Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2014.529.534Bacillus subtilis RJAS19]]>KumarD.J. Mukesh RakshithaR. VidhyaM. Annu JenniferP. Sharon PrasadSandip KumarM. Ravi KalaichelvanP.T. 42014174The present study aimed at the production, purification and characterization of fibrinolytic nattokinase enzyme from the bacteria isolated from natto food. For the purpose, a fibrinolytic bacterium was isolated and identified as Bacillus subtilis based on 16S rDNA sequence analysis. The strain was employed for the production and optimization of fibrinolytic enzyme. The strain showed better enzyme production during 72nd h of incubation time with 50°C at the pH 9. The lactose and peptone were found to be increasing the enzyme production rate. The enzyme produced was purified and also characterized with the help of SDS-PAGE analysis. The activity and stability profile of the purified enzyme was tested against different temperature and pH. The observations suggesting that the potential of fibrinolytic enzyme produced by Bacillus subtilis RJAS19 for its applications in preventive medicines.]]>Al-Juamily, E.F. and B.H. Al-Zaidy,2012Bacillus lichniformis B4 local isolate.]]>3289295Bradford, M.M.,197672248254Chang, C.T., M.H. Fan, F.C. Kuo and H.Y. Sung,2000Bacillus subtilis IMR-NK1.]]>4832103216Borah, D., R. Yadav, A. Sangra, L. Shahin and A.K. Chaubey,2012Bacillus subtilis, isolated from tea garden soil samples of Dibrugarh, Assam.]]>5124125Dubey, R., J. Kumar, D. Agrawala, T. Char and P. Pusp,20111014081420Fujita, M., Y. Ito, K. Hong and S. Nishimuro,19959157164Gupta, R., Q. Beg and P. Lorenz,2002591532Henriette, C., S. Zinebi, M.F. Aumaitre, E. Petitdemange and H. Petitdemange,1993Serratia marcescens.]]>12129135Kim, W., K. Choi, Y. Kim, H. Park and J. Choi et al.,1996Bacillus sp. strain CK 11-4 screened from Chungkook-Jang.]]>6224822488Kiran, G.S., S. Shanmughapriya, S. Jayalakshmi, J. Selvin and R. Gandhimathi et al.,2008Pseudomonas sp. (MSI057).]]>31483492Krishnaveni, A. and T.S. Rani,2011Argyreia nervosa.]]>22831Laemmli, U.K.,1970227680685Yin, L.J., H.H. Lin and S.T. Jiang,2010Bacillus subtilis YJ1.]]>5857375742Milner, M. and K. Makise,20028157164Peng, Y., Q. Huang, R.H. Zhang and Y.Z. Zhang,2003Bacillus amyloliquefaciens DC-4 screened from dou-chi, a traditional Chinese soybean food.]]>1344552Negi, S. and R. Banerjee,2010Aspergillus awamori in a single fermentation.]]>47380Saurabh, S., I. Jasmine, G. Pritesh and S.R. Kumar,2007Bacillus sp. using soybean as substrate.]]>316Sumi, H., H. Hamada, H. Tsushima, H. Mihara and H. Muraki,19874311101111Yokota, T., T. Hattori, H. Ohishi, K. Hasegawa and K Watanabe,199629751755Hassanein, W.A., E. Kotb, N.M. Awny and Y.A. El-Zawahry,2011Bacillus subtilis K42.]]>36773779Omura, K., M. Hitosugi, X. Zhu, M. Ikeda, H. Maeda and S. Tokududome,2005Bacillus subtilis natto with both antithrombotic and fibrinolytic effects.]]>99247251