharon Jennifer, Sandip Prasad, M. Ravi Kumar and P.T. Kalaichelvan

Abstract:
The present study aimed at the production, purification and characterization of fibrinolytic nattokinase enzyme from the bacteria isolated from natto food. For the purpose, a fibrinolytic bacterium was isolated and identified as Bacillus subtilis based on 16S rDNA sequence analysis. The strain was employed for the production and optimization of fibrinolytic enzyme. The strain showed better enzyme production during 72nd h of incubation time with 50°C at the pH 9. The lactose and peptone were found to be increasing the enzyme production rate. The enzyme produced was purified and also characterized with the help of SDS-PAGE analysis. The activity and stability profile of the purified enzyme was tested against different temperature and pH. The observations suggesting that the potential of fibrinolytic enzyme produced by Bacillus subtilis RJAS19 for its applications in preventive medicines.
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How to cite this article:

D.J. Mukesh Kumar, R. Rakshitha, M. Annu Vidhya, P. Sharon Jennifer, Sandip Prasad, M. Ravi Kumar and P.T. Kalaichelvan, 2014. Production, Optimization and Characterization of Fibrinolytic Enzyme by Bacillus subtilis RJAS19. Pakistan Journal of Biological Sciences, 17: 529-534.

DOI: 10.3923/pjbs.2014.529.534

URL: https://scialert.net/abstract/?doi=pjbs.2014.529.534

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