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Pakistan Journal of Biological Sciences
  Year: 2013 | Volume: 16 | Issue: 23 | Page No.: 1771-1775
DOI: 10.3923/pjbs.2013.1771.1775
 
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In vivo and in vitro Evaluation of Sterols from Gymnema sylvestrte R. Br

Sharad Vats and Raka Kamal

Abstract:
Gymnema sylvestre R. Br. is an important medicinal plant known for its antidiabetic potential. In the present study, phytosterols from G. sylvestre was identified and quantified in vivo and in vitro. Maximum callus induction was observed in MS medium supplemented with 0.5 mg L-1 of 2, 4-D. The protein content was significantly high both in aerial plant parts and callus tissue. Phytosterols were identified using chromatographic and spectral studies. β-sitosterol, campesterol and stigmasterol were identified both in vivo and in vitro. Lanosterol was identified only in callus culture. Phytosterols have reported for the first time in callus culture of G. sylvestre.
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How to cite this article:

Sharad Vats and Raka Kamal, 2013. In vivo and in vitro Evaluation of Sterols from Gymnema sylvestrte R. Br. Pakistan Journal of Biological Sciences, 16: 1771-1775.

DOI: 10.3923/pjbs.2013.1771.1775

URL: https://scialert.net/abstract/?doi=pjbs.2013.1771.1775

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