Pakistan Journal of Biological Sciences1028-88801812-5735Asian Network for Scientific Information10.3923/pjbs.2013.1771.1775In vivo and in vitro Evaluation of Sterols from Gymnema sylvestrte R. Br]]>VatsSharad KamalRaka 1220131623Gymnema sylvestre R. Br. is an important medicinal plant known for its antidiabetic potential. In the present study, phytosterols from G. sylvestre was identified and quantified in vivo and in vitro. Maximum callus induction was observed in MS medium supplemented with 0.5 mg L-1 of 2, 4-D. The protein content was significantly high both in aerial plant parts and callus tissue. Phytosterols were identified using chromatographic and spectral studies. β-sitosterol, campesterol and stigmasterol were identified both in vivo and in vitro. Lanosterol was identified only in callus culture. Phytosterols have reported for the first time in callus culture of G. sylvestre.]]>Awad, A.B. and C.S. Fink,200013021272130Awad, A.B., K.C. Chan, A.C. Downie and C.S. Fink,200036238241Bone, K.,1997Bradford, P.G. and A.B. Awad,200751161170Calpe-Berdiel, L. and F. Blanco-Vaca,20092031831Dubois, M., K. Gilles, J.K. Hamilton, P.A. Rebers and F. Smith,1951168167167Fazli, F.R.Y. and R. Hardman,1968Trigonella foenum-graecum); its commercial variety of seed as a source of diosgenin.]]>106678Galliard, T.,1968719071914Habib, M.R., F. Nikkon, M. Rahman, M.E. Haque and M.R. Karim, 2007Calotropis gigantean (Linn.). ]]>1041744176Heble, M.R., S. Narayanswami and M.S. Chadha,1968Solanum xanthocarpum tissue cultures.]]>16111451145Kanetkar, P., R. Singhal and M. Kamat,2007Gymnema sylvestre: A memoir.]]>417781Kaufman, B.P., J.L. Cseke, S. Waber, A.J. Duke and L.H. Brielmann,19991st Edn.,Kaul, B. and E.J. Staba,1968Dioscorea tissue cultures. I. Biosynthesis and isolation of diosgenin from Dioscorea deltoidea callus and suspension cultures.]]>31171179Komalavalli, N. and M.V. Rao,2000In vitro micropropagation of Gymnema sylvestre: A multipurpose medicinal plant.]]>6197105Kumar, A.G.J., H.N. Murthy and K.Y. Paek,2002Gymnema sylvestre.]]>718588Law, M.,2000320861864Lowry, O.H., N.J. Rosebrough, A.L. Farr and R.J. Randall,1951193265275Ostlund, Jr. R.E.,200222533549Reddy, P.S., G.R. Gopal and G.L. Sita,1998In vitro multiplication of Gymnema sylvestre R. Br.-An important medicinal plant.]]>75843845Roy, A., S. Ghosh, M. Chaudhuri and P.K. Saha,2008Gymnema sylvestris R. Br. (Asclepiadaceae).]]>722092211Wink, M.,19881988pp: 17-42pp: 17-42Ye, J.C., W.C. Chang, D.J.Y. Hsieh and M.W. Hsiao,20107522527Jayaraman, J.,1981pp: 122-123pp: 122-123